Restriction map for
pFN21AE4143
(Linear) (Six-base) MAPSORT of: KIEE4143.seq Check: 9783 from: 1 to: 1707
KIEE4143.orf
With 182 enzymes: SgfI *
March 22, 2012 17:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 984 1557 1596 1707
Size: 984 573 39 111
Fragments arranged by size:
984 573 111 39
AatII G_ACGT'C
Cuts at: 0 549 1688 1707
Size: 549 1139 19
Fragments arranged by size:
1139 549 19
AccI GT'mk_AC
Cuts at: 0 802 1707
Size: 802 905
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 394 517 1707
Size: 331 63 123 1190
Fragments arranged by size:
1190 331 123 63
AhdI GACnn_n'nnGTC
Cuts at: 0 75 1707
Size: 75 1632
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 1707
Size: 850 857
AlwNI CAG_nnn'CTG
Cuts at: 0 384 661 765 1032 1257 1260 1602 1707
Size: 384 277 104 267 225 3 342 105
Fragments arranged by size:
384 342 277 267 225 105 104 3
ApaI G_GGCC'C
Cuts at: 0 1290 1707
Size: 1290 417
AvaI C'yCGr_G
Cuts at: 0 389 599 820 1707
Size: 389 210 221 887
Fragments arranged by size:
887 389 221 210
BamHI G'GATC_C
Cuts at: 0 603 1622 1707
Size: 603 1019 85
Fragments arranged by size:
1019 603 85
BanI G'GyrC_C
Cuts at: 0 145 808 1671 1707
Size: 145 663 863 36
Fragments arranged by size:
863 663 145 36
BanII G_rGCy'C
Cuts at: 0 119 772 947 1160 1198 1290 1707
Size: 119 653 175 213 38 92 417
Fragments arranged by size:
653 417 213 175 119 92 38
BbeI G_GCGC'C
Cuts at: 0 149 1675 1707
Size: 149 1526 32
Fragments arranged by size:
1526 149 32
BbvCI CC'TCA_GC
Cuts at: 0 511 662 1182 1399 1707
Size: 511 151 520 217 308
Fragments arranged by size:
520 511 308 217 151
BglI GCCn_nnn'nGGC
Cuts at: 0 664 761 1707
Size: 664 97 946
Fragments arranged by size:
946 664 97
BlpI GC'TnA_GC
Cuts at: 0 118 134 637 1707
Size: 118 16 503 1070
Fragments arranged by size:
1070 503 118 16
Bme1580I G_kGCm'C
Cuts at: 0 811 1290 1707
Size: 811 479 417
Fragments arranged by size:
811 479 417
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 267 807 1707
Size: 267 540 900
Fragments arranged by size:
900 540 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 433 640 649 937 1378 1408 1450
Size: 85 348 207 9 288 441 30 42
Cuts at: 1450 1573 1663 1707
Size: 123 90 44
Fragments arranged by size:
441 348 288 207 123 90 85 44
42 30 9
Bpu10I CC'TnA_GC
Cuts at: 0 511 662 766 1182 1399 1707
Size: 511 151 104 416 217 308
Fragments arranged by size:
511 416 308 217 151 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 1549 1630 1707
Size: 1549 81 77
Fragments arranged by size:
1549 81 77
BsaBI GATnn'nnATC
Cuts at: 0 1333 1707
Size: 1333 374
BsaHI Gr'CG_yC
Cuts at: 0 146 546 1672 1685 1707
Size: 146 400 1126 13 22
Fragments arranged by size:
1126 400 146 22 13
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 679 709 1707
Size: 679 30 998
Fragments arranged by size:
998 679 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 361 505 535 646 693 729 1231
Size: 256 105 144 30 111 47 36 502
Cuts at: 1231 1375 1378 1429 1498 1707
Size: 144 3 51 69 209
Fragments arranged by size:
502 256 209 144 144 111 105 69
51 47 36 30 3
BseYI C'CCAG_C
Cuts at: 0 363 1231 1493 1707
Size: 363 868 262 214
Fragments arranged by size:
868 363 262 214
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 244 553 1096 1102 1251 1426 1464
