Restriction map for
pFN21AE6638
(Circular) (Six-base) MAPSORT of: pFN21AE6638.seq Check: 4919 from: 1 to: 5416
pFN21AE6638 5416 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3226 278
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2280 2759 1937
Size: 343 479 4594
Fragments arranged by size:
4594 479 343
Acc65I G'GTAC_C
Cuts at: 2247 2737 2247
Size: 490 4926
AclI AA'CG_TT
Cuts at: 1563 3543 3916 1563
Size: 1980 373 3063
Fragments arranged by size:
3063 1980 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2246 2537 3479 4863 5131 1632
Size: 614 291 942 1384 268 1917
Fragments arranged by size:
1917 1384 942 614 291 268
AfeI AGC'GCT
Cuts at: 2419 2419
Size: 5416
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5219
AflIII A'CryG_T
Cuts at: 1203 2139 2193 2259 4315 5292 1203
Size: 936 54 66 2056 977 1327
Fragments arranged by size:
2056 1327 977 936 66 54
AgeI A'CCGG_T
Cuts at: 4247 4247
Size: 5416
AhdI GACnn_n'nnGTC
Cuts at: 2131 4145 2131
Size: 2014 3402
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2114 1184
Size: 930 4486
AlwNI CAG_nnn'CTG
Cuts at: 2224 2554 2694 3159 4731 2224
Size: 330 140 465 1572 2909
Fragments arranged by size:
2909 1572 465 330 140
ApaLI G'TGCA_C
Cuts at: 3473 4629 3473
Size: 1156 4260
ApoI r'AATT_y
Cuts at: 1493 2725 2951 3103 5212 1493
Size: 1232 226 152 2109 1697
Fragments arranged by size:
2109 1697 1232 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3970 160
Size: 872 2938 1606
Fragments arranged by size:
2938 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
AvaI C'yCGr_G
Cuts at: 1870 1943 2668 2741 1870
Size: 73 725 73 4545
Fragments arranged by size:
4545 725 73 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2648 2681 2648
Size: 33 5383
BamHI G'GATC_C
Cuts at: 2746 3113 2746
Size: 367 5049
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2247 2737 4192 618
Size: 325 217 627 460 490 1455 1842
Fragments arranged by size:
1842 1455 627 490 460 325 217
BanII G_rGCy'C
Cuts at: 1391 2453 2646 2735 1391
Size: 1062 193 89 4072
Fragments arranged by size:
4072 1062 193 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4445
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3607 3641 1903
Size: 34 1670 34 3678
Fragments arranged by size:
3678 1670 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3308 4518 3308
Size: 1210 4206
BclI T'GATC_A
Cuts at: 1548 2685 1548
Size: 1137 4279
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4027 136
Size: 107 122 71 631 605 2355 1525
Fragments arranged by size:
2355 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5411 5411
Size: 5416
BlpI GC'TnA_GC
Cuts at: 2833 2833
Size: 5416
Bme1580I G_kGCm'C
Cuts at: 1790 3477 4633 1790
Size: 1687 1156 2573
Fragments arranged by size:
2573 1687 1156
BmgBI CAC'GTC
Cuts at: 1318 2194 1318
Size: 876 4540
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3147 4105 442
Size: 416 53 83 399 24 1730 958 1753
Fragments arranged by size:
1753 1730 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2273 2630 4076 1239
Size: 1034 357 1446 2579
Fragments arranged by size:
2579 1446 1034 357
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3541 4406 4704 3541
Size: 865 298 4253
Fragments arranged by size:
4253 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4079 882
Size: 617 2580 2219
Fragments arranged by size:
2580 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2140 2298 3207 493
Size: 711 936 158 909 2702
Fragments arranged by size:
2702 936 909 711 158
BsaBI GATnn'nnATC
Cuts at: 5410 5410
Size: 5416
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3223 3605 4240 275
Size: 53 83 186 936 1690 382 635 1451
Fragments arranged by size:
1690 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3849 4247 4521 4668 5079 3849
Size: 398 274 147 411 4186
Fragments arranged by size:
4186 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2246 2276 1178
Size: 30 165 30 843 30 4318
Fragments arranged by size:
