Restriction map for
pFN21AE6638
(Linear) (Six-base) MAPSORT of: KIEE6638.seq Check: 7248 from: 1 to: 711
KIEE6638.orf
With 182 enzymes: SgfI *
March 16, 2015 11:34 ..
AccI GT'mk_AC
Cuts at: 0 275 711
Size: 275 436
Acc65I G'GTAC_C
Cuts at: 0 242 711
Size: 242 469
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 532 711
Size: 241 291 179
Fragments arranged by size:
291 241 179
AfeI AGC'GCT
Cuts at: 0 414 711
Size: 414 297
AflIII A'CryG_T
Cuts at: 0 134 188 254 711
Size: 134 54 66 457
Fragments arranged by size:
457 134 66 54
AhdI GACnn_n'nnGTC
Cuts at: 0 126 711
Size: 126 585
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 109 711
Size: 109 602
AlwNI CAG_nnn'CTG
Cuts at: 0 219 549 689 711
Size: 219 330 140 22
Fragments arranged by size:
330 219 140 22
AvaI C'yCGr_G
Cuts at: 0 663 711
Size: 663 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 643 676 711
Size: 643 33 35
Fragments arranged by size:
643 35 33
BanI G'GyrC_C
Cuts at: 0 242 711
Size: 242 469
BanII G_rGCy'C
Cuts at: 0 448 641 711
Size: 448 193 70
Fragments arranged by size:
448 193 70
BclI T'GATC_A
Cuts at: 0 680 711
Size: 680 31
BmgBI CAC'GTC
Cuts at: 0 189 711
Size: 189 522
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 625 711
Size: 268 357 86
Fragments arranged by size:
357 268 86
BsaAI yAC'GTr
Cuts at: 0 135 293 711
Size: 135 158 418
Fragments arranged by size:
418 158 135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 711
Size: 241 30 440
Fragments arranged by size:
440 241 30
BseYI C'CCAG_C
Cuts at: 0 395 432 711
Size: 395 37 279
Fragments arranged by size:
395 279 37
BsiEI CG_ry'CG
Cuts at: 0 360 711
Size: 360 351
BsiHKAI G_wGCw'C
Cuts at: 0 664 711
Size: 664 47
BsiWI C'GTAC_G
Cuts at: 0 293 711
Size: 293 418
Bsp1286I G_dGCh'C
Cuts at: 0 448 641 664 711
Size: 448 193 23 47
Fragments arranged by size:
448 193 47 23
BsrBI CCG'CTC
Cuts at: 0 346 711
Size: 346 365
BsrGI T'GTAC_A
Cuts at: 0 137 711
Size: 137 574
BssHII G'CGCG_C
Cuts at: 0 96 711
Size: 96 615
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 711
Size: 477 234
BtgI C'CryG_G
Cuts at: 0 381 590 711
Size: 381 209 121
Fragments arranged by size:
381 209 121
EaeI y'GGCC_r
Cuts at: 0 535 711
Size: 535 176
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 268 532 625 711
Size: 241 27 264 93 86
Fragments arranged by size:
264 241 93 86 27
EcoO109I rG'GnC_Cy
Cuts at: 0 436 711
Size: 436 275
HaeII r_GCGC'y
Cuts at: 0 416 711
Size: 416 295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 711
Size: 112 32 567
Fragments arranged by size:
567 112 32
KpnI G_GTAC'C
Cuts at: 0 246 711
Size: 246 465
MslI CAynn'nnrTG
Cuts at: 0 157 711
Size: 157 554
MspA1I CmG'CkG
Cuts at: 0 399 432 546 599 711
Size: 399 33 114 53 112
Fragments arranged by size:
399 114 112 53 33
NspI r_CATG'y
Cuts at: 0 504 711
Size: 504 207
PasI CC'CwG_GG
Cuts at: 0 169 711
Size: 169 542
PmlI CAC'GTG
Cuts at: 0 135 711
Size: 135 576
PstI C_TGCA'G
Cuts at: 0 431 711
Size: 431 280
PvuII CAG'CTG
Cuts at: 0 399 432 711
Size: 399 33 279
Fragments arranged by size:
399 279 33
SfcI C'TryA_G
Cuts at: 0 427 711
Size: 427 284
SphI G_CATG'C
Cuts at: 0 504 711
Size: 504 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 711
Size: 577 134
TatI w'GTAC_w
Cuts at: 0 137 711
Size: 137 574
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 711
Size: 373 32 306
Fragments arranged by size:
373 306 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 711
Size: 477 234
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AlfI AlwNI
AvaI BaeI BanI BanII BclI BmgBI BpmI BsaAI
BsaXI BseYI BsiEI BsiHKAI BsiWI Bsp1286I BsrBI BsrGI
BssHII BstXI BtgI EaeI Eco57MI EcoO109I HaeII Hin4I
KpnI MslI MspA1I NspI PasI PmlI PstI PvuII
SfcI SphI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AleI AloI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BseRI BsgI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI