(Circular) (Six-base) MAPSORT of: pFN21AE9694.seq Check: 191 from: 1 to: 5653 pFN21AE9694 5653 bp With 182 enzymes: SgfI * December 8, 2014 13:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2060 2996 1937 Size: 123 936 4594 Fragments arranged by size: 4594 936 123 Acc65I G'GTAC_C Cuts at: 2974 2974 Size: 5653 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2624 2801 3716 5100 5368 1632 Size: 992 177 915 1384 268 1917 Fragments arranged by size: 1917 1384 992 915 268 177 AflII C'TTAA_G Cuts at: 820 1017 2924 820 Size: 197 1907 3549 Fragments arranged by size: 3549 1907 197 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2153 3396 4968 2153 Size: 1243 1572 2838 Fragments arranged by size: 2838 1572 1243 ApaLI G'TGCA_C Cuts at: 3710 4866 3710 Size: 1156 4497 ApoI r'AATT_y Cuts at: 1493 2962 3188 3340 5449 1493 Size: 1469 226 152 2109 1697 Fragments arranged by size: 2109 1697 1469 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2319 2978 1870 Size: 73 376 659 4545 Fragments arranged by size: 4545 659 376 73 BamHI G'GATC_C Cuts at: 2983 3350 2983 Size: 367 5286 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 4429 618 Size: 325 217 627 1187 1455 1842 Fragments arranged by size: 1842 1455 1187 627 325 217 BanII G_rGCy'C Cuts at: 1391 2209 2529 2972 1391 Size: 818 320 443 4072 Fragments arranged by size: 4072 818 443 320 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4682 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 2734 1548 Size: 1186 4467 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4264 136 Size: 107 122 71 631 605 337 2255 1525 Fragments arranged by size: 2255 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 2288 3070 2288 Size: 782 4871 Bme1580I G_kGCm'C Cuts at: 1790 3714 4870 1790 Size: 1924 1156 2573 Fragments arranged by size: 2573 1924 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 2864 4313 1239 Size: 1415 210 1449 2579 Fragments arranged by size: 2579 1449 1415 210 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2513 3778 4643 4941 2513 Size: 1265 865 298 3225 Fragments arranged by size: 3225 1265 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2696 4316 882 Size: 617 1197 1620 2219 Fragments arranged by size: 2219 1620 1197 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2097 4086 4484 4758 4905 5316 2097 Size: 1989 398 274 147 411 2434 Fragments arranged by size: 2434 1989 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5428 Fragments arranged by size: 5428 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2269 2789 2831 1186 Size: 1033 50 520 42 4008 Fragments arranged by size: 4008 1033 520 50 42 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2529 3714 3799 4870 2529 Size: 1185 85 1071 3312 Fragments arranged by size: 3312 1185 1071 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 BsmBI CGTCTCn'nnnn_ Cuts at: 2422 2422 Size: 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2209 2529 2972 3714 3799 4870 1391 Size: 399 419 320 443 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 419 399 320 85 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3008 844 Size: 2164 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2292 3540 1394 Size: 429 469 1248 3507 Fragments arranged by size: 3507 1248 469 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2135 2199 2302 4297 4484 1700 Size: 110 325 64 103 1995 187 2869 Fragments arranged by size: 2869 1995 325 187 110 103 64 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648 Size: 1011 367 385 17 1452 86 12 346 Cuts at: 5648 1972 Size: 1977 Fragments arranged by size: 1977 1452 1011 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4162 Fragments arranged by size: 4162 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2460 3169 3963 3983 2460 Size: 709 794 20 4130 Fragments arranged by size: 4130 794 709 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2169 2957 2169 Size: 788 4865 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2296 3100 3989 Size: 54 1425 50 275 18 466 804 889 Cuts at: 3989 5618 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 804 466 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2517 3583 1353 Size: 1164 1066 3423 Fragments arranged by size: 3423 1164 1066 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2076 4240 4614 4760 387 Size: 1064 138 487 2164 374 146 1280 Fragments arranged by size: 2164 1280 1064 487 374 146 138 EcoICRI GAG'CTC Cuts at: 2527 2527 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2624 2654 2801 2864 3716 4313 5100 Size: 393 992 30 147 63 852 597 787 Cuts at: 5100 5368 1239 Size: 268 1524 Fragments arranged by size: 1524 992 852 787 597 393 268 147 63 30 EcoO109I rG'GnC_Cy Cuts at: 2608 2677 2608 Size: 69 5584 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 5653 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 4800 1678 Size: 3122 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2575 2607 4294 4326 4368 Size: 32 135 32 1003 32 1687 32 42 Cuts at: 4368 4400 1373 Size: 32 2626 Fragments arranged by size: 2626 1687 1003 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2560 2583 2997 3243 669 Size: 988 281 622 23 414 246 3079 Fragments arranged by size: 3079 988 622 414 281 246 23 HindIII A'AGCT_T Cuts at: 748 2783 748 Size: 2035 3618 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2978 2978 Size: 5653 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4767 4951 1305 Size: 3462 184 2007 Fragments arranged by size: 3462 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3831 Fragments arranged by size: 3831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3611 3970 4129 518 Size: 648 2445 359 159 2042 Fragments arranged by size: 2445 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2545 3069 3746 4894 5139 1606 Size: 939 524 677 1148 245 2120 Fragments arranged by size: 2120 1148 939 677 524 245 NaeI GCC'GGC Cuts at: 2304 2304 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4162 Fragments arranged by size: 4162 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NgoMIV G'CCGG_C Cuts at: 2302 2302 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 5653 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2158 2274 3005 830 Size: 1328 116 731 3478 Fragments arranged by size: 3478 1328 731 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 PvuII CAG'CTG Cuts at: 2545 2545 Size: 5653 SacI G_AGCT'C Cuts at: 2529 2529 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2517 2517 Size: 5653 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2061 2154 2270 3001 4136 4817 5008 Size: 219 1016 93 116 731 1135 681 191 Cuts at: 5008 826 Size: 1471 Fragments arranged by size: 1471 1135 1016 731 681 219 191 116 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2528 2924 3757 4658 4920 820 Size: 197 926 585 396 833 901 262 1553 Fragments arranged by size: 1553 926 901 833 585 396 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 2828 1517 Size: 1311 4342 StyI C'CwwG_G Cuts at: 513 1065 2004 3092 513 Size: 552 939 1088 3074 Fragments arranged by size: 3074 1088 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2094 3680 3697 3850 4035 4480 325 Size: 1255 514 1586 17 153 185 445 1498 Fragments arranged by size: 1586 1498 1255 514 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3899 5611 96 Size: 274 80 33 51 1443 1922 1712 138 Fragments arranged by size: 1922 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5613 5645 5613 Size: 32 5621 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2809 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI