Restriction map for
pFN21AE1624
(Circular) (Six-base) MAPSORT of: pFN21AE1624.seq Check: 8918 from: 1 to: 5116
pFN21AE1624 5116 bp
With 182 enzymes: SgfI *
March 28, 2012 12:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2926 278
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2459 1937
Size: 522 4594
Acc65I G'GTAC_C
Cuts at: 2437 2437
Size: 5116
AclI AA'CG_TT
Cuts at: 1563 3243 3616 1563
Size: 1680 373 3063
Fragments arranged by size:
3063 1680 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2209 2251 2257 3179 4563 4831 1632
Size: 577 42 6 922 1384 268 1917
Fragments arranged by size:
1917 1384 922 577 268 42 6
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4919
AflIII A'CryG_T
Cuts at: 1203 4015 4992 1203
Size: 2812 977 1327
Fragments arranged by size:
2812 1327 977
AgeI A'CCGG_T
Cuts at: 3947 3947
Size: 5116
AhdI GACnn_n'nnGTC
Cuts at: 2131 3845 2131
Size: 1714 3402
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5116
AlwNI CAG_nnn'CTG
Cuts at: 2859 4431 2859
Size: 1572 3544
ApaLI G'TGCA_C
Cuts at: 3173 4329 3173
Size: 1156 3960
ApoI r'AATT_y
Cuts at: 1493 2290 2425 2651 2803 4912 1493
Size: 797 135 226 152 2109 1697
Fragments arranged by size:
2109 1697 797 226 152 135
AseI AT'TA_AT
Cuts at: 160 1032 3670 160
Size: 872 2638 1606
Fragments arranged by size:
2638 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
AvaI C'yCGr_G
Cuts at: 1870 1943 2441 1870
Size: 73 498 4545
Fragments arranged by size:
4545 498 73
BamHI G'GATC_C
Cuts at: 2446 2813 2446
Size: 367 4749
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 3892 618
Size: 325 217 627 650 1455 1842
Fragments arranged by size:
1842 1455 650 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2435 1391
Size: 1044 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3307 3341 1903
Size: 34 1370 34 3678
Fragments arranged by size:
3678 1370 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3008 4218 3008
Size: 1210 3906
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5116
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 3727 136
Size: 107 122 71 631 605 373 1682 1525
Fragments arranged by size:
1682 1525 631 605 373 122 107 71
BglII A'GATC_T
Cuts at: 5111 5111
Size: 5116
BlpI GC'TnA_GC
Cuts at: 2533 2533
Size: 5116
Bme1580I G_kGCm'C
Cuts at: 1790 3177 4333 1790
Size: 1387 1156 2573
Fragments arranged by size:
2573 1387 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5116
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2847 3805 442
Size: 416 53 83 399 24 1430 958 1753
Fragments arranged by size:
1753 1430 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5116
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2195 3776 1239
Size: 956 1581 2579
Fragments arranged by size:
2579 1581 956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3241 4106 4404 3241
Size: 865 298 3953
Fragments arranged by size:
3953 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3779 882
Size: 617 2280 2219
Fragments arranged by size:
2280 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2907 493
Size: 711 1703 2702
Fragments arranged by size:
2702 1703 711
BsaBI GATnn'nnATC
Cuts at: 5110 5110
Size: 5116
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2923 3305 3940 275
Size: 53 83 186 936 1390 382 635 1451
Fragments arranged by size:
1451 1390 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3549 3947 4221 4368 4779 3549
Size: 398 274 147 411 3886
Fragments arranged by size:
3886 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2072 2102 2320 2350 1178
Size: 30 165 30 669 30 218 30 3944
Fragments arranged by size:
3944 669 218 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2180 2328 1186
Size: 994 148 3974
Fragments arranged by size:
3974 994 148
BseYI C'CCAG_C
Cuts at: 2311 4319 4815 2311
Size: 2008 496 2612
Fragments arranged by size:
2612 2008 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2262 1163
Size: 573 526 4017
Fragments arranged by size:
4017 573 526
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2040 2049 2076 2566 3327 4355
Size: 275 186 39 9 27 490 761 1028
Cuts at: 4355 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 490 275 186 39 27
9
BsiHKAI G_wGCw'C
Cuts at: 2172 3177 3262 4333 2172
Size: 1005 85 1071 2955
Fragments arranged by size:
2955 1071 1005 85
BsmI GAATG_Cn'
Cuts at: 2627 2720 2627
Size: 93 5023
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2172 2435 3177 3262 4333 1391
Size: 399 382 263 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 382 263 85
BspEI T'CCGG_A
Cuts at: 4779 4779
Size: 5116
BspHI T'CATG_A
Cuts at: 3005 4735 3005
Size: 1730 3386
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2471 844
Size: 1627 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 1394
Size: 429 1180 3507
Fragments arranged by size:
3507 1180 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2382 3611 3785 65
Size: 1183 1134 1229 174 1396
Fragments arranged by size:
1396 1229 1183 1134 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3760 3947 1700
Size: 110 1950 187 2869
Fragments arranged by size:
2869 1950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5116
BssSI C'ACGA_G
Cuts at: 2144 3176 4188 2144
Size: 1032 1012 3072
Fragments arranged by size:
3072 1032 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5116
BstBI TT'CG_AA
Cuts at: 3920 3920
Size: 5116
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5116
BstYI r'GATC_y
Cuts at: 1972 2446 2813 3198 3215 4667 4753 4765 5111
Size: 474 367 385 17 1452 86 12 346
Cuts at: 5111 1972
Size: 1977
Fragments arranged by size:
1977 1452 474 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2016 2034 2043 2076 513
Size: 552 951 18 9 33 3553
Fragments arranged by size:
3553 951 552 33 18 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4377
Fragments arranged by size:
4377 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 3426 3446 2632
Size: 794 20 4302
Fragments arranged by size:
4302 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5081
DraI TTT'AAA
Cuts at: 2420 2420
Size: 5116
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4123 809
Size: 507 2807 1802
Fragments arranged by size:
2807 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2046 2073
Size: 54 1425 50 275 18 207 9 27
Cuts at: 2073 2121 2563 3452 5081 8
Size: 48 442 889 1629 43
Fragments arranged by size:
1629 1425 889 442 275 207 54 50
48 43 27 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2037 2046 2073 2563 1537
Size: 275 225 9 27 490 4090
Fragments arranged by size:
4090 490 275 225 27 9
EarI CTCTTCn'nnn_
Cuts at: 1353 2322 3046 1353
Size: 969 724 3423
Fragments arranged by size:
3423 969 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3703 4077 4223 387
Size: 1064 138 2114 374 146 1280
Fragments arranged by size:
2114 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2209 2251 2257 3179 3776 4563
Size: 393 563 14 42 6 922 597 787
Cuts at: 4563 4831 1239
Size: 268 1524
Fragments arranged by size:
1524 922 787 597 563 393 268 42
14 6
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 5116
EcoRI G'AATT_C
Cuts at: 2290 2425 2803 2290
Size: 135 378 4603
Fragments arranged by size:
4603 378 135
FspI TGC'GCA
Cuts at: 3622 3622
Size: 5116
HaeII r_GCGC'y
Cuts at: 1678 2152 2202 4263 1678
Size: 474 50 2061 2531
Fragments arranged by size:
2531 2061 474 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2072 2104 2117 2149 3757
Size: 32 135 32 500 32 13 32 1608
Cuts at: 3757 3789 3831 3863 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1608 500 135 42 32 32 32
32 32 32 13
HincII GTy'rAC
Cuts at: 669 1657 1938 2460 2706 669
Size: 988 281 522 246 3079
Fragments arranged by size:
3079 988 522 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5116
HpaI GTT'AAC
Cuts at: 2706 2706
Size: 5116
KpnI G_GTAC'C
Cuts at: 2441 2441
Size: 5116
MfeI C'AATT_G
Cuts at: 2715 2715
Size: 5116
MluI A'CGCG_T
Cuts at: 4992 4992
Size: 5116
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2070 2321 4230 4414 1305
Size: 765 251 1909 184 2007
Fragments arranged by size:
2007 1909 765 251 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3294
Fragments arranged by size:
3294 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3074 3433 3592 518
Size: 648 1908 359 159 2042
Fragments arranged by size:
2042 1908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2018 2036 2045 2078 2220 2532 3209 4357
Size: 412 18 9 33 142 312 677 1148
Cuts at: 4357 4602 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 412 312 245 142 33
18 9
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4564
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5116
NotI GC'GGCC_GC
Cuts at: 2037 2046 2073 2563 2037
Size: 9 27 490 4590
Fragments arranged by size:
4590 490 27 9
NspI r_CATG'y
Cuts at: 1132 1229 2474 4019 1132
Size: 97 1245 1545 2229
Fragments arranged by size:
2229 1545 1245 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5116
PciI A'CATG_T
Cuts at: 4015 4015
Size: 5116
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5116
PmeI GTTT'AAAC
Cuts at: 2420 2420
Size: 5116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 4698 4730 3185
Size: 32 1481 32 3571
Fragments arranged by size:
3571 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 5116
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5116
PsiI TTA'TAA
Cuts at: 2686 2686
Size: 5116
PstI C_TGCA'G
Cuts at: 830 2468 830
Size: 1638 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5116
SacII CC_GC'GG
Cuts at: 2019 2037 2046 2079 2019
Size: 18 9 33 5056
Fragments arranged by size:
5056 33 18 9
SalI G'TCGA_C
Cuts at: 1936 2458 1936
Size: 522 4594
SapI GCTCTTCn'nnn_
Cuts at: 2322 2322
Size: 5116
SbfI CC_TGCA'GG
Cuts at: 2468 2468
Size: 5116
ScaI AGT'ACT
Cuts at: 3364 5076 3364
Size: 1712 3404
SfcI C'TryA_G
Cuts at: 826 1045 2464 3599 4280 4471 826
Size: 219 1419 1135 681 191 1471
Fragments arranged by size:
1471 1419 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2045 2045
Size: 5116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5116
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5116
SmaI CCC'GGG
Cuts at: 1872 2443 1872
Size: 571 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3220 4121 4383 820
Size: 197 926 1277 901 262 1553
Fragments arranged by size:
1553 1277 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2907 493
Size: 2414 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5116
SphI G_CATG'C
Cuts at: 1132 2474 1132
Size: 1342 3774
SspI AAT'ATT
Cuts at: 5 52 3040 5
Size: 47 2988 2081
Fragments arranged by size:
2988 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5116
StyI C'CwwG_G
Cuts at: 513 1065 2555 513
Size: 552 1490 3074
Fragments arranged by size:
3074 1490 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3160 3313 3498 3943 325
Size: 1255 1563 17 153 185 445 1498
Fragments arranged by size:
1563 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3362 5074 96
Size: 274 80 33 51 1443 1385 1712 138
Fragments arranged by size:
1712 1443 1385 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 2320 2352 5076 5108 2084
Size: 32 204 32 2724 32 2092
Fragments arranged by size:
2724 2092 204 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5116
XbaI T'CTAG_A
Cuts at: 2452 2452
Size: 5116
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5116
XmaI C'CCGG_G
Cuts at: 1870 2441 1870
Size: 571 4545
XmnI GAAnn'nnTTC
Cuts at: 2264 3245 2264
Size: 981 4135
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2924 276
Size: 53 83 186 936 1390 2468
Fragments arranged by size:
2468 1390 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI
SexAI SfoI SrfI SwaI XcmI