################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 709 GTCTAC GT^[AC][GT]AC AflIII 488 ACGTGT A^C[GA][CT]GT AlwI 255 GATCC GGATC(4/5) AlwNI 652 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 331 GGCACC G^G[CT][GA]CC BanII 220 GAGCTC G[GA]GC[CT]^C BbrPI 517 CACGTG CAC^GTG BbsI 532 GTCTTC GAAGAC(2/6) BclI 26 TGATCA T^GATCA BlpI 681 GCTGAGC GC^T[ACGT]AGC BpmI 691 CTGGAG CTGGAG(16/14) Bpu1102I 681 GCTGAGC GC^T[ACGT]AGC BpuAI 532 GTCTTC GAAGAC(2/6) BsaAI 517 CACGTG [CT]AC^GT[GA] BsrI 57 ACTGG ACTGG(1/-1) BssHII 664 GCGCGC G^CGCGC BstEII 385 GGTCACC G^GT[ACGT]ACC BstPI 385 GGTCACC G^GT[ACGT]ACC BstYI 254 AGATCC [GA]^GATC[CT] CelII 681 GCTGAGC GC^T[ACGT]AGC EcoO65I 385 GGTCACC G^GT[ACGT]ACC FbaI 26 TGATCA T^GATCA HincII 139 GTTGAC GT[CT]^[GA]AC HindII 139 GTTGAC GT[CT]^[GA]AC HinfI 325 GAGTC G^A[ACGT]TC MboII 533 TCTTC GAAGA(8/7) MflI 254 AGATCC [GA]^GATC[CT] NaeI 772 GCCGGC GCC^GGC NcoI 63 CCATGG C^CATGG NgoMIV 772 GCCGGC G^CCGGC NspI 646 ACATGC [GA]CATG^[CT] PflFI 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 651 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 325 GAGTC GAGTC(4/5) PmaCI 517 CACGTG CAC^GTG PmlI 517 CACGTG CAC^GTG SacI 220 GAGCTC GAGCT^C SfaNI 626 GCATC GCATC(5/9) SmaI 431 CCCGGG CCC^GGG SmlI 586 CTCAAG C^T[CT][GA]AG Tth111I 526 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 651 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 254 AGATCC [GA]^GATC[CT] XmaCI 431 CCCGGG C^CCGGG XmaI 431 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 665 CGCG CG^CG 667 CGCG AcyI 526 GACGCC G[GA]^CG[CT]C 580 GACGCC AvaI 217 CCCGAG C^[CT]CG[GA]G 431 CCCGGG AviII 476 TGCGCA TGC^GCA 719 TGCGCA BsaHI 526 GACGCC G[GA]^CG[CT]C 580 GACGCC BseRI 19 GAGGAG GAGGAG(10/8) 436 GAGGAG BsiEI 150 CGGCCG CG[GA][CT]^CG 180 CGGCCG BsmFI 578 GGGAC GGGAC(10/14) 351 GTCCC BsoBI 217 CCCGAG C^[CT]CG[GA]G 431 CCCGGG BsrDI 372 CATTGC GCAATG(2/0) 70 GCAATG BstUI 665 CGCG CG^CG 667 CGCG BstXI 287 CCATGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 547 CCATCACCCTGG DpnII 27 GATC ^GATC 255 GATC DraIII 394 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 451 CACCGTGTG EagI 150 CGGCCG C^GGCCG 180 CGGCCG EclXI 150 CGGCCG C^GGCCG 180 CGGCCG Eco52I 150 CGGCCG C^GGCCG 180 CGGCCG EcoT14I 63 CCATGG C^C[AT][AT]GG 167 CCAAGG FauI 417 GCGGG CCCGC(4/6) 599 GCGGG FspI 476 TGCGCA TGC^GCA 719 TGCGCA HaeII 602 GGCGCT [GA]GCGC^[CT] 654 GGCGCT Hin1I 526 GACGCC G[GA]^CG[CT]C 580 GACGCC MboI 27 GATC ^GATC 255 GATC MvnI 665 CGCG CG^CG 667 CGCG NdeII 27 GATC ^GATC 255 GATC Sau3AI 27 GATC ^GATC 255 GATC StyI 63 CCATGG C^C[AT][AT]GG 167 CCAAGG TaqI 593 TCGA T^CGA 629 TCGA TthHB8I 593 TCGA T^CGA 629 TCGA XcmI 47 CCATCCTGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 108 CCAGACCCGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AspHI BbvI BcnI BglI BmyI BsaJI BsgI BsiHKAI BsiYI BslI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MnlI MslI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI TseI Tsp45I TspRI