##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BcgI        BciVI       BclI        
BcnI        BfrI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstNI       BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvaI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        ScrFI       
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AciI          363  CCGC                 CCGC(-3/-1)
AlwI           44  GATCC                GGATC(4/5)
BbsI          313  GTCTTC               GAAGAC(2/6)
BbvCI         265  CCTCAGC              CCTCAGC(-5/-2)
BfaI          376  CTAG                 C^TAG
BglI          343  GCCTATGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI           31  CTCCAG               CTGGAG(16/14)
BpuAI         313  GTCTTC               GAAGAC(2/6)
BsaI          252  GGTCTC               GGTCTC(1/5)
BsaJI         247  CCTTGG               C^C[ACGT][ACGT]GG
BsmBI         157  GAGACG               CGTCTC(1/5)
BsmI           92  GCATTC               GAATGC(1/-1)
BspMI         391  ACCTGC               ACCTGC(4/8)
BsrI          417  CCAGT                ACTGG(1/-1)
BstF5I        178  GGATG                GGATG(2/0)
BstYI          43  AGATCC               [GA]^GATC[CT]
Cac8I         365  GCAGGC               GC[ACGT]^[ACGT]GC
EcoT14I       247  CCTTGG               C^C[AT][AT]GG
FokI          178  GGATG                GGATG(9/13)
HaeIII        351  GGCC                 GG^CC
HinfI          25  GATTC                G^A[ACGT]TC
MaeI          376  CTAG                 C^TAG
MaeIII        163  GTAAC                ^GT[ACGT]AC
MflI           43  AGATCC               [GA]^GATC[CT]
StuI          350  AGGCCT               AGG^CCT
StyI          247  CCTTGG               C^C[AT][AT]GG
TfiI           25  GATTC                G^A[AT]TC
XbaI          375  TCTAGA               T^CTAGA
XcmI           47  CCAGATATACATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          43  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AvaII         360  GGACC                G^G[AT]CC
              389  GGACC               
BbvI          296  GCAGC                GCAGC(8/12)
              369  GCAGC               
Cfr13I        360  GGACC                G^G[ACGT]CC
              389  GGACC               
DpnII          44  GATC                 ^GATC
              236  GATC                
EarI          152  GAAGAG               CTCTTC(1/4)
              183  GAAGAG              
Fnu4HI        296  GCAGC                GC^[ACGT]GC
              369  GCAGC               
ItaI          296  GCAGC                GC^[ACGT]GC
              369  GCAGC               
Ksp632I       152  GAAGAG               CTCTTC(1/4)
              183  GAAGAG              
MboI           44  GATC                 ^GATC
              236  GATC                
MwoI            3  GCTGGAAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              343  GCCTATGAGGC         
NdeII          44  GATC                 ^GATC
              236  GATC                
Sau3AI         44  GATC                 ^GATC
              236  GATC                
Sau96I        360  GGACC                G^G[ACGT]CC
              389  GGACC               
TseI          296  GCAGC                G^C[AT]GC
              369  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    6 enzymes) #
#########################################

AluI        BsmAI       DdeI        MboII       MnlI        
NlaIII