Size: 44 200 309 543 6 149 175 38
Cuts at: 1464 1492 1591 1707
Size: 28 99 116
Fragments arranged by size:
543 309 200 175 149 116 99 44
38 28 6
BsiEI CG_ry'CG
Cuts at: 0 52 1707
Size: 52 1655
BsiHKAI G_wGCw'C
Cuts at: 0 119 199 947 1001 1310 1707
Size: 119 80 748 54 309 397
Fragments arranged by size:
748 397 309 119 80 54
BsmBI CGTCTCn'nnnn_
Cuts at: 0 537 778 954 1707
Size: 537 241 176 753
Fragments arranged by size:
753 537 241 176
Bsp1286I G_dGCh'C
Cuts at: 0 119 199 772 811 947 1001 1160 1198
Size: 119 80 573 39 136 54 159 38
Cuts at: 1198 1290 1310 1707
Size: 92 20 397
Fragments arranged by size:
573 397 159 136 119 92 80 54
39 38 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 842 984 1557 1596 1707
Size: 842 142 573 39 111
Fragments arranged by size:
842 573 142 111 39
BsrBI CCG'CTC
Cuts at: 0 39 357 641 936 1071 1467 1620 1707
Size: 39 318 284 295 135 396 153 87
Fragments arranged by size:
396 318 295 284 153 135 87 39
BsrDI GCAATG_nn'
Cuts at: 0 975 1707
Size: 975 732
BsrFI r'CCGG_y
Cuts at: 0 15 122 1707
Size: 15 107 1585
Fragments arranged by size:
1585 107 15
BssSI C'ACGA_G
Cuts at: 0 436 1707
Size: 436 1271
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1032 1257 1707
Size: 1032 225 450
Fragments arranged by size:
1032 450 225
BstEII G'GTnAC_C
Cuts at: 0 1098 1707
Size: 1098 609
BstYI r'GATC_y
Cuts at: 0 536 603 1622 1707
Size: 536 67 1019 85
Fragments arranged by size:
1019 536 85 67
Bsu36I CC'TnA_GG
Cuts at: 0 1450 1707
Size: 1450 257
BtgI C'CryG_G
Cuts at: 0 23 473 578 1707
Size: 23 450 105 1129
Fragments arranged by size:
1129 450 105 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 593 698 1707
Size: 593 105 1009
Fragments arranged by size:
1009 593 105
BtsI GCAGTG_nn'
Cuts at: 0 238 1225 1707
Size: 238 987 482
Fragments arranged by size:
987 482 238
DraIII CAC_nnn'GTG
Cuts at: 0 872 1707
Size: 872 835
EaeI y'GGCC_r
Cuts at: 0 20 49 1373 1707
Size: 20 29 1324 334
Fragments arranged by size:
1324 334 29 20
EagI C'GGCC_G
Cuts at: 0 49 1707
Size: 49 1658
EarI CTCTTCn'nnn_
Cuts at: 0 776 1707
Size: 776 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1105 1615 1707
Size: 1105 510 92
Fragments arranged by size:
1105 510 92
EcoICRI GAG'CTC
Cuts at: 0 117 945 1707
Size: 117 828 762
Fragments arranged by size:
828 762 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 331 394 433 517 640 649 937
Size: 85 246 63 39 84 123 9 288
Cuts at: 937 1378 1408 1450 1573 1663 1707
Size: 441 30 42 123 90 44
Fragments arranged by size:
441 288 246 123 123 90 85 84
63 44 42 39 30 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1006 1707
Size: 313 693 701
Fragments arranged by size:
701 693 313
EcoO109I rG'GnC_Cy
Cuts at: 0 762 912 954 1286 1599 1707
Size: 762 150 42 332 313 108
Fragments arranged by size:
762 332 313 150 108 42
FspI TGC'GCA
Cuts at: 0 1654 1707
Size: 1654 53
HaeII r_GCGC'y
Cuts at: 0 149 695 1323 1675 1707
Size: 149 546 628 352 32
Fragments arranged by size:
628 546 352 149 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 91 93 123 679 711 912 944
Size: 61 30 2 30 556 32 201 32
Cuts at: 944 1207 1239 1495 1527 1707
Size: 263 32 256 32 180
Fragments arranged by size:
556 263 256 201 180 61 32 32
32 32 30 30 2
KasI G'GCGC_C
Cuts at: 0 145 1671 1707
Size: 145 1526 36
Fragments arranged by size:
