################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### Acc65I 274 GGTACC G^GTACC AccI 667 GTAGAC GT^[AC][GT]AC AccII 200 CGCG CG^CG AflIII 199 ACGCGT A^C[GA][CT]GT Alw44I 256 GTGCAC G^TGCAC AlwNI 884 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 256 GTGCAC G^TGCAC Asp718I 274 GGTACC G^GTACC BbrPI 679 CACGTG CAC^GTG BfaI 562 CTAG C^TAG BseRI 769 GAGGAG GAGGAG(10/8) BsmBI 892 GAGACG CGTCTC(1/5) BspMI 59 ACCTGC ACCTGC(4/8) BsrBI 462 CCGCTC CCGCTC(-3/-3) BsrDI 183 CATTGC GCAATG(2/0) BstUI 200 CGCG CG^CG BstXI 149 CCACCACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 620 GCGC GCG^C DraIII 710 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 478 CTCTTC CTCTTC(1/4) EcoT14I 123 CCATGG C^C[AT][AT]GG HhaI 620 GCGC GCG^C HinP1I 620 GCGC G^CGC KpnI 274 GGTACC GGTAC^C Ksp632I 478 CTCTTC CTCTTC(1/4) MaeI 562 CTAG C^TAG MluI 199 ACGCGT A^CGCGT MvnI 200 CGCG CG^CG NcoI 123 CCATGG C^CATGG NspI 702 GCATGT [GA]CATG^[CT] PmaCI 679 CACGTG CAC^GTG PmlI 679 CACGTG CAC^GTG SapI 477 GCTCTTC GCTCTTC(1/4) SbfI 507 CCTGCAGG CCTGCA^GG SexAI 307 ACCTGGT A^CC[AT]GGT SmaI 89 CCCGGG CCC^GGG Sse8387I 507 CCTGCAGG CCTGCA^GG StyI 123 CCATGG C^C[AT][AT]GG XmaCI 89 CCCGGG C^CCGGG XmaI 89 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AfaI 275 GTAC GT^AC 732 GTAC AvaI 65 CTCGGG C^[CT]CG[GA]G 89 CCCGGG AvaII 46 GGACC G^G[AT]CC 459 GGACC BalI 374 TGGCCA TGG^CCA 437 TGGCCA BpmI 870 CTCCAG CTGGAG(16/14) 754 CTGGAG BsaAI 679 CACGTG [CT]AC^GT[GA] 733 TACGTG BsoBI 65 CTCGGG C^[CT]CG[GA]G 89 CCCGGG EaeI 374 TGGCCA [CT]^GGCC[GA] 437 TGGCCA HinfI 179 GAGTC G^A[ACGT]TC 868 GACTC MluNI 374 TGGCCA TGG^CCA 437 TGGCCA MscI 374 TGGCCA TGG^CCA 437 TGGCCA MslI 249 CATCCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 640 CACCGTGATG PleI 868 GACTC GAGTC(4/5) 179 GAGTC PvuII 191 CAGCTG CAG^CTG 361 CAGCTG RsaI 275 GTAC GT^AC 732 GTAC SacI 475 GAGCTC GAGCT^C 832 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwI AspHI BanI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI XhoII