Restriction map for
pFN21AE6746
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# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AgeI Alw44I Aor51HI
ApaLI AscI Asp718I AspI AviII
AvrII BbvCI BcgI BlnI BlpI
Bpu1102I BsiWI BspLU11I BsrFI BssHII
BstXI CelII Cfr10I CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRV EcoT22I FseI FspI
KpnI MluI NaeI NgoMIV NheI
NotI NruI NsiI PacI PflFI
PinAI PmeI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 1158 GTATAC GT^[AC][GT]AC
AccIII 2330 TCCGGA T^CCGGA
AclI 2726 AACGTT AA^CGTT
AflII 31 CTTAAG C^TTAAG
AflIII 679 ACACGT A^C[GA][CT]GT
AhdI 2967 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 586 CCTCAGG CC^T[ACGT]AGG
AseI 2137 ATTAAT AT^TAAT
AsnI 2137 ATTAAT AT^TAAT
Asp700I 1742 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2967 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 59 GGATCC G^GATCC
BbeI 2464 GGCGCC GGCGC^C
BciVI 1078 GGATAC GTATCC(6/5)
BfaI 1709 CTAG C^TAG
BfrI 31 CTTAAG C^TTAAG
BglI 2212 GCCACAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 803 AGATCT A^GATCT
BsaWI 2330 TCCGGA [AT]^CCGG[AT]
BseAI 2330 TCCGGA T^CCGGA
BsiEI 3390 CGACCG CG[GA][CT]^CG
BsmI 1487 GCATTC GAATGC(1/-1)
Bsp1407I 2744 TGTACA T^GTACA
BspDI 2290 ATCGAT AT^CGAT
BspEI 2330 TCCGGA T^CCGGA
BspHI 1310 TCATGA T^CATGA
BsrBI 63 CCGCTC CCGCTC(-3/-3)
BsrGI 2744 TGTACA T^GTACA
BssSI 2358 CTCGTG CACGAG(-5/-1)
Bst1107I 1158 GTATAC GTA^TAC
BstAPI 433 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2622 GGTGACC G^GT[ACGT]ACC
BstPI 2622 GGTGACC G^GT[ACGT]ACC
BstZ17I 1158 GTATAC GTA^TAC
Bsu36I 586 CCTCAGG CC^T[ACGT]AGG
ClaI 2290 ATCGAT AT^CGAT
Eam1105I 2967 GACAAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 586 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2622 GGTGACC G^GT[ACGT]ACC
EcoRI 891 GAATTC G^AATTC
HindIII 2584 AAGCTT A^AGCTT
HpaI 445 GTTAAC GTT^AAC
KasI 2464 GGCGCC G^GCGCC
KspI 46 CCGCGG CCGC^GG
MaeI 1709 CTAG C^TAG
MfeI 386 CAATTG C^AATTG
MroI 2330 TCCGGA T^CCGGA
MunI 386 CAATTG C^AATTG
NarI 2464 GGCGCC GG^CGCC
NcoI 1223 CCATGG C^CATGG
PaeR7I 3332 CTCGAG C^TCGAG
PpuMI 75 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 1390 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2137 ATTAAT AT^TAAT
Psp1406I 2726 AACGTT AA^CGTT
PvuII 148 CAGCTG CAG^CTG
RcaI 1310 TCATGA T^CATGA
SacII 46 CCGCGG CCGC^GG
SfoI 2464 GGCGCC GGC^GCC
SmaI 125 CCCGGG CCC^GGG
SspBI 2744 TGTACA T^GTACA
SspI 3004 AATATT AAT^ATT
XhoI 3332 CTCGAG C^TCGAG
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
XmnI 1742 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AlwNI 946 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
3313 CAGGAGCTG
ApaI 724 GGGCCC GGGCC^C
1001 GGGCCC
AvaI 125 CCCGGG C^[CT]CG[GA]G
3332 CTCGAG
BalI 1226 TGGCCA TGG^CCA
2295 TGGCCA
BbrPI 680 CACGTG CAC^GTG
2497 CACGTG
BsaAI 680 CACGTG [CT]AC^GT[GA]
2497 CACGTG
BsaBI 1465 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2159 GATTGGTATC
BsmBI 628 GAGACG CGTCTC(1/5)
2459 GAGACG
BsoBI 125 CCCGGG C^[CT]CG[GA]G
3332 CTCGAG
BspMI 3283 ACCTGC ACCTGC(4/8)
1260 GCAGGT
BstBI 1739 TTCGAA TT^CGAA
2323 TTCGAA
EcoNI 2187 CCTATTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3450 CCTGCAGCAGG
HaeII 903 AGCGCC [GA]GCGC^[CT]
2464 GGCGCC
MamI 1465 GATCGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2159 GATTGGTATC
MluNI 1226 TGGCCA TGG^CCA
2295 TGGCCA
MscI 1226 TGGCCA TGG^CCA
2295 TGGCCA
MspA1I 46 CCGCGG C[AC]G^C[GT]G
148 CAGCTG
NdeI 2373 CATATG CA^TATG
3017 CATATG
NspV 1739 TTCGAA TT^CGAA
2323 TTCGAA
PflMI 491 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3059 CCACAGCTTGG
PmaCI 680 CACGTG CAC^GTG
2497 CACGTG
PmlI 680 CACGTG CAC^GTG
2497 CACGTG
SapI 278 GAAGAGC GCTCTTC(1/4)
1249 GAAGAGC
SfuI 1739 TTCGAA TT^CGAA
2323 TTCGAA
StuI 15 AGGCCT AGG^CCT
1565 AGGCCT
Van91I 491 CCATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3059 CCACAGCTTGG
XcmI 1124 CCATCAGTCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2936 CCATGAAAGCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanI BanII BbsI BbvI BclI
BcnI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII