(Circular) (Six-base) MAPSORT of: pFN21AE9633.seq Check: 1136 from: 1 to: 8713 pFN21AE9633 8713 bp With 182 enzymes: SgfI * January 16, 2014 19:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3403 3403 Size: 8713 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2388 6523 278 Size: 53 83 186 936 852 4135 2468 Fragments arranged by size: 4135 2468 936 852 186 83 53 AccI GT'mk_AC Cuts at: 1937 2583 2952 2964 3468 6056 1937 Size: 646 369 12 504 2588 4594 Fragments arranged by size: 4594 2588 646 504 369 12 Acc65I G'GTAC_C Cuts at: 2757 6034 2757 Size: 3277 5436 AclI AA'CG_TT Cuts at: 1563 6840 7213 1563 Size: 5277 373 3063 Fragments arranged by size: 5277 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 2645 2924 3566 3680 4978 5209 6776 Size: 559 454 279 642 114 1298 231 1567 Cuts at: 6776 8160 8428 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1567 1384 1298 642 559 454 279 268 231 114 AfeI AGC'GCT Cuts at: 3134 3134 Size: 8713 AflII C'TTAA_G Cuts at: 820 1017 4103 820 Size: 197 3086 5430 Fragments arranged by size: 5430 3086 197 AflIII A'CryG_T Cuts at: 1203 2607 3114 7612 8589 1203 Size: 1404 507 4498 977 1327 Fragments arranged by size: 4498 1404 1327 977 507 AgeI A'CCGG_T Cuts at: 7544 7544 Size: 8713 AhdI GACnn_n'nnGTC Cuts at: 5381 7442 5381 Size: 2061 6652 AleI CACnn'nnGTG Cuts at: 1166 3983 1166 Size: 2817 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4308 1184 Size: 3124 5589 AlwNI CAG_nnn'CTG Cuts at: 2623 4438 4533 5277 5745 5860 6456 8028 2623 Size: 1815 95 744 468 115 596 1572 3308 Fragments arranged by size: 3308 1815 1572 744 596 468 115 95 ApaI G_GGCC'C Cuts at: 3776 3920 4784 5046 3776 Size: 144 864 262 7443 Fragments arranged by size: 7443 864 262 144 ApaLI G'TGCA_C Cuts at: 2255 4397 6770 7926 2255 Size: 2142 2373 1156 3042 Fragments arranged by size: 3042 2373 2142 1156 ApoI r'AATT_y Cuts at: 1493 2111 3062 6022 6248 6400 8509 1493 Size: 618 951 2960 226 152 2109 1697 Fragments arranged by size: 2960 2109 1697 951 618 226 152 AseI AT'TA_AT Cuts at: 160 1032 7267 160 Size: 872 6235 1606 Fragments arranged by size: 6235 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 AvaI C'yCGr_G Cuts at: 1870 1943 2492 3184 3374 3608 3920 4682 4697 Size: 73 549 692 190 234 312 762 15 Cuts at: 4697 4966 5019 5802 6038 1870 Size: 269 53 783 236 4545 Fragments arranged by size: 4545 783 762 692 549 312 269 236 234 190 73 53 15 BamHI G'GATC_C Cuts at: 2940 6043 6410 2940 Size: 3103 367 5243 Fragments arranged by size: 5243 3103 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2757 3307 3828 4321 4357 Size: 325 217 627 970 550 521 493 36 Cuts at: 4357 4702 5012 5031 5792 6034 7489 618 Size: 345 310 19 761 242 1455 1842 Fragments arranged by size: 1842 1455 970 761 627 550 521 493 345 325 310 242 217 36 19 BanII G_rGCy'C Cuts at: 1391 3776 3823 3920 4784 4866 4956 5007 5046 Size: 2385 47 97 864 82 90 51 39 Cuts at: 5046 5457 6032 1391 Size: 411 575 4072 Fragments arranged by size: 4072 2385 864 575 411 97 90 82 51 47 39 BbeI G_GCGC'C Cuts at: 3832 4325 4706 3832 Size: 493 381 7839 Fragments arranged by size: 7839 493 381 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3734 4023 5215 928 Size: 971 1835 289 1192 4426 Fragments arranged by size: 4426 1835 1192 971 289 BbvCI CC'TCA_GC Cuts at: 4526 5234 5396 4526 Size: 708 162 7843 Fragments arranged by size: 7843 708 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4339 4373 6904 6938 1903 Size: 34 2402 34 2531 34 3678 Fragments arranged by size: 3678 2531 2402 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5120 6605 7815 5120 Size: 1485 1210 6018 Fragments arranged by size: 6018 1485 1210 BclI T'GATC_A Cuts at: 1548 3250 5851 1548 Size: 1702 2601 4410 Fragments arranged by size: 4410 2601 1702 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4663 4960 5667 Size: 107 122 71 631 605 2991 297 707 Cuts at: 5667 7324 136 Size: 1657 1525 Fragments arranged by size: 2991 1657 1525 707 631 605 297 122 107 71 BglII A'GATC_T Cuts at: 8708 8708 Size: 8713 BlpI GC'TnA_GC Cuts at: 4841 4853 5426 6130 4841 Size: 12 573 704 7424 Fragments arranged by size: 7424 704 573 12 Bme1580I G_kGCm'C Cuts at: 1790 2259 2450 3012 3310 3776 3920 4401 4784 Size: 469 191 562 298 466 144 481 383 Cuts at: 4784 5046 6774 7930 1790 Size: 262 1728 1156 2573 Fragments arranged by size: 2573 1728 1156 562 481 469 466 383 298 262 191 144 BmgBI CAC'GTC Cuts at: 1318 2179 2422 4396 5194 5755 1318 Size: 861 243 1974 798 561 4276 Fragments arranged by size: 4276 1974 861 798 561 243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 2461 2605 Size: 416 53 83 399 24 937 107 144 Cuts at: 2605 3708 6444 7402 442 Size: 1103 2736 958 1753 Fragments arranged by size: 2736 1753 1103 958 937 416 399 144 107 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8713 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2322 2324 4527 2322 Size: 2 2203 6508 Fragments arranged by size: 6508 2203 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2573 3125 3248 3659 4723 5497 7373 Size: 995 339 552 123 411 1064 774 1876 Cuts at: 7373 1239 Size: 2579 Fragments arranged by size: 2579 1876 1064 995 774 552 411 339 123 Bpu10I CC'TnA_GC Cuts at: 4526 5234 5396 4526 Size: 708 162 7843 Fragments arranged by size: 7843 708 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4232 6838 7703 8001 4232 Size: 2606 865 298 4944 Fragments arranged by size: 4944 2606 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2865 3514 5500 7376 882 Size: 617 1366 649 1986 1876 2219 Fragments arranged by size: 2219 1986 1876 1366 649 617 BsaAI yAC'GTr Cuts at: 493 1204 2293 3115 6504 493 Size: 711 1089 822 3389 2702 Fragments arranged by size: 3389 2702 1089 822 711 BsaBI GATnn'nnATC Cuts at: 8707 8707 Size: 8713 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2385 2489 3829 4322 Size: 53 83 186 936 852 104 1340 493 Cuts at: 4322 4703 6520 6902 7537 275 Size: 381 1817 382 635 1451 Fragments arranged by size: 1817 1451 1340 936 852 635 493 382 381 186 104 83 53 BsaWI w'CCGG_w Cuts at: 2933 3804 5529 5640 7146 7544 7818 7965 8376 Size: 871 1725 111 1506 398 274 147 411 Cuts at: 8376 2933 Size: 3270 Fragments arranged by size: 3270 1725 1506 871 411 398 274 147 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2862 2892 3478 3508 4297 Size: 30 165 30 1459 30 586 30 789 Cuts at: 4297 4327 4531 4561 4721 4751 1178 Size: 30 204 30 160 30 5140 Fragments arranged by size: 5140 1459 789 586 204 165 160 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2231 2843 3251 3254 3281 3284 3287 Size: 1012 33 612 408 3 27 3 3 Cuts at: 3287 3299 4046 4318 4331 4477 4802 4964 1186 Size: 12 747 272 13 146 325 162 4935 Fragments arranged by size: 4935 1012 747 612 408 325 272 162 146 33 27 13 12 3 3 3 BseYI C'CCAG_C Cuts at: 2499 2616 3129 3194 3769 4152 4760 5038 5045 Size: 117 513 65 575 383 608 278 7 Cuts at: 5045 7916 8412 2499 Size: 2871 496 2800 Fragments arranged by size: 2871 2800 608 575 513 496 383 278 117 65 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2792 2978 3074 1163 Size: 573 1056 186 96 6802 Fragments arranged by size: 6802 1056 573 186 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6163 6924 7952 1540 Size: 275 186 4162 761 1028 2301 Fragments arranged by size: 4162 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2259 2559 3669 3823 4070 4347 4401 4866 6774 Size: 300 1110 154 247 277 54 465 1908 Cuts at: 6774 6859 7930 2259 Size: 85 1071 3042 Fragments arranged by size: 3042 1908 1110 1071 465 300 277 247 154 85 54 BsmI GAATG_Cn' Cuts at: 6224 6317 6224 Size: 93 8620 BsmBI CGTCTCn'nnnn_ Cuts at: 2376 2480 5389 2376 Size: 104 2909 5700 Fragments arranged by size: 5700 2909 104 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2259 2450 2559 3012 3310 3669 3776 Size: 399 469 191 109 453 298 359 107 Cuts at: 3776 3823 3920 4070 4347 4401 4784 4866 4956 Size: 47 97 150 277 54 383 82 90 Cuts at: 4956 5007 5046 5457 6032 6774 6859 7930 1391 Size: 51 39 411 575 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 575 469 453 411 399 383 359 298 277 191 150 109 107 97 90 85 82 54 51 47 39 BspEI T'CCGG_A Cuts at: 5529 5640 8376 5529 Size: 111 2736 5866 Fragments arranged by size: 5866 2736 111 BspHI T'CATG_A Cuts at: 6602 8332 6602 Size: 1730 6983 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3403 4239 4300 4323 5784 6068 844 Size: 2559 836 61 23 1461 284 3489 Fragments arranged by size: 3489 2559 1461 836 284 61 23 BsrBI CCG'CTC Cuts at: 1394 1823 4737 6600 1394 Size: 429 2914 1863 3507 Fragments arranged by size: 3507 2914 1863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2655 4084 7208 7382 65 Size: 1183 1407 1429 3124 174 1396 Fragments arranged by size: 3124 1429 1407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2406 3488 4178 5442 7357 7544 1700 Size: 110 596 1082 690 1264 1915 187 2869 Fragments arranged by size: 2869 1915 1264 1082 690 596 187 110 BsrGI T'GTAC_A Cuts at: 96 2514 96 Size: 2418 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8713 BssSI C'ACGA_G Cuts at: 4871 6773 7785 4871 Size: 1902 1012 5799 Fragments arranged by size: 5799 1902 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8713 BstBI TT'CG_AA Cuts at: 7517 7517 Size: 8713 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8713 BstXI CCAn_nnnn'nTGG Cuts at: 3460 4152 3460 Size: 692 8021 BstYI r'GATC_y Cuts at: 1972 2027 2940 3024 4541 4875 5581 6043 6410 Size: 55 913 84 1517 334 706 462 367 Cuts at: 6410 6795 6812 8264 8350 8362 8708 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1517 1452 913 706 462 385 367 346 334 86 84 55 17 12 Bsu36I CC'TnA_GG Cuts at: 3261 4403 5903 3261 Size: 1142 1500 6071 Fragments arranged by size: 6071 1500 1142 BtgI C'CryG_G Cuts at: 513 1065 2004 2200 2262 3501 3783 4752 513 Size: 552 939 196 62 1239 282 969 4474 Fragments arranged by size: 4474 1239 969 939 552 282 196 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3853 3943 4446 487 Size: 683 56 2627 90 503 4754 Fragments arranged by size: 4754 2627 683 503 90 56 BtsI GCAGTG_nn' Cuts at: 3959 5329 5781 6229 7023 7043 3959 Size: 1370 452 448 794 20 5629 Fragments arranged by size: 5629 1370 794 452 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8713 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5860 5895 529 Size: 35 5296 35 3347 Fragments arranged by size: 5296 3347 35 35 DraI TTT'AAA Cuts at: 6017 6017 Size: 8713 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7720 809 Size: 507 6404 1802 Fragments arranged by size: 6404 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 3462 3690 Size: 54 1425 50 275 18 291 1341 228 Cuts at: 3690 3719 3795 4647 4899 5135 5217 6160 7049 Size: 29 76 852 252 236 82 943 889 Cuts at: 7049 8678 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1341 943 889 852 291 275 252 236 228 82 76 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 6160 1537 Size: 275 4348 4090 Fragments arranged by size: 4348 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3217 4023 6643 1353 Size: 1864 806 2620 3423 Fragments arranged by size: 3423 2620 1864 806 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2997 5696 7300 7674 7820 387 Size: 1064 138 1408 2699 1604 374 146 1280 Fragments arranged by size: 2699 1604 1408 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3821 4864 3821 Size: 1043 7670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2234 2573 2645 2924 3125 3248 Size: 393 559 43 339 72 279 201 123 Cuts at: 3248 3566 3659 3680 4723 4978 5209 5497 6776 Size: 318 93 21 1043 255 231 288 1279 Cuts at: 6776 7373 8160 8428 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1279 1043 787 597 559 393 339 318 288 279 268 255 231 201 123 93 72 43 21 EcoNI CCTnn'n_nnAGG Cuts at: 3146 4247 3146 Size: 1101 7612 EcoO109I rG'GnC_Cy Cuts at: 3370 3550 3622 3703 3916 3917 4506 4757 4780 Size: 180 72 81 213 1 589 251 23 Cuts at: 4780 5042 5274 3370 Size: 262 232 6809 Fragments arranged by size: 6809 589 262 251 232 213 180 81 72 23 1 EcoRI G'AATT_C Cuts at: 6022 6400 6022 Size: 378 8335 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4610 4610 Size: 8713 FspI TGC'GCA Cuts at: 4379 5054 7219 4379 Size: 675 2165 5873 Fragments arranged by size: 5873 2165 675 HaeII r_GCGC'y Cuts at: 1678 2134 2822 3136 3832 4325 4646 4669 4706 Size: 456 688 314 696 493 321 23 37 Cuts at: 4706 5125 7860 1678 Size: 419 2735 2531 Fragments arranged by size: 2735 2531 696 688 493 456 419 321 314 37 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2156 2188 3194 3226 4331 Size: 32 135 32 584 32 1006 32 1105 Cuts at: 4331 4363 4531 4533 4563 4565 5367 5399 5573 Size: 32 168 2 30 2 802 32 174 Cuts at: 5573 5605 5611 5643 7354 7386 7428 7460 1373 Size: 32 6 32 1711 32 42 32 2626 Fragments arranged by size: 2626 1711 1105 1006 802 584 174 168 135 42 32 32 32 32 32 32 32 32 32 32 30 6 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4132 6057 6303 669 Size: 988 281 2194 1925 246 3079 Fragments arranged by size: 3079 2194 1925 988 281 246 HindIII A'AGCT_T Cuts at: 748 2222 4592 748 Size: 1474 2370 4869 Fragments arranged by size: 4869 2370 1474 HpaI GTT'AAC Cuts at: 6303 6303 Size: 8713 KasI G'GCGC_C Cuts at: 3828 4321 4702 3828 Size: 493 381 7839 Fragments arranged by size: 7839 493 381 KpnI G_GTAC'C Cuts at: 2761 6038 2761 Size: 3277 5436 MfeI C'AATT_G Cuts at: 6312 6312 Size: 8713 MluI A'CGCG_T Cuts at: 8589 8589 Size: 8713 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2154 2597 2825 2918 3414 4251 4317 4908 Size: 849 443 228 93 496 837 66 591 Cuts at: 4908 7827 8011 1305 Size: 2919 184 2007 Fragments arranged by size: 2919 2007 849 837 591 496 443 228 184 93 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 3692 3721 4649 4901 10 Size: 54 1425 343 1860 29 928 252 3822 Fragments arranged by size: 3822 1860 1425 928 343 252 54 29 MslI CAynn'nnrTG Cuts at: 518 1166 2453 3672 3983 4058 6671 7030 7189 Size: 648 1287 1219 311 75 2613 359 159 Cuts at: 7189 518 Size: 2042 Fragments arranged by size: 2613 2042 1287 1219 648 359 311 159 75 MspA1I CmG'CkG Cuts at: 1606 2202 2236 2503 3019 3785 4754 5038 5286 Size: 596 34 267 516 766 969 284 248 Cuts at: 5286 5400 5425 5524 6129 6806 7954 8199 1606 Size: 114 25 99 605 677 1148 245 2120 Fragments arranged by size: 2120 1148 969 766 677 605 596 516 284 267 248 245 114 99 34 25 NaeI GCC'GGC Cuts at: 2408 4180 2408 Size: 1772 6941 NarI GG'CG_CC Cuts at: 3829 4322 4703 3829 Size: 493 381 7839 Fragments arranged by size: 7839 493 381 NcoI C'CATG_G Cuts at: 513 1065 2004 2262 513 Size: 552 939 258 6964 Fragments arranged by size: 6964 939 552 258 NdeI CA'TA_TG Cuts at: 387 387 Size: 8713 NgoMIV G'CCGG_C Cuts at: 2406 4178 2406 Size: 1772 6941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8713 NotI GC'GGCC_GC Cuts at: 6160 6160 Size: 8713 NspI r_CATG'y Cuts at: 1132 1229 2063 2464 2611 5263 5716 6071 7616 Size: 97 834 401 147 2652 453 355 1545 Cuts at: 7616 1132 Size: 2229 Fragments arranged by size: 2652 2229 1545 834 453 401 355 147 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8713 PciI A'CATG_T Cuts at: 2607 7612 2607 Size: 5005 3708 PflMI CCAn_nnn'nTGG Cuts at: 1108 5551 1108 Size: 4443 4270 PfoI T'CCnGG_A Cuts at: 3102 3585 3636 4616 5457 3102 Size: 483 51 980 841 6358 Fragments arranged by size: 6358 980 841 483 51 PmeI GTTT'AAAC Cuts at: 6017 6017 Size: 8713 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6782 6814 8295 8327 6782 Size: 32 1481 32 7168 Fragments arranged by size: 7168 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3622 3703 4757 5274 3622 Size: 81 1054 517 7061 Fragments arranged by size: 7061 1054 517 81 PshAI GACnn'nnGTC Cuts at: 1613 3684 1613 Size: 2071 6642 PsiI TTA'TAA Cuts at: 6283 6283 Size: 8713 PspOMI G'GGCC_C Cuts at: 3772 3916 4780 5042 3772 Size: 144 864 262 7443 Fragments arranged by size: 7443 864 262 144 PstI C_TGCA'G Cuts at: 830 2035 5273 6065 830 Size: 1205 3238 792 3478 Fragments arranged by size: 3478 3238 1205 792 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8713 PvuII CAG'CTG Cuts at: 2236 3019 5038 5400 5425 2236 Size: 783 2019 362 25 5524 Fragments arranged by size: 5524 2019 783 362 25 RsrII CG'GwC_CG Cuts at: 3867 3867 Size: 8713 SacI G_AGCT'C Cuts at: 3823 4866 3823 Size: 1043 7670 SacII