################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SalI SbfI ScaI SexAI SfaNI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AcsI 285 GAATTC [GA]^AATT[CT] AhdI 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 285 GAATTC [GA]^AATT[CT] Asp700I 255 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 163 GAGCAC G[AT]GC[AT]^C AvaII 78 GGACC G^G[AT]CC BcnI 51 CCCGG CC^[GC]GG BfaI 367 CTAG C^TAG BglI 34 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 163 GAGCAC G[AGT]GC[ACT]^C BpmI 169 CTGGAG CTGGAG(16/14) BsgI 236 GTGCAG GTGCAG(16/14) BsiHKAI 163 GAGCAC G[AT]GC[AT]^C BsmAI 353 GAGAC GTCTC(1/5) Bsp1286I 163 GAGCAC G[AGT]GC[ACT]^C BsrDI 378 CATTGC GCAATG(2/0) BssSI 139 CACGAG CACGAG(-5/-1) DdeI 335 CTCAG C^T[ACGT]AG DpnII 385 GATC ^GATC DraII 77 AGGACCT [GA]G^G[ACGT]CC[CT] Eam1105I 121 GACTTCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 311 CTCTTC CTCTTC(1/4) EcoO109I 77 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 285 GAATTC G^AATTC HapII 52 CCGG C^CGG HpaII 52 CCGG C^CGG Ksp632I 311 CTCTTC CTCTTC(1/4) MaeI 367 CTAG C^TAG MaeIII 328 GTCAC ^GT[ACGT]AC MboI 385 GATC ^GATC MboII 312 TCTTC GAAGA(8/7) MspI 52 CCGG C^CGG NciI 51 CCCGG CC^[GC]GG NdeII 385 GATC ^GATC NlaIII 177 CATG CATG^ PpuMI 77 AGGACCT [GA]G^G[AT]CC[CT] SapI 310 GCTCTTC GCTCTTC(1/4) Sau3AI 385 GATC ^GATC SfiI 33 GGCCGCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TfiI 241 GATTC G^A[AT]TC Tsp45I 328 GTCAC ^GT[GC]AC XmnI 255 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### BseRI 332 CTCCTC GAGGAG(10/8) 160 GAGGAG BstNI 57 CCTGG CC^[AT]GG 168 CCTGG Cfr13I 54 GGCCC G^G[ACGT]CC 78 GGACC EcoRII 57 CCTGG ^CC[AT]GG 168 CCTGG FauI 10 CCCGC CCCGC(4/6) 28 CCCGC HaeII 143 AGCGCC [GA]GCGC^[CT] 193 AGCGCC MvaI 57 CCTGG CC^[AT]GG 168 CCTGG Sau96I 54 GGCCC G^G[ACGT]CC 78 GGACC Tsp509I 286 AATT ^AATT 347 AATT TspRI 270 CAGTG CA[GC]TG[ACGT][ACGT]^ 324 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AccII AciI AluI BbvI BsaJI BsiEI BsiYI BslI BssKI BstUI Cac8I CfoI DsaI EaeI EagI EclXI Eco52I Fnu4HI HaeIII HgaI HhaI HinP1I HinfI ItaI KspI MnlI MspA1I MvnI MwoI NotI PleI SacII ScrFI TseI