################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AgeI Alw44I AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BalI BamHI BbeI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsmAI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 242 GGTACC G^GTACC AccI 274 GTCTAC GT^[AC][GT]AC AhdI 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 412 AGCGCT AGC^GCT Asp718I 242 GGTACC G^GTACC AspEI 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 660 GTGCTC G[AT]GC[AT]^C AvaI 663 CTCGGG C^[CT]CG[GA]G AvaII 336 GGACC G^G[AT]CC BanI 242 GGTACC G^G[CT][GA]CC BbrPI 133 CACGTG CAC^GTG BclI 680 TGATCA T^GATCA BsiEI 357 CGACCG CG[GA][CT]^CG BsiHKAI 660 GTGCTC G[AT]GC[AT]^C BsiWI 293 CGTACG C^GTACG BsoBI 663 CTCGGG C^[CT]CG[GA]G Bsp1407I 137 TGTACA T^GTACA BsrBI 344 GAGCGG CCGCTC(-3/-3) BsrGI 137 TGTACA T^GTACA BssHII 96 GCGCGC G^CGCGC BstXI 470 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 194 CTGAG C^T[ACGT]AG DraII 435 GGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 535 CGGCCA [CT]^GGCC[GA] Eam1105I 121 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 412 AGCGCT AGC^GCT EcoO109I 435 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 20 CCCGC CCCGC(4/6) FbaI 680 TGATCA T^GATCA HaeII 412 AGCGCT [GA]GCGC^[CT] HgaI 210 GCGTC GACGC(5/10) HinfI 466 GACTC G^A[ACGT]TC KpnI 242 GGTACC GGTAC^C MboII 513 GAAGA GAAGA(8/7) MslI 153 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 500 GCATGC [GA]CATG^[CT] PleI 466 GACTC GAGTC(4/5) PmaCI 133 CACGTG CAC^GTG PmlI 133 CACGTG CAC^GTG PstI 427 CTGCAG CTGCA^G SfaNI 625 GATGC GCATC(5/9) SfcI 427 CTGCAG C^T[GA][CT]AG SphI 500 GCATGC GCATG^C SspBI 137 TGTACA T^GTACA TspRI 321 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 470 CCAAGTGTCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 366 GATCC GGATC(4/5) 692 GGATC BanII 444 GGGCTC G[GA]GC[CT]^C 637 GGGCTC BpmI 247 CTGGAG CTGGAG(16/14) 604 CTGGAG BsaAI 133 CACGTG [CT]AC^GT[GA] 291 CACGTA BsrI 689 ACTGG ACTGG(1/-1) 320 CCAGT DsaI 381 CCACGG C^C[GA][CT]GG 590 CCACGG HphI 303 GGTGA GGTGA(8/7) 560 TCACC PvuII 397 CAGCTG CAG^CTG 430 CAGCTG TaqI 35 TCGA T^CGA 163 TCGA TthHB8I 35 TCGA T^CGA 163 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwNI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI