Restriction map for
pFN21AE5685
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI BanI BbeI BcgI BfrI
BglII BsaBI BsaHI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI HgaI Hin1I
HindIII KasI KpnI Ksp632I KspI
MamI MfeI MluI MslI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 1241 TCCGGA T^CCGGA
AcsI 170 GAATTC [GA]^AATT[CT]
AflIII 76 ACGTGT A^C[GA][CT]GT
AlwNI 968 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1450 AGCGCT AGC^GCT
ApoI 170 GAATTC [GA]^AATT[CT]
AviII 243 TGCGCA TGC^GCA
AvrII 414 CCTAGG C^CTAGG
BamHI 447 GGATCC G^GATCC
BbrPI 75 CACGTG CAC^GTG
BbsI 1078 GTCTTC GAAGAC(2/6)
BciVI 1389 GTATCC GTATCC(6/5)
BclI 177 TGATCA T^GATCA
BglI 1139 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 414 CCTAGG C^CTAGG
BlpI 1134 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1134 GCTGAGC GC^T[ACGT]AGC
BpuAI 1078 GTCTTC GAAGAC(2/6)
BsaAI 75 CACGTG [CT]AC^GT[GA]
BsaWI 1241 TCCGGA [AT]^CCGG[AT]
BseAI 1241 TCCGGA T^CCGGA
BseRI 793 CTCCTC GAGGAG(10/8)
BsmI 1301 GAATGC GAATGC(1/-1)
BspEI 1241 TCCGGA T^CCGGA
BstAPI 1296 GCATGGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 447 GGATCC [GA]^GATC[CT]
CelII 1134 GCTGAGC GC^T[ACGT]AGC
DraI 672 TTTAAA TTT^AAA
DsaI 258 CCGTGG C^C[GA][CT]GG
Eco47III 1450 AGCGCT AGC^GCT
EcoNI 1054 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 170 GAATTC G^AATTC
FbaI 177 TGATCA T^GATCA
FspI 243 TGCGCA TGC^GCA
HaeII 1450 AGCGCT [GA]GCGC^[CT]
HincII 982 GTTAAC GT[CT]^[GA]AC
HindII 982 GTTAAC GT[CT]^[GA]AC
HpaI 982 GTTAAC GTT^AAC
HphI 1206 GGTGA GGTGA(8/7)
MaeII 76 ACGT A^CGT
MflI 447 GGATCC [GA]^GATC[CT]
MroI 1241 TCCGGA T^CCGGA
NspI 386 GCATGC [GA]CATG^[CT]
PmaCI 75 CACGTG CAC^GTG
PmlI 75 CACGTG CAC^GTG
PpuMI 788 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 857 CAGCTG CAG^CTG
SmlI 429 CTTGAG C^T[CT][GA]AG
SphI 386 GCATGC GCATG^C
SspI 1157 AATATT AAT^ATT
XhoII 447 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 379 GTAC GT^AC
878 GTAC
AspHI 333 GAGCAC G[AT]GC[AT]^C
1289 GTGCTC
BanII 1137 GAGCCC G[GA]GC[CT]^C
1245 GAGCCC
BbvCI 353 CCTCAGC CCTCAGC(-5/-2)
1312 GCTGAGG
BpmI 330 CTGGAG CTGGAG(16/14)
882 CTGGAG
BsaI 665 GAGACC GGTCTC(1/5)
689 GGTCTC
BsiHKAI 333 GAGCAC G[AT]GC[AT]^C
1289 GTGCTC
BspHI 174 TCATGA T^CATGA
1125 TCATGA
BspMI 63 ACCTGC ACCTGC(4/8)
1203 GCAGGT
BstBI 98 TTCGAA TT^CGAA
1081 TTCGAA
FauI 9 CCCGC CCCGC(4/6)
1131 CCCGC
MseI 673 TTAA T^TAA
983 TTAA
NspV 98 TTCGAA TT^CGAA
1081 TTCGAA
RcaI 174 TCATGA T^CATGA
1125 TCATGA
RsaI 379 GTAC GT^AC
878 GTAC
SfuI 98 TTCGAA TT^CGAA
1081 TTCGAA
Tru9I 673 TTAA T^TAA
983 TTAA
Tsp45I 727 GTCAC ^GT[GC]AC
1411 GTGAC
Tsp509I 124 AATT ^AATT
171 AATT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AluI AlwI AvaII BalI
BbvI BcnI BfaI BmyI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
TspRI TthHB8I