CC_GC'GG Cuts at: 2203 3786 4755 2203 Size: 1583 969 6161 Fragments arranged by size: 6161 1583 969 SalI G'TCGA_C Cuts at: 1936 6055 1936 Size: 4119 4594 SanDI GG'GwC_CC Cuts at: 3703 4757 3703 Size: 1054 7659 SbfI CC_TGCA'GG Cuts at: 6065 6065 Size: 8713 ScaI AGT'ACT Cuts at: 2690 6961 8673 2690 Size: 4271 1712 2730 Fragments arranged by size: 4271 2730 1712 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 8713 SfcI C'TryA_G Cuts at: 826 1045 2031 2961 5269 6061 7196 7877 8068 Size: 219 986 930 2308 792 1135 681 191 Cuts at: 8068 826 Size: 1471 Fragments arranged by size: 2308 1471 1135 986 930 792 681 219 191 SfoI GGC'GCC Cuts at: 3830 4323 4704 3830 Size: 493 381 7839 Fragments arranged by size: 7839 493 381 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8713 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8713 SmaI CCC'GGG Cuts at: 1872 3922 4968 5021 6040 1872 Size: 2050 1046 53 1019 4545 Fragments arranged by size: 4545 2050 1046 1019 53 SmlI C'TyrA_G Cuts at: 820 1017 1943 4103 4247 6817 7718 7980 820 Size: 197 926 2160 144 2570 901 262 1553 Fragments arranged by size: 2570 2160 1553 926 901 262 197 144 SnaBI TAC'GTA Cuts at: 493 2293 6504 493 Size: 1800 4211 2702 Fragments arranged by size: 4211 2702 1800 SpeI A'CTAG_T Cuts at: 152 152 Size: 8713 SphI G_CATG'C Cuts at: 1132 5263 6071 1132 Size: 4131 808 3774 Fragments arranged by size: 4131 3774 808 SspI AAT'ATT Cuts at: 5 52 6637 5 Size: 47 6585 2081 Fragments arranged by size: 6585 2081 47 StuI AGG'CCT Cuts at: 1517 3245 3857 5670 1517 Size: 1728 612 1813 4560 Fragments arranged by size: 4560 1813 1728 612 StyI C'CwwG_G Cuts at: 513 1065 2004 2262 3759 5925 6152 513 Size: 552 939 258 1497 2166 227 3074 Fragments arranged by size: 3074 2166 1497 939 552 258 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2453 2587 2824 3325 3355 3884 5539 Size: 1255 873 134 237 501 30 529 1655 Cuts at: 5539 6740 6757 6910 7095 7540 325 Size: 1201 17 153 185 445 1498 Fragments arranged by size: 1655 1498 1255 1201 873 529 501 445 237 185 153 134 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2514 2688 3117 Size: 274 80 33 51 1443 537 174 429 Cuts at: 3117 6959 8671 96 Size: 3842 1712 138 Fragments arranged by size: 3842 1712 1443 537 429 274 174 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2594 2626 2862 2894 3478 3510 4297 4329 4721 Size: 32 236 32 584 32 787 32 392 Cuts at: 4721 4753 5531 5563 8673 8705 2594 Size: 32 778 32 3110 32 2602 Fragments arranged by size: 3110 2602 787 778 584 392 236 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3421 1316 Size: 2105 6608 XbaI T'CTAG_A Cuts at: 6049 6049 Size: 8713 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2735 3766 4439 2735 Size: 1031 673 7009 Fragments arranged by size: 7009 1031 673 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8713 XmaI C'CCGG_G Cuts at: 1870 3920 4966 5019 6038 1870 Size: 2050 1046 53 1019 4545 Fragments arranged by size: 4545 2050 1046 1019 53 XmnI GAAnn'nnTTC Cuts at: 3908 6842 3908 Size: 2934 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2386 6521 276 Size: 53 83 186 936 852 4135 2468 Fragments arranged by size: 4135 2468 936 852 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI SapI SfiI SrfI SwaI