################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BglI BglII BlnI BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FokI FseI FspI HaeII HgaI Hin1I HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccI 54 GTCTAC GT^[AC][GT]AC AfaI 41 GTAC GT^AC AflII 919 CTTAAG C^TTAAG AlwI 679 GATCC GGATC(4/5) AlwNI 143 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 520 GAGCTC G[AT]GC[AT]^C AvaI 314 CTCGGG C^[CT]CG[GA]G BclI 729 TGATCA T^GATCA BfrI 919 CTTAAG C^TTAAG BlpI 282 GCTGAGC GC^T[ACGT]AGC Bpu1102I 282 GCTGAGC GC^T[ACGT]AGC BsaI 697 GAGACC GGTCTC(1/5) BsaWI 92 ACCGGA [AT]^CCGG[AT] BsiHKAI 520 GAGCTC G[AT]GC[AT]^C BsmBI 423 GAGACG CGTCTC(1/5) BsmFI 641 GGGAC GGGAC(10/14) BsoBI 314 CTCGGG C^[CT]CG[GA]G BsrBI 285 GAGCGG CCGCTC(-3/-3) BsrI 239 CCAGT ACTGG(1/-1) CelII 282 GCTGAGC GC^T[ACGT]AGC CfoI 280 GCGC GCG^C DraI 162 TTTAAA TTT^AAA EaeI 291 CGGCCA [CT]^GGCC[GA] EarI 517 GAAGAG CTCTTC(1/4) FbaI 729 TGATCA T^GATCA HhaI 280 GCGC GCG^C HinP1I 280 GCGC G^CGC HindIII 778 AAGCTT A^AGCTT Ksp632I 517 GAAGAG CTCTTC(1/4) MboII 517 GAAGA GAAGA(8/7) MspA1I 538 CAGCTG C[AC]G^C[GT]G NaeI 297 GCCGGC GCC^GGC NgoMIV 297 GCCGGC G^CCGGC PpuMI 602 AGGACCT [GA]G^G[AT]CC[CT] PvuII 538 CAGCTG CAG^CTG RsaI 41 GTAC GT^AC SacI 520 GAGCTC GAGCT^C SapI 517 GAAGAGC GCTCTTC(1/4) SfaNI 706 GCATC GCATC(5/9) StuI 821 AGGCCT AGG^CCT TaqI 359 TCGA T^CGA Tsp509I 657 AATT ^AATT TthHB8I 359 TCGA T^CGA XcmI 797 CCAAAAAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### BanII 200 GGGCTC G[GA]GC[CT]^C 520 GAGCTC BfaI 86 CTAG C^TAG 868 CTAG BmyI 200 GGGCTC G[AGT]GC[ACT]^C 520 GAGCTC BpmI 628 CTGGAG CTGGAG(16/14) 838 CTGGAG Bsp1286I 200 GGGCTC G[AGT]GC[ACT]^C 520 GAGCTC DpnII 679 GATC ^GATC 730 GATC DraII 602 AGGACCT [GA]G^G[ACGT]CC[CT] 671 AGGGCCT EcoO109I 602 AGGACCT [GA]G^G[ACGT]CC[CT] 671 AGGGCCT HincII 553 GTCAAC GT[CT]^[GA]AC 576 GTTGAC HindII 553 GTCAAC GT[CT]^[GA]AC 576 GTTGAC HphI 694 GGTGA GGTGA(8/7) 413 TCACC MaeI 86 CTAG C^TAG 868 CTAG MboI 679 GATC ^GATC 730 GATC NdeII 679 GATC ^GATC 730 GATC NlaIII 47 CATG CATG^ 735 CATG NlaIV 6 GGCTCC GG[ACGT]^[ACGT]CC 201 GGCTCC PstI 149 CTGCAG CTGCA^G 265 CTGCAG Sau3AI 679 GATC ^GATC 730 GATC SmlI 523 CTCAAG C^T[CT][GA]AG 919 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AluI AvaII BbvI BsaJI BseRI BsiYI BslI BsmAI BsrFI BssKI BstNI Cac8I Cfr10I Cfr13I DdeI EcoRII FauI Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MnlI MseI MspI MvaI MwoI PleI Sau96I ScrFI SfcI TfiI Tru9I TseI TspRI