1526 145 36
MscI TGG'CCA
Cuts at: 0 1375 1707
Size: 1375 332
MspA1I CmG'CkG
Cuts at: 0 10 25 228 381 618 661 882 903
Size: 10 15 203 153 237 43 221 21
Cuts at: 903 1029 1080 1173 1257 1398 1410 1707
Size: 126 51 93 84 141 12 297
Fragments arranged by size:
297 237 221 203 153 141 126 93
84 51 43 21 15 12 10
NaeI GCC'GGC
Cuts at: 0 124 1707
Size: 124 1583
NarI GG'CG_CC
Cuts at: 0 146 1672 1707
Size: 146 1526 35
Fragments arranged by size:
1526 146 35
NcoI C'CATG_G
Cuts at: 0 473 1707
Size: 473 1234
NgoMIV G'CCGG_C
Cuts at: 0 122 1707
Size: 122 1585
NruI TCG'CGA
Cuts at: 0 932 1707
Size: 932 775
NspI r_CATG'y
Cuts at: 0 559 1707
Size: 559 1148
PasI CC'CwG_GG
Cuts at: 0 1191 1707
Size: 1191 516
PpuMI rG'GwC_Cy
Cuts at: 0 912 1707
Size: 912 795
PspOMI G'GGCC_C
Cuts at: 0 1286 1707
Size: 1286 421
PstI C_TGCA'G
Cuts at: 0 260 887 908 1085 1253 1298 1544 1607
Size: 260 627 21 177 168 45 246 63
Cuts at: 1607 1707
Size: 100
Fragments arranged by size:
627 260 246 177 168 100 63 45
21
PvuII CAG'CTG
Cuts at: 0 10 381 882 903 1029 1080 1173 1257
Size: 10 371 501 21 126 51 93 84
Cuts at: 1257 1398 1707
Size: 141 309
Fragments arranged by size:
501 371 309 141 126 93 84 51
21 10
SacI G_AGCT'C
Cuts at: 0 119 947 1707
Size: 119 828 760
Fragments arranged by size:
828 760 119
SacII CC_GC'GG
Cuts at: 0 26 1707
Size: 26 1681
SbfI CC_TGCA'GG
Cuts at: 0 1607 1707
Size: 1607 100
SfcI C'TryA_G
Cuts at: 0 256 883 904 1081 1249 1294 1540 1603
Size: 256 627 21 177 168 45 246 63
Cuts at: 1603 1707
Size: 104
Fragments arranged by size:
627 256 246 177 168 104 63 45
21
SfoI GGC'GCC
Cuts at: 0 147 1673 1707
Size: 147 1526 34
Fragments arranged by size:
1526 147 34
SgrAI Cr'CCGG_yG
Cuts at: 0 15 1707
Size: 15 1692
SmaI CCC'GGG
Cuts at: 0 391 601 1707
Size: 391 210 1106
Fragments arranged by size:
1106 391 210
SphI G_CATG'C
Cuts at: 0 559 1707
Size: 559 1148
StuI AGG'CCT
Cuts at: 0 667 1435 1707
Size: 667 768 272
Fragments arranged by size:
768 667 272
StyI C'CwwG_G
Cuts at: 0 473 714 1152 1526 1707
Size: 473 241 438 374 181
Fragments arranged by size:
473 438 374 241 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1120 1707
Size: 1120 587
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 801 833 1707
Size: 801 32 874
Fragments arranged by size:
874 801 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 869 1383 1707
Size: 174 695 514 324
Fragments arranged by size:
695 514 324 174
XmaI C'CCGG_G
Cuts at: 0 389 599 1707
Size: 389 210 1108
Fragments arranged by size:
1108 389 210
ZraI GAC'GTC
Cuts at: 0 547 1686 1707
Size: 547 1139 21
Fragments arranged by size:
1139 547 21
Enzymes that do cut:
AarI AatII AccI AcuI AhdI AlfI AlwNI ApaI
AvaI BamHI BanI BanII BbeI BbvCI BglI BlpI
Bme1580I BplI BpmI Bpu10I BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI
MscI MspA1I NaeI NarI NcoI NgoMIV NruI NspI
PasI PpuMI PspOMI PstI PvuII SacI SacII SbfI
SfcI SfoI SgrAI SmaI SphI StuI StyI TaqII
TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI
BpuEI BsaAI BsaWI BsiWI BsmI BspEI BspHI BsrGI
BssHII BstBI BstXI BstZ17I ClaI CspCI DraI DrdI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI MslI NdeI NheI NotI
NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SapI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI