(Circular) (Six-base) MAPSORT of: pFN21AE5685.seq Check: 441 from: 1 to: 6388 pFN21AE5685 6388 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2077 3199 2077 Size: 1122 5266 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3731 1937 Size: 1794 4594 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2229 2560 2806 3101 4451 5835 6103 1632 Size: 597 331 246 295 1350 1384 268 1917 Fragments arranged by size: 1917 1384 1350 597 331 295 268 246 AfeI AGC'GCT Cuts at: 3457 3457 Size: 6388 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 2081 5287 6264 1203 Size: 878 3206 977 1327 Fragments arranged by size: 3206 1327 977 878 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3221 1184 Size: 2037 4351 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3665 3697 3665 Size: 32 6356 AlwNI CAG_nnn'CTG Cuts at: 2978 4131 5703 2978 Size: 1153 1572 3663 Fragments arranged by size: 3663 1572 1153 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 2175 3697 3923 4075 6184 1493 Size: 682 1522 226 152 2109 1697 Fragments arranged by size: 2109 1697 1522 682 226 152 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 3713 1870 Size: 73 1770 4545 Fragments arranged by size: 4545 1770 73 AvrII C'CTAG_G Cuts at: 2419 2419 Size: 6388 BamHI G'GATC_C Cuts at: 2452 3718 4085 2452 Size: 1266 367 4755 Fragments arranged by size: 4755 1266 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3709 5164 618 Size: 325 217 627 1922 1455 1842 Fragments arranged by size: 1922 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3146 3254 3707 1391 Size: 1755 108 453 4072 Fragments arranged by size: 4072 1755 453 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3076 928 Size: 971 1177 4240 Fragments arranged by size: 4240 1177 971 BbvCI CC'TCA_GC Cuts at: 2359 3318 2359 Size: 959 5429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3405 4280 5490 3405 Size: 875 1210 4303 Fragments arranged by size: 4303 1210 875 BclI T'GATC_A Cuts at: 1548 2182 1548 Size: 634 5754 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3150 4999 136 Size: 107 122 71 631 605 1478 1849 1525 Fragments arranged by size: 1849 1525 1478 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 3140 3805 3140 Size: 665 5723 Bme1580I G_kGCm'C Cuts at: 1790 4449 5605 1790 Size: 2659 1156 2573 Fragments arranged by size: 2659 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2417 4119 5077 Size: 416 53 83 399 24 1000 1702 958 Cuts at: 5077 442 Size: 1753 Fragments arranged by size: 1753 1702 1000 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3273 3273 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2356 2908 5048 1239 Size: 1117 552 2140 2579 Fragments arranged by size: 2579 2140 1117 552 Bpu10I CC'TnA_GC Cuts at: 2359 3318 2359 Size: 959 5429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2455 4513 5378 5676 2455 Size: 2058 865 298 3167 Fragments arranged by size: 3167 2058 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2664 2700 5051 882 Size: 617 1165 36 2351 2219 Fragments arranged by size: 2351 2219 1165 617 36 BsaAI yAC'GTr Cuts at: 493 1204 2082 4179 493 Size: 711 878 2097 2702 Fragments arranged by size: 2702 2097 878 711 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 4577 5212 275 Size: 53 83 186 936 2662 382 635 1451 Fragments arranged by size: 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3246 4821 5219 5493 5640 6051 3246 Size: 1575 398 274 147 411 3583 Fragments arranged by size: 3583 1575 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2763 2793 1178 Size: 30 165 30 1360 30 4773 Fragments arranged by size: 4773 1360 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 1186 Size: 1603 4785 BseYI C'CCAG_C Cuts at: 2049 2322 3272 5591 6087 2049 Size: 273 950 2319 496 2350 Fragments arranged by size: 2350 2319 950 496 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2137 2831 3227 1163 Size: 573 401 694 396 4324 Fragments arranged by size: 4324 694 573 401 396 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2342 3298 4449 4534 5605 2342 Size: 956 1151 85 1071 3125 Fragments arranged by size: 3125 1151 1071 956 85 BsmI GAATG_Cn' Cuts at: 3312 3899 3992 3312 Size: 587 93 5708 Fragments arranged by size: 5708 587 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2342 3146 3254 3298 3707 4449 4534 Size: 399 552 804 108 44 409 742 85 Cuts at: 4534 5605 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 804 742 552 409 399 108 85 44 BspEI T'CCGG_A Cuts at: 3246 6051 3246 Size: 2805 3583 BspHI T'CATG_A Cuts at: 2179 3130 4277 6007 2179 Size: 951 1147 1730 2560 Fragments arranged by size: 2560 1730 1147 951 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2077 3199 3743 844 Size: 1233 1122 544 3489 Fragments arranged by size: 3489 1233 1122 544 BsrBI CCG'CTC Cuts at: 1394 1823 4275 1394 Size: 429 2452 3507 Fragments arranged by size: 3507 2452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5032 5219 1700 Size: 110 3222 187 2869 Fragments arranged by size: 3222 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 3307 794 Size: 2513 3875 BstBI TT'CG_AA Cuts at: 2104 3087 5192 2104 Size: 983 2105 3300 Fragments arranged by size: 3300 2105 983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstYI r'GATC_y Cuts at: 1972 2452 3718 4085 4470 4487 5939 6025 6037 Size: 480 1266 367 385 17 1452 86 12 Cuts at: 6037 6383 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1266 480 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2263 513 Size: 552 939 259 4638 Fragments arranged by size: 4638 939 552 259 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 3904 4698 4718 3904 Size: 794 20 5574 Fragments arranged by size: 5574 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 2679 3692 2679 Size: 1013 5375 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2765 2783 2893 Size: 54 1425 50 275 18 935 18 110 Cuts at: 2893 3835 4724 6353 8 Size: 942 889 1629 43 Fragments arranged by size: 1629 1425 942 935 889 275 110 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4318 1353 Size: 2965 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4975 5349 5495 387 Size: 1064 138 3386 374 146 1280 Fragments arranged by size: 3386 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2356 2560 2806 2908 3101 4451 Size: 393 597 127 204 246 102 193 1350 Cuts at: 4451 5048 5835 6103 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1350 787 597 597 393 268 246 204 193 127 102 EcoNI CCTnn'n_nnAGG Cuts at: 3063 3063 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 2794 3322 3634 2794 Size: 528 312 5548 Fragments arranged by size: 5548 528 312 EcoRI G'AATT_C Cuts at: 2175 3697 4075 2175 Size: 1522 378 4488 Fragments arranged by size: 4488 1522 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2738 2738 Size: 6388 FspI TGC'GCA Cuts at: 2250 4894 2250 Size: 2644 3744 HaeII r_GCGC'y Cuts at: 1678 3459 5535 1678 Size: 1781 2076 2531 Fragments arranged by size: 2531 2076 1781 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2525 2557 2582 2614 3564 Size: 32 135 32 953 32 25 32 950 Cuts at: 3564 3596 5029 5061 5103 5135 1373 Size: 32 1433 32 42 32 2626 Fragments arranged by size: 2626 1433 953 950 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 2989 3732 3978 669 Size: 988 281 1051 743 246 3079 Fragments arranged by size: 3079 1051 988 743 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 2989 3978 2989 Size: 989 5399 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5502 5686 1305 Size: 4197 184 2007 Fragments arranged by size: 4197 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2767 2785 2895 10 Size: 54 1425 343 935 18 110 3503 Fragments arranged by size: 3503 1425 935 343 110 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 4346 4705 4864 518 Size: 648 3180 359 159 2042 Fragments arranged by size: 3180 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2017 2864 3139 3804 4481 5629 5874 1606 Size: 411 847 275 665 677 1148 245 2120 Fragments arranged by size: 2120 1148 847 677 665 411 275 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4897 Fragments arranged by size: 4897 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 2395 3746 5291 1132 Size: 97 1166 1351 1545 2229 Fragments arranged by size: 2229 1545 1351 1166 97 PasI CC'CwG_GG Cuts at: 1348 3219 1348 Size: 1871 4517 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6388 PfoI T'CCnGG_A Cuts at: 2199 2529 2199 Size: 330 6058 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 2082 2082 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 2794 Size: 6388 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2912 2944 2912 Size: 32 6356 PstI C_TGCA'G Cuts at: 830 2304 2731 2863 3545 3740 830 Size: 1474 427 132 682 195 3478 Fragments arranged by size: 3478 1474 682 427 195 132 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2864 2864 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2300 2727 2859 3541 3736 4871 5552 Size: 219 1255 427 132 682 195 1135 681 Cuts at: 5552 5743 826 Size: 191 1471 Fragments arranged by size: 1471 1255 1135 682 681 427 219 195 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2434 4492 5393 5655 820 Size: 197 926 491 2058 901 262 1553 Fragments arranged by size: 2058 1553 926 901 491 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 2395 3746 1132 Size: 1263 1351 3774 Fragments arranged by size: 3774 1351 1263 SspI AAT'ATT Cuts at: 5 52 3164 4312 5 Size: 47 3112 1148 2081 Fragments arranged by size: 3112 2081 1148 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 2004 2293 2419 2458 2960 3827 513 Size: 552 939 289 126 39 502 867 3074 Fragments arranged by size: 3074 939 867 552 502 289 126 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2343 2357 4415 4432 4585 4770 5215 Size: 1255 763 14 2058 17 153 185 445 Cuts at: 5215 325 Size: 1498 Fragments arranged by size: 2058 1498 1255 763 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4634 6346 96 Size: 274 80 33 51 1443 2657 1712 138 Fragments arranged by size: 2657 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2763 2795 6348 6380 2763 Size: 32 3553 32 2771 Fragments arranged by size: 3553 2771 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6388 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI