(Linear) (Six-base) MAPSORT of: KIEE6746.seq Check: 9224 from: 1 to: 3564 KIEE6746.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1251 3292 3564 Size: 1251 2041 272 Fragments arranged by size: 2041 1251 272 AccI GT'mk_AC Cuts at: 0 1159 3564 Size: 1159 2405 AclI AA'CG_TT Cuts at: 0 2727 3564 Size: 2727 837 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1367 1733 2326 3564 Size: 1367 366 593 1238 Fragments arranged by size: 1367 1238 593 366 AflII C'TTAA_G Cuts at: 0 31 3564 Size: 31 3533 AflIII A'CryG_T Cuts at: 0 679 3564 Size: 679 2885 AhdI GACnn_n'nnGTC Cuts at: 0 2972 3564 Size: 2972 592 AleI CACnn'nnGTG Cuts at: 0 361 2358 2501 3564 Size: 361 1997 143 1063 Fragments arranged by size: 1997 1063 361 143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2376 3466 3491 3564 Size: 2376 1090 25 73 Fragments arranged by size: 2376 1090 73 25 AlwNI CAG_nnn'CTG Cuts at: 0 951 3318 3564 Size: 951 2367 246 Fragments arranged by size: 2367 951 246 ApaI G_GGCC'C Cuts at: 0 728 1005 3564 Size: 728 277 2559 Fragments arranged by size: 2559 728 277 ApoI r'AATT_y Cuts at: 0 199 323 891 1702 2007 2253 3073 3564 Size: 199 124 568 811 305 246 820 491 Fragments arranged by size: 820 811 568 491 305 246 199 124 AseI AT'TA_AT Cuts at: 0 2138 3564 Size: 2138 1426 AvaI C'yCGr_G Cuts at: 0 125 3332 3564 Size: 125 3207 232 Fragments arranged by size: 3207 232 125 BamHI G'GATC_C Cuts at: 0 59 3564 Size: 59 3505 BanI G'GyrC_C Cuts at: 0 156 363 2464 3564 Size: 156 207 2101 1100 Fragments arranged by size: 2101 1100 207 156 BanII G_rGCy'C Cuts at: 0 728 994 1005 1971 3202 3564 Size: 728 266 11 966 1231 362 Fragments arranged by size: 1231 966 728 362 266 11 BbeI G_GCGC'C Cuts at: 0 2468 3564 Size: 2468 1096 BbsI GAAGACnn'nnnn_ Cuts at: 0 1373 1614 3424 3564 Size: 1373 241 1810 140 Fragments arranged by size: 1810 1373 241 140 BciVI GTATCCnnnnn_n' Cuts at: 0 1072 3564 Size: 1072 2492 BclI T'GATC_A Cuts at: 0 371 2285 3398 3564 Size: 371 1914 1113 166 Fragments arranged by size: 1914 1113 371 166 BglI GCCn_nnn'nGGC Cuts at: 0 2218 3564 Size: 2218 1346 BglII A'GATC_T Cuts at: 0 803 3564 Size: 803 2761 Bme1580I G_kGCm'C Cuts at: 0 728 1005 3564 Size: 728 277 2559 Fragments arranged by size: 2559 728 277 BmgBI CAC'GTC Cuts at: 0 134 2277 3564 Size: 134 2143 1287 Fragments arranged by size: 2143 1287 134 BmrI ACTGGGnnnn_n' Cuts at: 0 1191 3564 Size: 1191 2373 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 2628 2968 3149 3322 3564 Size: 172 2456 340 181 173 242 Fragments arranged by size: 2456 340 242 181 173 172 Bpu10I CC'TnA_GC Cuts at: 0 2821 3196 3564 Size: 2821 375 368 Fragments arranged by size: 2821 375 368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 895 1148 1165 3470 3564 Size: 895 253 17 2305 94 Fragments arranged by size: 2305 895 253 94 17 BsaI GGTCTCn'nnnn_ Cuts at: 0 137 1025 3049 3564 Size: 137 888 2024 515 Fragments arranged by size: 2024 888 515 137 BsaAI yAC'GTr Cuts at: 0 682 2499 3564 Size: 682 1817 1065 Fragments arranged by size: 1817 1065 682 BsaBI GATnn'nnATC Cuts at: 0 1469 2163 3564 Size: 1469 694 1401 Fragments arranged by size: 1469 1401 694 BsaHI Gr'CG_yC Cuts at: 0 2465 3421 3461 3564 Size: 2465 956 40 103 Fragments arranged by size: 2465 956 103 40 BsaWI w'CCGG_w Cuts at: 0 2330 3564 Size: 2330 1234 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 509 539 2302 2332 3564 Size: 509 30 1763 30 1232 Fragments arranged by size: 1763 1232 509 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1397 1933 2349 2381 3319 3564 Size: 1397 536 416 32 938 245 Fragments arranged by size: 1397 938 536 416 245 32 BseYI C'CCAG_C Cuts at: 0 1611 2259 2831 3408 3564 Size: 1611 648 572 577 156 Fragments arranged by size: 1611 648 577 572 156 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 964 1347 2223 3331 3564 Size: 964 383 876 1108 233 Fragments arranged by size: 1108 964 876 383 233 BsiEI CG_ry'CG Cuts at: 0 3393 3564 Size: 3393 171 BsiHKAI G_wGCw'C Cuts at: 0 994 1971 3202 3564 Size: 994 977 1231 362 Fragments arranged by size: 1231 994 977 362 BsmI GAATG_Cn' Cuts at: 0 1487 3564 Size: 1487 2077 BsmBI CGTCTCn'nnnn_ Cuts at: 0 622 2453 3564 Size: 622 1831 1111 Fragments arranged by size: 1831 1111 622 Bsp1286I G_dGCh'C Cuts at: 0 728 994 1005 1971 3202 3564 Size: 728 266 11 966 1231 362 Fragments arranged by size: 1231 966 728 362 266 11 BspEI T'CCGG_A Cuts at: 0 2330 3564 Size: 2330 1234 BspHI T'CATG_A Cuts at: 0 1310 3564 Size: 1310 2254 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1251 3292 3564 Size: 1251 2041 272 Fragments arranged by size: 2041 1251 272 BsrBI CCG'CTC Cuts at: 0 65 3564 Size: 65 3499 BsrDI GCAATG_nn' Cuts at: 0 305 599 1331 3158 3295 3372 3564 Size: 305 294 732 1827 137 77 192 Fragments arranged by size: 1827 732 305 294 192 137 77 BsrGI T'GTAC_A Cuts at: 0 2744 3564 Size: 2744 820 BssSI C'ACGA_G Cuts at: 0 2358 3564 Size: 2358 1206 BstAPI GCAn_nnn'nTGC Cuts at: 0 439 3564 Size: 439 3125 BstBI TT'CG_AA Cuts at: 0 1740 2324 3564 Size: 1740 584 1240 Fragments arranged by size: 1740 1240 584 BstEII G'GTnAC_C Cuts at: 0 2622 3564 Size: 2622 942 BstYI r'GATC_y Cuts at: 0 59 803 947 3564 Size: 59 744 144 2617 Fragments arranged by size: 2617 744 144 59 BstZ17I GTA'TAC Cuts at: 0 1160 3564 Size: 1160 2404 Bsu36I CC'TnA_GG Cuts at: 0 587 3564 Size: 587 2977 BtgI C'CryG_G Cuts at: 0 46 71 1223 1859 3564 Size: 46 25 1152 636 1705 Fragments arranged by size: 1705 1152 636 46 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1675 2055 2544 3564 Size: 1675 380 489 1020 Fragments arranged by size: 1675 1020 489 380 BtsI GCAGTG_nn' Cuts at: 0 518 662 2235 2889 3564 Size: 518 144 1573 654 675 Fragments arranged by size: 1573 675 654 518 144 ClaI AT'CG_AT Cuts at: 0 2291 3564 Size: 2291 1273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 120 155 3564 Size: 120 35 3409 Fragments arranged by size: 3409 120 35 EaeI y'GGCC_r Cuts at: 0 224 1226 1511 2295 3564 Size: 224 1002 285 784 1269 Fragments arranged by size: 1269 1002 784 285 224 EarI CTCTTCn'nnn_ Cuts at: 0 273 307 1244 1575 1976 2929 3564 Size: 273 34 937 331 401 953 635 Fragments arranged by size: 953 937 635 401 331 273 34 EcoICRI GAG'CTC Cuts at: 0 992 1969 3200 3564 Size: 992 977 1231 364 Fragments arranged by size: 1231 992 977 364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 172 1367 1733 2326 2628 2968 3149 3322 Size: 172 1195 366 593 302 340 181 173 Cuts at: 3322 3564 Size: 242 Fragments arranged by size: 1195 593 366 340 302 242 181 173 172 EcoNI CCTnn'n_nnAGG Cuts at: 0 2191 3454 3564 Size: 2191 1263 110 Fragments arranged by size: 2191 1263 110 EcoO109I rG'GnC_Cy Cuts at: 0 76 724 1001 1002 3564 Size: 76 648 277 1 2562 Fragments arranged by size: 2562 648 277 76 1 EcoRI G'AATT_C Cuts at: 0 891 3564 Size: 891 2673 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1632 2062 2652 3564 Size: 1632 430 590 912 Fragments arranged by size: 1632 912 590 430 HaeII r_GCGC'y Cuts at: 0 907 2468 3564 Size: 907 1561 1096 Fragments arranged by size: 1561 1096 907 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 1048 1080 1392 1424 1538 1570 Size: 106 32 910 32 312 32 114 32 Cuts at: 1570 2958 2990 3564 Size: 1388 32 574 Fragments arranged by size: 1388 910 574 312 114 106 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 242 447 2111 2966 3564 Size: 242 205 1664 855 598 Fragments arranged by size: 1664 855 598 242 205 HindIII A'AGCT_T Cuts at: 0 2584 3564 Size: 2584 980 HpaI GTT'AAC Cuts at: 0 447 3564 Size: 447 3117 KasI G'GCGC_C Cuts at: 0 2464 3564 Size: 2464 1100 MfeI C'AATT_G Cuts at: 0 386 3564 Size: 386 3178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 120 364 1378 1958 2979 3564 Size: 120 244 1014 580 1021 585 Fragments arranged by size: 1021 1014 585 580 244 120 MscI TGG'CCA Cuts at: 0 1228 2297 3564 Size: 1228 1069 1267 Fragments arranged by size: 1267 1228 1069 MslI CAynn'nnrTG Cuts at: 0 361 740 1192 2358 2501 3473 3564 Size: 361 379 452 1166 143 972 91 Fragments arranged by size: 1166 972 452 379 361 143 91 MspA1I CmG'CkG Cuts at: 0 48 150 3564 Size: 48 102 3414 Fragments arranged by size: 3414 102 48 NarI GG'CG_CC Cuts at: 0 2465 3564 Size: 2465 1099 NcoI C'CATG_G Cuts at: 0 1223 3564 Size: 1223 2341 NdeI CA'TA_TG Cuts at: 0 2374 3018 3564 Size: 2374 644 546 Fragments arranged by size: 2374 644 546 NspI r_CATG'y Cuts at: 0 675 1304 2532 2820 3105 3564 Size: 675 629 1228 288 285 459 Fragments arranged by size: 1228 675 629 459 288 285 PflMI CCAn_nnn'nTGG Cuts at: 0 497 3065 3564 Size: 497 2568 499 Fragments arranged by size: 2568 499 497 PfoI T'CCnGG_A Cuts at: 0 1697 2342 3553 3564 Size: 1697 645 1211 11 Fragments arranged by size: 1697 1211 645 11 PmlI CAC'GTG Cuts at: 0 682 2499 3564 Size: 682 1817 1065 Fragments arranged by size: 1817 1065 682 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2668 2700 3564 Size: 2668 32 864 Fragments arranged by size: 2668 864 32 PpuMI rG'GwC_Cy Cuts at: 0 76 3564 Size: 76 3488 PshAI GACnn'nnGTC Cuts at: 0 1394 3564 Size: 1394 2170 PspOMI G'GGCC_C Cuts at: 0 724 1001 3564 Size: 724 277 2563 Fragments arranged by size: 2563 724 277 PstI C_TGCA'G Cuts at: 0 2383 2399 3455 3564 Size: 2383 16 1056 109 Fragments arranged by size: 2383 1056 109 16 PvuII CAG'CTG Cuts at: 0 150 3564 Size: 150 3414 SacI G_AGCT'C Cuts at: 0 994 1971 3202 3564 Size: 994 977 1231 362 Fragments arranged by size: 1231 994 977 362 SacII CC_GC'GG Cuts at: 0 49 3564 Size: 49 3515 SapI GCTCTTCn'nnn_ Cuts at: 0 273 1244 3564 Size: 273 971 2320 Fragments arranged by size: 2320 971 273 SfcI C'TryA_G Cuts at: 0 710 1413 2379 2395 3451 3564 Size: 710 703 966 16 1056 113 Fragments arranged by size: 1056 966 710 703 113 16 SfoI GGC'GCC Cuts at: 0 2466 3564 Size: 2466 1098 SmaI CCC'GGG Cuts at: 0 127 3564 Size: 127 3437 SmlI C'TyrA_G Cuts at: 0 31 910 1144 1163 3332 3485 3564 Size: 31 879 234 19 2169 153 79 Fragments arranged by size: 2169 879 234 153 79 31 19 SphI G_CATG'C Cuts at: 0 2532 2820 3105 3564 Size: 2532 288 285 459 Fragments arranged by size: 2532 459 288 285 SspI AAT'ATT Cuts at: 0 3006 3564 Size: 3006 558 StuI AGG'CCT Cuts at: 0 17 1567 3564 Size: 17 1550 1997 Fragments arranged by size: 1997 1550 17 StyI C'CwwG_G Cuts at: 0 789 983 1223 2247 2903 3564 Size: 789 194 240 1024 656 661 Fragments arranged by size: 1024 789 661 656 240 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2094 2491 2863 3564 Size: 2094 397 372 701 Fragments arranged by size: 2094 701 397 372 TatI w'GTAC_w Cuts at: 0 230 1007 2639 2744 3564 Size: 230 777 1632 105 820 Fragments arranged by size: 1632 820 777 230 105 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 509 541 1515 1547 1866 1898 3335 3367 Size: 509 32 974 32 319 32 1437 32 Cuts at: 3367 3564 Size: 197 Fragments arranged by size: 1437 974 509 319 197 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1131 2943 3564 Size: 1131 1812 621 Fragments arranged by size: 1812 1131 621 XhoI C'TCGA_G Cuts at: 0 3332 3564 Size: 3332 232 XmaI C'CCGG_G Cuts at: 0 125 3564 Size: 125 3439 XmnI GAAnn'nnTTC Cuts at: 0 1746 3564 Size: 1746 1818 Enzymes that do cut: AarI AccI AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NdeI NspI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SacII SapI SfcI SfoI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AfeI AgeI AloI ApaLI AscI AsiSI AvrII BaeI BbvCI BcgI BfrBI BlpI BmtI BplI BsiWI BsrFI BssHII BstXI DraI DraIII DrdI EagI EciI EcoRV FseI FspI FspAI KpnI MluI NaeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI ZraI