4318 843 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5416
BseYI C'CCAG_C
Cuts at: 2400 2437 4619 5115 2400
Size: 37 2182 496 2701
Fragments arranged by size:
2701 2182 496 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4843
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2365 2866 3627 4655 1540
Size: 275 186 364 501 761 1028 2301
Fragments arranged by size:
2301 1028 761 501 364 275 186
BsiHKAI G_wGCw'C
Cuts at: 2669 3477 3562 4633 2669
Size: 808 85 1071 3452
Fragments arranged by size:
3452 1071 808 85
BsiWI C'GTAC_G
Cuts at: 2298 2298
Size: 5416
BsmI GAATG_Cn'
Cuts at: 2927 3020 2927
Size: 93 5323
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2453 2646 2669 2735 3477 3562 4633
Size: 399 663 193 23 66 742 85 1071
Cuts at: 4633 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 663 399 193 85 66
23
BspEI T'CCGG_A
Cuts at: 5079 5079
Size: 5416
BspHI T'CATG_A
Cuts at: 3305 5035 3305
Size: 1730 3686
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2771 844
Size: 1927 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2351 3303 1394
Size: 429 528 952 3507
Fragments arranged by size:
3507 952 528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3911 4085 65
Size: 1183 2663 174 1396
Fragments arranged by size:
2663 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4060 4247 1700
Size: 110 2250 187 2869
Fragments arranged by size:
2869 2250 187 110
BsrGI T'GTAC_A
Cuts at: 96 2142 96
Size: 2046 3370
BssHII G'CGCG_C
Cuts at: 1925 2101 1925
Size: 176 5240
BssSI C'ACGA_G
Cuts at: 3476 4488 3476
Size: 1012 4404
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5416
BstBI TT'CG_AA
Cuts at: 4220 4220
Size: 5416
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5416
BstXI CCAn_nnnn'nTGG
Cuts at: 2482 2482
Size: 5416
BstYI r'GATC_y
Cuts at: 1972 2746 3113 3498 3515 4967 5053 5065 5411
Size: 774 367 385 17 1452 86 12 346
Cuts at: 5411 1972
Size: 1977
Fragments arranged by size:
1977 1452 774 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2386 2595 513
Size: 552 939 382 209 3334
Fragments arranged by size:
3334 939 552 382 209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4677
Fragments arranged by size:
4677 683 56
BtsI GCAGTG_nn'
Cuts at: 2932 3726 3746 2932
Size: 794 20 4602
Fragments arranged by size:
4602 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5416
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5381
DraI TTT'AAA
Cuts at: 2720 2720
Size: 5416
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4423 809
Size: 507 3107 1802
Fragments arranged by size:
3107 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2540 2863 3752
Size: 54 1425 50 275 18 710 323 889
Cuts at: 3752 5381 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 710 323 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2863 1537
Size: 275 1051 4090
Fragments arranged by size:
4090 1051 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3346 1353
Size: 1993 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4003 4377 4523 387
Size: 1064 138 2414 374 146 1280
Fragments arranged by size:
2414 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2273 2537 2630 3479 4076 4863
Size: 393 614 27 264 93 849 597 787
Cuts at: 4863 5131 1239
Size: 268 1524
Fragments arranged by size:
1524 849 787 614 597 393 268 264
93 27
EcoO109I rG'GnC_Cy
Cuts at: 2441 2441
Size: 5416
EcoRI G'AATT_C
Cuts at: 2725 3103 2725
Size: 378 5038
FspI TGC'GCA
Cuts at: 3922 3922
Size: 5416
HaeII r_GCGC'y
Cuts at: 1678 2421 4563 1678
Size: 743 2142 2531
Fragments arranged by size:
2531 2142 743
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 4057 4089 4131
Size: 32 135 32 545 32 1908 32 42
Cuts at: 4131 4163 1373
Size: 32 2626
Fragments arranged by size:
2626 1908 545 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2760 3006 669
Size: 988 281 822 246 3079
Fragments arranged by size:
3079 988 822 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5416
HpaI GTT'AAC
Cuts at: 3006 3006
Size: 5416
KpnI G_GTAC'C
Cuts at: 2251 2741 2251
Size: 490 4926
MfeI C'AATT_G
Cuts at: 3015 3015
Size: 5416
MluI A'CGCG_T
Cuts at: 5292 5292
Size: 5416
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4530 4714 1305
Size: 3225 184 2007
Fragments arranged by size:
3225 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3594
Fragments arranged by size:
3594 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2162 3374 3733 3892 518
Size: 648 843 153 1212 359 159 2042
Fragments arranged by size:
2042 1212 843 648 359 159 153
MspA1I CmG'CkG
Cuts at: 1606 2404 2437 2551 2604 2832 3509 4657 4902
Size: 798 33 114 53 228 677 1148 245
Cuts at: 4902 1606
Size: 2120
Fragments arranged by size:
2120 1148 798 677 245 228 114 53
33
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3925
Fragments arranged by size:
3925 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5416
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5416
NotI GC'GGCC_GC
Cuts at: 2863 2863
Size: 5416
NspI r_CATG'y
Cuts at: 1132 1229 2509 2774 4319 1132
Size: 97 1280 265 1545 2229
Fragments arranged by size:
2229 1545 1280 265 97
PasI CC'CwG_GG
Cuts at: 1348 2174 1348
Size: 826 4590
PciI A'CATG_T
Cuts at: 4315 4315
Size: 5416
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5416
PmeI GTTT'AAAC
Cuts at: 2720 2720
Size: 5416
PmlI CAC'GTG
Cuts at: 2140 2140
Size: 5416
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3485 3517 4998 5030 3485
Size: 32 1481 32 3871
Fragments arranged by size:
3871 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5416
PsiI TTA'TAA
Cuts at: 2986 2986
Size: 5416
PstI C_TGCA'G
Cuts at: 830 2436 2768 830
Size: 1606 332 3478
Fragments arranged by size:
3478 1606 332
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5416
PvuII CAG'CTG
Cuts at: 2404 2437 2404
Size: 33 5383
SalI G'TCGA_C
Cuts at: 1936 2758 1936
Size: 822 4594
SbfI CC_TGCA'GG
Cuts at: 2768 2768
Size: 5416
ScaI AGT'ACT
Cuts at: 3664 5376 3664
Size: 1712 3704
SfcI C'TryA_G
Cuts at: 826 1045 2432 2764 3899 4580 4771 826
Size: 219 1387 332 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 681 332 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5416
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5416
SmaI CCC'GGG
Cuts at: 1872 2743 1872
Size: 871 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3520 4421 4683 820
Size: 197 926 1577 901 262 1553
Fragments arranged by size:
1577 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3207 493
Size: 2714 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5416
SphI G_CATG'C
Cuts at: 1132 2509 2774 1132
Size: 1377 265 3774
Fragments arranged by size:
3774 1377 265
SspI AAT'ATT
Cuts at: 5 52 3340 5
Size: 47 3288 2081
Fragments arranged by size:
3288 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5416
StyI C'CwwG_G
Cuts at: 513 1065 2004 2855 513
Size: 552 939 851 3074
Fragments arranged by size:
3074 939 851 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2582 3443 3460 3613 3798 4243 325
Size: 1255 1002 861 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1002 861 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2142 3662 5374
Size: 274 80 33 51 1443 165 1520 1712
Cuts at: 5374 96
Size: 138
Fragments arranged by size:
1712 1520 1443 274 165 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2378 2410 5376 5408 2378
Size: 32 2966 32 2386
Fragments arranged by size:
2966 2386 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5416
XbaI T'CTAG_A
Cuts at: 2752 2752
Size: 5416
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2482 2482
Size: 5416
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5416
XmaI C'CCGG_G
Cuts at: 1870 2741 1870
Size: 871 4545
XmnI GAAnn'nnTTC
Cuts at: 3545 3545
Size: 5416
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3224 276
Size: 53 83 186 936 1690 2468
Fragments arranged by size:
2468 1690 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI