################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I CelII CfoI ClaI CpoI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeIII MamI MboII MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SpeI Sse8387I SspI StuI SwaI TaqI Tsp45I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AciI 239 CCGC CCGC(-3/-1) AcsI 119 AAATTC [GA]^AATT[CT] AluI 329 AGCT AG^CT AlwI 69 GATCC GGATC(4/5) AocI 335 CCTCAGG CC^T[ACGT]AGG ApaI 304 GGGCCC GGGCC^C ApoI 119 AAATTC [GA]^AATT[CT] AspI 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 3 GGCACC G^G[CT][GA]CC BanII 304 GGGCCC G[GA]GC[CT]^C BciVI 345 GTATCC GTATCC(6/5) BmyI 304 GGGCCC G[AGT]GC[ACT]^C BpmI 228 CTCCAG CTGGAG(16/14) BsaAI 388 TACGTA [CT]AC^GT[GA] BsgI 214 CTGCAC GTGCAG(16/14) BsiYI 411 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 411 CCAGAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 312 GTCCC GGGAC(10/14) Bsp1286I 304 GGGCCC G[AGT]GC[ACT]^C Bsp1407I 447 TGTACA T^GTACA BsrFI 6 ACCGGC [GA]^CCGG[CT] BsrGI 447 TGTACA T^GTACA BssKI 438 CCTGG ^CC[ACGT]GG BssSI 217 CACGAG CACGAG(-5/-1) BstNI 438 CCTGG CC^[AT]GG BstYI 68 AGATCC [GA]^GATC[CT] Bsu36I 335 CCTCAGG CC^T[ACGT]AGG Cfr10I 6 ACCGGC [GA]^CCGG[CT] DpnII 69 GATC ^GATC DraI 115 TTTAAA TTT^AAA DsaI 300 CCACGG C^C[GA][CT]GG Eco81I 335 CCTCAGG CC^T[ACGT]AGG EcoRII 438 CCTGG ^CC[AT]GG EcoT14I 89 CCAAGG C^C[AT][AT]GG HapII 7 CCGG C^CGG HpaII 7 CCGG C^CGG HphI 332 TCACC GGTGA(8/7) MaeII 389 ACGT A^CGT MboI 69 GATC ^GATC MflI 68 AGATCC [GA]^GATC[CT] MslI 104 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 7 CCGG C^CGG MvaI 438 CCTGG CC^[AT]GG NdeII 69 GATC ^GATC PflFI 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 354 CTGCAG CTGCA^G Sau3AI 69 GATC ^GATC ScrFI 438 CCTGG CC^[ACGT]GG SexAI 437 ACCTGGT A^CC[AT]GGT SfcI 354 CTGCAG C^T[GA][CT]AG SmlI 143 CTCAAG C^T[CT][GA]AG SnaBI 388 TACGTA TAC^GTA SphI 148 GCATGC GCATG^C SspBI 447 TGTACA T^GTACA StyI 89 CCAAGG C^C[AT][AT]GG TfiI 20 GATTC G^A[AT]TC Tth111I 457 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 68 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 360 GTAC GT^AC 448 GTAC BsaJI 89 CCAAGG C^C[ACGT][ACGT]GG 300 CCACGG BspMI 244 ACCTGC ACCTGC(4/8) 356 GCAGGT BsrI 73 CCAGT ACTGG(1/-1) 230 CCAGT Cac8I 148 GCATGC GC[ACGT]^[ACGT]GC 265 GCCTGC Cfr13I 304 GGGCC G^G[ACGT]CC 305 GGCCC EaeI 8 CGGCCA [CT]^GGCC[GA] 236 TGGCCG MseI 116 TTAA T^TAA 173 TTAA NspI 128 ACATGC [GA]CATG^[CT] 148 GCATGC PleI 421 GACTC GAGTC(4/5) 461 GAGTC RsaI 360 GTAC GT^AC 448 GTAC Sau96I 304 GGGCC G^G[ACGT]CC 305 GGCCC Tru9I 116 TTAA T^TAA 173 TTAA Tsp509I 120 AATT ^AATT 431 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### BbvI DdeI Fnu4HI HaeIII HinfI ItaI MnlI MwoI NlaIII NlaIV TseI TspRI