(Circular) (Six-base) MAPSORT of: pFN21AE1226.seq Check: 1420 from: 1 to: 5605 pFN21AE1226 5605 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2073 2073 Size: 5605 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2673 2948 1937 Size: 736 275 4594 Fragments arranged by size: 4594 736 275 Acc65I G'GTAC_C Cuts at: 2279 2926 2279 Size: 647 4958 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3668 5052 5320 1632 Size: 2036 1384 268 1917 Fragments arranged by size: 2036 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2204 4504 5481 1203 Size: 1001 2300 977 1327 Fragments arranged by size: 2300 1327 1001 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2528 1184 Size: 1344 4261 AlwNI CAG_nnn'CTG Cuts at: 2894 3348 4920 2894 Size: 454 1572 3579 Fragments arranged by size: 3579 1572 454 ApaLI G'TGCA_C Cuts at: 2261 3662 4818 2261 Size: 1401 1156 3048 Fragments arranged by size: 3048 1401 1156 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2094 2930 1870 Size: 73 127 24 836 4545 Fragments arranged by size: 4545 836 127 73 24 BamHI G'GATC_C Cuts at: 2935 3302 2935 Size: 367 5238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2279 2291 2504 2926 Size: 325 217 627 363 129 12 213 422 Cuts at: 2926 4381 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 422 363 325 217 213 129 12 BanII G_rGCy'C Cuts at: 1391 2484 2540 2669 2841 2924 1391 Size: 1093 56 129 172 83 4072 Fragments arranged by size: 4072 1093 172 129 83 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2094 2265 3666 4822 1790 Size: 304 171 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 304 171 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2367 3336 4294 Size: 416 53 83 399 24 950 969 958 Cuts at: 4294 442 Size: 1753 Fragments arranged by size: 1753 969 958 950 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2780 2860 4265 1239 Size: 1541 80 1405 2579 Fragments arranged by size: 2579 1541 1405 80 Bpu10I CC'TnA_GC Cuts at: 2478 2799 2478 Size: 321 5284 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4268 882 Size: 617 2769 2219 Fragments arranged by size: 2769 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2686 2740 3396 493 Size: 711 1482 54 656 2702 Fragments arranged by size: 2702 1482 711 656 54 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2696 2726 2739 2769 1178 Size: 30 165 30 1293 30 13 30 4014 Fragments arranged by size: 4014 1293 165 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 1186 Size: 1603 4002 BseYI C'CCAG_C Cuts at: 2082 2460 4808 5304 2082 Size: 378 2348 496 2383 Fragments arranged by size: 2383 2348 496 378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5032 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2265 2484 2597 2841 3666 3751 4822 2265 Size: 219 113 244 825 85 1071 3048 Fragments arranged by size: 3048 1071 825 244 219 113 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 BsmBI CGTCTCn'nnnn_ Cuts at: 2891 2891 Size: 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2265 2484 2540 2597 2669 2841 Size: 399 304 171 219 56 57 72 172 Cuts at: 2841 2924 3666 3751 4822 1391 Size: 83 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 219 172 171 85 83 72 57 56 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2073 2960 844 Size: 1229 887 3489 Fragments arranged by size: 3489 1229 887 BsrBI CCG'CTC Cuts at: 1394 1823 2469 3492 1394 Size: 429 646 1023 3507 Fragments arranged by size: 3507 1023 646 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2187 4100 4274 65 Size: 1183 939 1913 174 1396 Fragments arranged by size: 1913 1396 1183 939 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4249 4436 1700 Size: 110 2439 187 2869 Fragments arranged by size: 2869 2439 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2161 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2124 2431 2543 2935 3302 3687 3704 5156 Size: 152 307 112 392 367 385 17 1452 Cuts at: 5156 5242 5254 5600 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 392 385 367 346 307 152 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2128 2146 2572 2628 513 Size: 552 939 124 18 426 56 3490 Fragments arranged by size: 3490 939 552 426 124 56 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 2445 2725 2808 3121 3915 3935 2445 Size: 280 83 313 794 20 4115 Fragments arranged by size: 4115 794 313 280 83 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2720 2720 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2379 2442 3052 Size: 54 1425 50 275 18 549 63 610 Cuts at: 3052 3941 5570 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 610 549 275 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 3535 1353 Size: 1136 1046 3423 Fragments arranged by size: 3423 1136 1046 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 2839 2482 Size: 357 5248 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2780 2860 3668 4265 5052 5320 1239 Size: 393 1148 80 808 597 787 268 1524 Fragments arranged by size: 1524 1148 808 787 597 393 268 80 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2347 2379 2418 2450 2513 Size: 32 135 32 775 32 39 32 63 Cuts at: 2513 2545 2696 2728 4246 4278 4320 4352 1373 Size: 32 151 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 775 151 135 63 42 39 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2283 2930 2283 Size: 647 4958 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 2204 5481 2204 Size: 3277 2328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4719 4903 1305 Size: 3414 184 2007 Fragments arranged by size: 3414 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2381 2444 10 Size: 54 1425 343 549 63 3171 Fragments arranged by size: 3171 1425 549 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2649 3563 3922 4081 518 Size: 648 1092 391 914 359 159 2042 Fragments arranged by size: 2042 1092 914 648 391 359 159 MspA1I CmG'CkG Cuts at: 1606 2198 2332 2368 3021 3698 4846 5091 1606 Size: 592 134 36 653 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 653 592 245 134 36 NcoI C'CATG_G Cuts at: 513 1065 2004 2128 513 Size: 552 939 124 3990 Fragments arranged by size: 3990 939 552 124 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2711 2963 4508 1132 Size: 97 1482 252 1545 2229 Fragments arranged by size: 2229 1545 1482 252 97 PasI CC'CwG_GG Cuts at: 1348 2047 1348 Size: 699 4906 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5605 PfoI T'CCnGG_A Cuts at: 2757 2876 2757 Size: 119 5486 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PmlI CAC'GTG Cuts at: 2686 2686 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2016 2124 2197 2517 2827 2957 830 Size: 1186 108 73 320 310 130 3478 Fragments arranged by size: 3478 1186 320 310 130 108 73 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 PvuII CAG'CTG Cuts at: 2198 2368 2198 Size: 170 5435 SacI G_AGCT'C Cuts at: 2484 2841 2484 Size: 357 5248 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SapI GCTCTTCn'nnn_ Cuts at: 2489 2489 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2517 2957 2517 Size: 440 5165 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SexAI A'CCwGG_T Cuts at: 2312 2312 Size: 5605 SfcI C'TryA_G Cuts at: 826 1045 2012 2120 2193 2494 2513 2670 2823 Size: 219 967 108 73 301 19 157 153 Cuts at: 2823 2953 4088 4769 4960 826 Size: 130 1135 681 191 1471 Fragments arranged by size: 1471 1135 967 681 301 219 191 157 153 130 108 73 19 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5605 SmaI CCC'GGG Cuts at: 1872 2096 2932 1872 Size: 224 836 4545 Fragments arranged by size: 4545 836 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2004 2128 3044 513 Size: 552 939 124 916 3074 Fragments arranged by size: 3074 939 916 552 124 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2612 2657 3632 3649 3802 3987 Size: 1255 480 552 45 975 17 153 185 Cuts at: 3987 4432 325 Size: 445 1498 Fragments arranged by size: 1498 1255 975 552 480 445 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2094 2930 1870 Size: 224 836 4545 Fragments arranged by size: 4545 836 224 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI