(Circular) (Six-base) MAPSORT of: pFN21AE1783.seq Check: 6805 from: 1 to: 5572 pFN21AE1783 5572 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2403 2629 2403 Size: 226 5346 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 2915 1937 Size: 778 200 4594 Fragments arranged by size: 4594 778 200 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3635 5019 5287 1632 Size: 2003 1384 268 1917 Fragments arranged by size: 2003 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 2493 4471 5448 1203 Size: 1290 1978 977 1327 Fragments arranged by size: 1978 1327 1290 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 2662 3315 4887 2662 Size: 653 1572 3347 Fragments arranged by size: 3347 1572 653 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2222 2436 2897 1870 Size: 73 279 214 461 4545 Fragments arranged by size: 4545 461 279 214 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2893 4348 618 Size: 325 217 627 549 557 1455 1842 Fragments arranged by size: 1842 1455 627 557 549 325 217 BanII G_rGCy'C Cuts at: 1391 2229 2891 1391 Size: 838 662 4072 Fragments arranged by size: 4072 838 662 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2530 928 Size: 971 631 3970 Fragments arranged by size: 3970 971 631 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 2031 1548 Size: 483 5089 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 2193 2505 Size: 107 122 71 631 605 494 27 312 Cuts at: 2505 2778 2838 4183 136 Size: 273 60 1345 1525 Fragments arranged by size: 1525 1345 631 605 494 312 273 122 107 71 60 27 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2687 2989 2687 Size: 302 5270 Bme1580I G_kGCm'C Cuts at: 1790 2107 2759 3633 4789 1790 Size: 317 652 874 1156 2573 Fragments arranged by size: 2573 1156 874 652 317 BmgBI CAC'GTC Cuts at: 1318 2181 2494 1318 Size: 863 313 4396 Fragments arranged by size: 4396 863 313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2072 3303 4261 Size: 416 53 83 399 24 655 1231 958 Cuts at: 4261 442 Size: 1753 Fragments arranged by size: 1753 1231 958 655 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2717 4232 1239 Size: 1478 1515 2579 Fragments arranged by size: 2579 1515 1478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2576 3697 4562 4860 2576 Size: 1121 865 298 3288 Fragments arranged by size: 3288 1121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4235 882 Size: 617 2736 2219 Fragments arranged by size: 2736 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2524 3363 493 Size: 711 1320 839 2702 Fragments arranged by size: 2702 1320 839 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2532 2586 3379 3761 Size: 53 83 186 936 999 54 793 382 Cuts at: 3761 4396 275 Size: 635 1451 Fragments arranged by size: 1451 999 936 793 635 382 186 83 54 53 BsaWI w'CCGG_w Cuts at: 4005 4403 4677 4824 5235 4005 Size: 398 274 147 411 4342 Fragments arranged by size: 4342 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5347 Fragments arranged by size: 5347 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2456 1186 Size: 853 417 4302 Fragments arranged by size: 4302 853 417 BseYI C'CCAG_C Cuts at: 2196 2469 4775 5271 2196 Size: 273 2306 496 2497 Fragments arranged by size: 2497 2306 496 273 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2114 2195 2381 1163 Size: 573 378 81 186 4354 Fragments arranged by size: 4354 573 378 186 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2158 2188 3022 3783 4811 1540 Size: 275 186 157 30 834 761 1028 2301 Fragments arranged by size: 2301 1028 834 761 275 186 157 30 BsiHKAI G_wGCw'C Cuts at: 2229 2457 2693 3633 3718 4789 2229 Size: 228 236 940 85 1071 3012 Fragments arranged by size: 3012 1071 940 236 228 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2229 2457 2693 2759 2891 3633 Size: 399 317 122 228 236 66 132 742 Cuts at: 3633 3718 4789 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 317 236 228 132 122 85 66 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2250 2403 2629 2927 844 Size: 1406 153 226 298 3489 Fragments arranged by size: 3489 1406 298 226 153 BsrBI CCG'CTC Cuts at: 1394 1823 3459 1394 Size: 429 1636 3507 Fragments arranged by size: 3507 1636 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2082 2376 4067 4241 65 Size: 1183 834 294 1691 174 1396 Fragments arranged by size: 1691 1396 1183 834 294 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2339 2777 2837 4216 4403 1700 Size: 110 529 438 60 1379 187 2869 Fragments arranged by size: 2869 1379 529 438 187 110 60 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 2669 1925 Size: 744 4828 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 2390 1184 Size: 1206 4366 BstXI CCAn_nnnn'nTGG Cuts at: 2299 2559 2299 Size: 260 5312 BstYI r'GATC_y Cuts at: 1972 2259 2902 3269 3654 3671 5123 5209 5221 Size: 287 643 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 643 385 367 346 287 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2068 2385 2568 2826 513 Size: 552 1003 317 183 258 3259 Fragments arranged by size: 3259 1003 552 317 258 183 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2263 487 Size: 683 56 1037 3796 Fragments arranged by size: 3796 1037 683 56 BtsI GCAGTG_nn' Cuts at: 2091 2350 2509 3088 3882 3902 2091 Size: 259 159 579 794 20 3761 Fragments arranged by size: 3761 794 579 259 159 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DraIII CAC_nnn'GTG Cuts at: 2404 2461 2404 Size: 57 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2155 2185 Size: 54 1425 50 275 18 219 106 30 Cuts at: 2185 2526 3019 3908 5537 8 Size: 341 493 889 1629 43 Fragments arranged by size: 1629 1425 889 493 341 275 219 106 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 2155 2185 3019 1537 Size: 275 343 30 834 4090 Fragments arranged by size: 4090 834 343 275 30 EarI CTCTTCn'nnn_ Cuts at: 1353 3502 1353 Size: 2149 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4159 4533 4679 387 Size: 1064 138 2570 374 146 1280 Fragments arranged by size: 2570 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2227 2227 Size: 5572 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2717 3635 4232 5019 5287 1239 Size: 393 1085 918 597 787 268 1524 Fragments arranged by size: 1524 1085 918 787 597 393 268 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 2483 2726 4078 2483 Size: 243 1352 3977 Fragments arranged by size: 3977 1352 243 FspAI rTGC'GCAy Cuts at: 2483 2726 2483 Size: 243 5329 HaeII r_GCGC'y Cuts at: 1678 2611 2663 4719 1678 Size: 933 52 2056 2531 Fragments arranged by size: 2531 2056 933 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2537 2569 4213 4245 4287 Size: 32 135 32 965 32 1644 32 42 Cuts at: 4287 4319 1373 Size: 32 2626 Fragments arranged by size: 2626 1644 965 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2916 3162 669 Size: 988 281 208 770 246 3079 Fragments arranged by size: 3079 988 770 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 2808 4686 4870 1305 Size: 975 528 1878 184 2007 Fragments arranged by size: 2007 1878 975 528 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3750 Fragments arranged by size: 3750 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2294 2813 3530 3889 4048 518 Size: 648 1125 3 519 717 359 159 2042 Fragments arranged by size: 2042 1125 717 648 519 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2422 2469 2805 2988 3665 4813 5058 1606 Size: 816 47 336 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 816 677 336 245 183 47 NaeI GCC'GGC Cuts at: 2779 2779 Size: 5572 NcoI C'CATG_G Cuts at: 513 1065 2068 513 Size: 552 1003 4017 Fragments arranged by size: 4017 1003 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NgoMIV G'CCGG_C Cuts at: 2777 2777 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2655 2930 4475 1132 Size: 97 1426 275 1545 2229 Fragments arranged by size: 2229 1545 1426 275 97 PasI CC'CwG_GG Cuts at: 1348 2559 1348 Size: 1211 4361 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 2662 1108 Size: 1554 4018 PfoI T'CCnGG_A Cuts at: 2448 2541 2448 Size: 93 5479 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PmlI CAC'GTG Cuts at: 2524 2524 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2924 830 Size: 2094 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 SacI G_AGCT'C Cuts at: 2229 2229 Size: 5572 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SfcI C'TryA_G Cuts at: 826 1045 2920 4055 4736 4927 826 Size: 219 1875 1135 681 191 1471 Fragments arranged by size: 1875 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5572 SmaI CCC'GGG Cuts at: 1872 2438 2899 1872 Size: 566 461 4545 Fragments arranged by size: 4545 566 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 2591 3676 4577 4839 820 Size: 197 926 648 1085 901 262 1553 Fragments arranged by size: 1553 1085 926 901 648 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5572 StyI C'CwwG_G Cuts at: 513 1065 2068 2172 3011 513 Size: 552 1003 104 839 3074 Fragments arranged by size: 3074 1003 839 552 104 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 2398 2461 2501 3599 3616 3769 Size: 1255 608 210 63 40 1098 17 153 Cuts at: 3769 3954 4399 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1098 608 445 210 185 153 63 40 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5532 5564 5532 Size: 32 5540 Tth111I GACn'n_nGTC Cuts at: 1316 2534 1316 Size: 1218 4354 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2059 2120 2011 Size: 48 61 5463 Fragments arranged by size: 5463 61 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2436 2897 1870 Size: 566 461 4545 Fragments arranged by size: 4545 566 461 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI