(Circular) (Six-base) MAPSORT of: pFN21AE4143.seq Check: 5426 from: 1 to: 6412 pFN21AE4143 6412 bp With 182 enzymes: SgfI * March 28, 2012 13:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2989 3562 3601 2989 Size: 573 39 5800 Fragments arranged by size: 5800 573 39 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2554 3693 4222 278 Size: 53 83 186 936 1018 1139 529 2468 Fragments arranged by size: 2468 1139 1018 936 529 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 3755 1937 Size: 870 948 4594 Fragments arranged by size: 4594 948 870 Acc65I G'GTAC_C Cuts at: 3733 3733 Size: 6412 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 2399 2522 4475 5859 6127 1632 Size: 704 63 123 1953 1384 268 1917 Fragments arranged by size: 1953 1917 1384 704 268 123 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6215 AflIII A'CryG_T Cuts at: 1203 5311 6288 1203 Size: 4108 977 1327 Fragments arranged by size: 4108 1327 977 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 2080 5141 2080 Size: 3061 3351 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6412 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2855 1184 Size: 1671 4741 AlwNI CAG_nnn'CTG Cuts at: 2389 2666 2770 3037 3262 3265 3607 4155 5727 Size: 277 104 267 225 3 342 548 1572 Cuts at: 5727 2389 Size: 3074 Fragments arranged by size: 3074 1572 548 342 277 267 225 104 3 ApaI G_GGCC'C Cuts at: 3295 3295 Size: 6412 ApaLI G'TGCA_C Cuts at: 4469 5625 4469 Size: 1156 5256 ApoI r'AATT_y Cuts at: 1493 3721 3947 4099 6208 1493 Size: 2228 226 152 2109 1697 Fragments arranged by size: 2228 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4966 160 Size: 872 3934 1606 Fragments arranged by size: 3934 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2394 2604 2825 3737 1870 Size: 73 451 210 221 912 4545 Fragments arranged by size: 4545 912 451 221 210 73 BamHI G'GATC_C Cuts at: 2608 3627 3742 4109 2608 Size: 1019 115 367 4911 Fragments arranged by size: 4911 1019 367 115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2813 3676 3733 5188 Size: 325 217 627 363 663 863 57 1455 Cuts at: 5188 618 Size: 1842 Fragments arranged by size: 1842 1455 863 663 627 363 325 217 57 BanII G_rGCy'C Cuts at: 1391 2124 2777 2952 3165 3203 3295 3731 1391 Size: 733 653 175 213 38 92 436 4072 Fragments arranged by size: 4072 733 653 436 213 175 92 38 BbeI G_GCGC'C Cuts at: 2154 3680 2154 Size: 1526 4886 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5441 BbvCI CC'TCA_GC Cuts at: 2516 2667 3187 3404 2516 Size: 151 520 217 5524 Fragments arranged by size: 5524 520 217 151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4603 4637 1903 Size: 34 2666 34 3678 Fragments arranged by size: 3678 2666 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4304 5514 4304 Size: 1210 5202 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2669 2766 Size: 107 122 71 631 605 337 660 97 Cuts at: 2766 5023 136 Size: 2257 1525 Fragments arranged by size: 2257 1525 660 631 605 337 122 107 97 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 2123 2139 2642 3829 2123 Size: 16 503 1187 4706 Fragments arranged by size: 4706 1187 503 16 Bme1580I G_kGCm'C Cuts at: 1790 2816 3295 4473 5629 1790 Size: 1026 479 1178 1156 2573 Fragments arranged by size: 2573 1178 1156 1026 479 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4143 5101 442 Size: 416 53 83 399 24 2726 958 1753 Fragments arranged by size: 2726 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2272 2812 2272 Size: 540 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2438 2645 2654 2942 3383 3413 3455 Size: 851 348 207 9 288 441 30 42 Cuts at: 3455 3578 3668 5072 1239 Size: 123 90 1404 2579 Fragments arranged by size: 2579 1404 851 441 348 288 207 123 90 42 30 9 Bpu10I CC'TnA_GC Cuts at: 2516 2667 2771 3187 3404 2516 Size: 151 104 416 217 5524 Fragments arranged by size: 5524 416 217 151 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4537 5402 5700 4537 Size: 865 298 5249 Fragments arranged by size: 5249 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3554 3635 5075 882 Size: 617 2055 81 1440 2219 Fragments arranged by size: 2219 2055 1440 617 81 BsaAI yAC'GTr Cuts at: 493 1204 4203 493 Size: 711 2999 2702 Fragments arranged by size: 2999 2702 711 BsaBI GATnn'nnATC Cuts at: 3338 6406 3338 Size: 3068 3344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2151 2551 3677 3690 Size: 53 83 186 936 618 400 1126 13 Cuts at: 3690 4219 4601 5236 275 Size: 529 382 635 1451 Fragments arranged by size: 1451 1126 936 635 618 529 400 382 186 83 53 13 BsaWI w'CCGG_w Cuts at: 4845 5243 5517 5664 6075 4845 Size: 398 274 147 411 5182 Fragments arranged by size: 5182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2684 2714 1178 Size: 30 165 30 1281 30 4876 Fragments arranged by size: 4876 1281 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 2366 2510 2540 2651 2698 2734 3236 Size: 1075 105 144 30 111 47 36 502 Cuts at: 3236 3380 3383 3434 3503 1186 Size: 144 3 51 69 4095 Fragments arranged by size: 4095 1075 502 144 144 111 105 69 51 47 36 30 3 BseYI C'CCAG_C Cuts at: 2368 3236 3498 5615 6111 2368 Size: 868 262 2117 496 2669 Fragments arranged by size: 2669 2117 868 496 262 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2049 2249 2558 3101 3107 3256 3431 Size: 573 313 200 309 543 6 149 175 Cuts at: 3431 3469 3497 3596 1163 Size: 38 28 99 3979 Fragments arranged by size: 3979 573 543 313 309 200 175 149 99 38 28 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2057 3862 4623 5651 1540 Size: 275 186 56 1805 761 1028 2301 Fragments arranged by size: 2301 1805 1028 761 275 186 56 BsiHKAI G_wGCw'C Cuts at: 2124 2204 2952 3006 3315 4473 4558 5629 2124 Size: 80 748 54 309 1158 85 1071 2907 Fragments arranged by size: 2907 1158 1071 748 309 85 80 54 BsmI GAATG_Cn' Cuts at: 3923 4016 3923 Size: 93 6319 BsmBI CGTCTCn'nnnn_ Cuts at: 2542 2783 2959 2542 Size: 241 176 5995 Fragments arranged by size: 5995 241 176 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2204 2777 2816 2952 3006 3165 Size: 399 334 80 573 39 136 54 159 Cuts at: 3165 3203 3295 3315 3731 4473 4558 5629 1391 Size: 38 92 20 416 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 416 399 334 159 136 92 85 80 54 39 38 20 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 4301 6031 4301 Size: 1730 4682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2847 2989 3562 3601 3767 844 Size: 2003 142 573 39 166 3489 Fragments arranged by size: 3489 2003 573 166 142 39 BsrBI CCG'CTC Cuts at: 1394 1823 2044 2362 2646 2941 3076 3472 3625 Size: 429 221 318 284 295 135 396 153 Cuts at: 3625 4299 1394 Size: 674 3507 Fragments arranged by size: 3507 674 429 396 318 295 284 221 153 135 BsrDI GCAATG_nn' Cuts at: 65 1248 2980 4907 5081 65 Size: 1183 1732 1927 174 1396 Fragments arranged by size: 1927 1732 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2127 5056 5243 1700 Size: 110 210 107 2929 187 2869 Fragments arranged by size: 2929 2869 210 187 110 107 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6412 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 2441 4472 5484 2441 Size: 2031 1012 3369 Fragments arranged by size: 3369 2031 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3037 3262 794 Size: 2243 225 3944 Fragments arranged by size: 3944 2243 225 BstBI TT'CG_AA Cuts at: 5216 5216 Size: 6412 BstEII G'GTnAC_C Cuts at: 1184 3103 1184 Size: 1919 4493 BstYI r'GATC_y Cuts at: 1972 2541 2608 3627 3742 4109 4494 4511 5963 Size: 569 67 1019 115 367 385 17 1452 Cuts at: 5963 6049 6061 6407 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1019 569 385 367 346 115 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 3455 3455 Size: 6412 BtgI C'CryG_G Cuts at: 513 1065 2004 2028 2478 2583 513 Size: 552 939 24 450 105 4342 Fragments arranged by size: 4342 939 552 450 105 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2598 2703 487 Size: 683 56 1372 105 4196 Fragments arranged by size: 4196 1372 683 105 56 BtsI GCAGTG_nn' Cuts at: 2243 3230 3928 4722 4742 2243 Size: 987 698 794 20 3913 Fragments arranged by size: 3913 987 794 698 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6377 DraI TTT'AAA Cuts at: 3716 3716 Size: 6412 DraIII CAC_nnn'GTG Cuts at: 2877 2877 Size: 6412 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2054 3378 Size: 54 1425 50 275 18 195 29 1324 Cuts at: 3378 3859 4748 6377 8 Size: 481 889 1629 43 Fragments arranged by size: 1629 1425 1324 889 481 275 195 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2054 3859 1537 Size: 275 242 1805 4090 Fragments arranged by size: 4090 1805 275 242 EarI CTCTTCn'nnn_ Cuts at: 1353 2781 4342 1353 Size: 1428 1561 3423 Fragments arranged by size: 3423 1561 1428 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3110 3620 4999 5373 5519 387 Size: 1064 138 1521 510 1379 374 146 1280 Fragments arranged by size: 1521 1379 1280 1064 510 374 146 138 EcoICRI GAG'CTC Cuts at: 2122 2950 2122 Size: 828 5584 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2336 2399 2438 2522 2645 2654 Size: 393 458 246 63 39 84 123 9 Cuts at: 2654 2942 3383 3413 3455 3578 3668 4475 5072 Size: 288 441 30 42 123 90 807 597 Cuts at: 5072 5859 6127 1239 Size: 787 268 1524 Fragments arranged by size: 1524 807 787 597 458 441 393 288 268 246 123 123 90 84 63 42 39 30 9 EcoNI CCTnn'n_nnAGG Cuts at: 2318 3011 2318 Size: 693 5719 EcoO109I rG'GnC_Cy Cuts at: 2767 2917 2959 3291 3604 2767 Size: 150 42 332 313 5575 Fragments arranged by size: 5575 332 313 150 42 EcoRI G'AATT_C Cuts at: 3721 4099 3721 Size: 378 6034 FspI TGC'GCA Cuts at: 3659 4918 3659 Size: 1259 5153 HaeII r_GCGC'y Cuts at: 1678 2154 2700 3328 3680 5559 1678 Size: 476 546 628 352 1879 2531 Fragments arranged by size: 2531 1879 628 546 476 352 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2096 2098 2128 2684 Size: 32 135 32 494 30 2 30 556 Cuts at: 2684 2716 2917 2949 3212 3244 3500 3532 5053 Size: 32 201 32 263 32 256 32 1521 Cuts at: 5053 5085 5127 5159 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1521 556 494 263 256 201 135 42 32 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3756 4002 669 Size: 988 281 1818 246 3079 Fragments arranged by size: 3079 1818 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KasI G'GCGC_C Cuts at: 2150 3676 2150 Size: 1526 4886 KpnI G_GTAC'C Cuts at: 3737 3737 Size: 6412 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5526 5710 1305 Size: 4221 184 2007 Fragments arranged by size: 4221 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3380 10 Size: 54 1425 343 1548 3042 Fragments arranged by size: 3042 1548 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4370 4729 4888 518 Size: 648 3204 359 159 2042 Fragments arranged by size: 3204 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2030 2233 2386 2623 2666 2887 2908 Size: 409 15 203 153 237 43 221 21 Cuts at: 2908 3034 3085 3178 3262 3403 3415 3828 4505 Size: 126 51 93 84 141 12 413 677 Cuts at: 4505 5653 5898 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 413 409 245 237 221 203 153 141 126 93 84 51 43 21 15 12 NaeI GCC'GGC Cuts at: 2129 2129 Size: 6412 NarI GG'CG_CC Cuts at: 2151 3677 2151 Size: 1526 4886 NcoI C'CATG_G Cuts at: 513 1065 2004 2478 513 Size: 552 939 474 4447 Fragments arranged by size: 4447 939 552 474 NdeI CA'TA_TG Cuts at: 387 387 Size: 6412 NgoMIV G'CCGG_C Cuts at: 2127 2127 Size: 6412 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NruI TCG'CGA Cuts at: 2937 2937 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2564 3770 5315 1132 Size: 97 1335 1206 1545 2229 Fragments arranged by size: 2229 1545 1335 1206 97 PasI CC'CwG_GG Cuts at: 1348 3196 1348 Size: 1848 4564 PciI A'CATG_T Cuts at: 5311 5311 Size: 6412 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2917 2917 Size: 6412 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 3982 3982 Size: 6412 PspOMI G'GGCC_C Cuts at: 3291 3291 Size: 6412 PstI C_TGCA'G Cuts at: 830 2265 2892 2913 3090 3258 3303 3549 3612 Size: 1435 627 21 177 168 45 246 63 Cuts at: 3612 3764 830 Size: 152 3478 Fragments arranged by size: 3478 1435 627 246 177 168 152 63 45 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 PvuII CAG'CTG Cuts at: 2015 2386 2887 2908 3034 3085 3178 3262 3403 Size: 371 501 21 126 51 93 84 141 Cuts at: 3403 2015 Size: 5024 Fragments arranged by size: 5024 501 371 141 126 93 84 51 21 SacI G_AGCT'C Cuts at: 2124 2952 2124 Size: 828 5584 SacII CC_GC'GG Cuts at: 2031 2031 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SbfI CC_TGCA'GG Cuts at: 3612 3764 3612 Size: 152 6260 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 2261 2888 2909 3086 3254 3299 3545 Size: 219 1216 627 21 177 168 45 246 Cuts at: 3545 3608 3760 4895 5576 5767 826 Size: 63 152 1135 681 191 1471 Fragments arranged by size: 1471 1216 1135 681 627 246 219 191 177 168 152 63 45 21 SfoI GGC'GCC Cuts at: 2152 3678 2152 Size: 1526 4886 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 2020 1700 Size: 320 6092 SmaI CCC'GGG Cuts at: 1872 2396 2606 3739 1872 Size: 524 210 1133 4545 Fragments arranged by size: 4545 1133 524 210 SmlI C'TyrA_G Cuts at: 820 1017 1943 4516 5417 5679 820 Size: 197 926 2573 901 262 1553 Fragments arranged by size: 2573 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 2564 3770 1132 Size: 1432 1206 3774 Fragments arranged by size: 3774 1432 1206 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 2672 3440 1517 Size: 1155 768 4489 Fragments arranged by size: 4489 1155 768 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 2719 3157 3531 3851 513 Size: 552 939 474 241 438 374 320 3074 Fragments arranged by size: 3074 939 552 474 438 374 320 241 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3125 4439 4456 4609 4794 5239 325 Size: 1255 1545 1314 17 153 185 445 1498 Fragments arranged by size: 1545 1498 1314 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4658 6370 96 Size: 274 80 33 51 1443 2681 1712 138 Fragments arranged by size: 2681 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2806 2838 6372 6404 2806 Size: 32 3534 32 2814 Fragments arranged by size: 3534 2814 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 3748 3748 Size: 6412 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2874 3388 2179 Size: 695 514 5203 Fragments arranged by size: 5203 695 514 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6412 XmaI C'CCGG_G Cuts at: 1870 2394 2604 3737 1870 Size: 524 210 1133 4545 Fragments arranged by size: 4545 1133 524 210 XmnI GAAnn'nnTTC Cuts at: 4541 4541 Size: 6412 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2552 3691 4220 276 Size: 53 83 186 936 1018 1139 529 2468 Fragments arranged by size: 2468 1139 1018 936 529 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I EcoRV FalI FseI FspAI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI