(Linear) (Six-base) MAPSORT of: KIEE9633.seq Check: 7177 from: 1 to: 4008 KIEE9633.orf With 182 enzymes: SgfI * January 16, 2014 17:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1398 4008 Size: 1398 2610 AatII G_ACGT'C Cuts at: 0 383 4008 Size: 383 3625 AccI GT'mk_AC Cuts at: 0 578 947 959 1463 4008 Size: 578 369 12 504 2545 Fragments arranged by size: 2545 578 504 369 12 Acc65I G'GTAC_C Cuts at: 0 752 4008 Size: 752 3256 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 640 919 1561 1675 2973 3204 4008 Size: 186 454 279 642 114 1298 231 804 Fragments arranged by size: 1298 804 642 454 279 231 186 114 AfeI AGC'GCT Cuts at: 0 1129 4008 Size: 1129 2879 AflII C'TTAA_G Cuts at: 0 2098 4008 Size: 2098 1910 AflIII A'CryG_T Cuts at: 0 602 1109 4008 Size: 602 507 2899 Fragments arranged by size: 2899 602 507 AhdI GACnn_n'nnGTC Cuts at: 0 3376 4008 Size: 3376 632 AleI CACnn'nnGTG Cuts at: 0 1978 4008 Size: 1978 2030 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2303 4008 Size: 2303 1705 AlwNI CAG_nnn'CTG Cuts at: 0 618 2433 2528 3272 3740 3855 4008 Size: 618 1815 95 744 468 115 153 Fragments arranged by size: 1815 744 618 468 153 115 95 ApaI G_GGCC'C Cuts at: 0 1771 1915 2779 3041 4008 Size: 1771 144 864 262 967 Fragments arranged by size: 1771 967 864 262 144 ApaLI G'TGCA_C Cuts at: 0 250 2392 4008 Size: 250 2142 1616 Fragments arranged by size: 2142 1616 250 ApoI r'AATT_y Cuts at: 0 106 1057 4008 Size: 106 951 2951 Fragments arranged by size: 2951 951 106 AvaI C'yCGr_G Cuts at: 0 487 1179 1369 1603 1915 2677 2692 2961 Size: 487 692 190 234 312 762 15 269 Cuts at: 2961 3014 3797 4008 Size: 53 783 211 Fragments arranged by size: 783 762 692 487 312 269 234 211 190 53 15 BamHI G'GATC_C Cuts at: 0 935 4008 Size: 935 3073 BanI G'GyrC_C Cuts at: 0 752 1302 1823 2316 2352 2697 3007 3026 Size: 752 550 521 493 36 345 310 19 Cuts at: 3026 3787 4008 Size: 761 221 Fragments arranged by size: 761 752 550 521 493 345 310 221 36 19 BanII G_rGCy'C Cuts at: 0 1771 1818 1915 2779 2861 2951 3002 3041 Size: 1771 47 97 864 82 90 51 39 Cuts at: 3041 3452 4008 Size: 411 556 Fragments arranged by size: 1771 864 556 411 97 90 82 51 47 39 BbeI G_GCGC'C Cuts at: 0 1827 2320 2701 4008 Size: 1827 493 381 1307 Fragments arranged by size: 1827 1307 493 381 BbsI GAAGACnn'nnnn_ Cuts at: 0 1729 2018 3210 4008 Size: 1729 289 1192 798 Fragments arranged by size: 1729 1192 798 289 BbvCI CC'TCA_GC Cuts at: 0 2521 3229 3391 4008 Size: 2521 708 162 617 Fragments arranged by size: 2521 708 617 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2334 2368 4008 Size: 2334 34 1640 Fragments arranged by size: 2334 1640 34 BciVI GTATCCnnnnn_n' Cuts at: 0 3115 4008 Size: 3115 893 BclI T'GATC_A Cuts at: 0 1245 3846 4008 Size: 1245 2601 162 Fragments arranged by size: 2601 1245 162 BglI GCCn_nnn'nGGC Cuts at: 0 2658 2955 3662 4008 Size: 2658 297 707 346 Fragments arranged by size: 2658 707 346 297 BlpI GC'TnA_GC Cuts at: 0 2836 2848 3421 4008 Size: 2836 12 573 587 Fragments arranged by size: 2836 587 573 12 Bme1580I G_kGCm'C Cuts at: 0 254 445 1007 1305 1771 1915 2396 2779 Size: 254 191 562 298 466 144 481 383 Cuts at: 2779 3041 4008 Size: 262 967 Fragments arranged by size: 967 562 481 466 383 298 262 254 191 144 BmgBI CAC'GTC Cuts at: 0 174 417 2391 3189 3750 4008 Size: 174 243 1974 798 561 258 Fragments arranged by size: 1974 798 561 258 243 174 BmrI ACTGGGnnnn_n' Cuts at: 0 349 456 600 1703 4008 Size: 349 107 144 1103 2305 Fragments arranged by size: 2305 1103 349 144 107 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 317 319 2522 4008 Size: 317 2 2203 1486 Fragments arranged by size: 2203 1486 317 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 568 1120 1243 1654 2718 3492 4008 Size: 229 339 552 123 411 1064 774 516 Fragments arranged by size: 1064 774 552 516 411 339 229 123 Bpu10I CC'TnA_GC Cuts at: 0 2521 3229 3391 4008 Size: 2521 708 162 617 Fragments arranged by size: 2521 708 617 162 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2227 4008 Size: 2227 1781 BsaI GGTCTCn'nnnn_ Cuts at: 0 860 1509 3495 4008 Size: 860 649 1986 513 Fragments arranged by size: 1986 860 649 513 BsaAI yAC'GTr Cuts at: 0 288 1110 4008 Size: 288 822 2898 Fragments arranged by size: 2898 822 288 BsaHI Gr'CG_yC Cuts at: 0 380 484 1824 2317 2698 4008 Size: 380 104 1340 493 381 1310 Fragments arranged by size: 1340 1310 493 381 380 104 BsaWI w'CCGG_w Cuts at: 0 928 1799 3524 3635 4008 Size: 928 871 1725 111 373 Fragments arranged by size: 1725 928 871 373 111 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 857 887 1473 1503 2292 2322 2526 2556 Size: 857 30 586 30 789 30 204 30 Cuts at: 2556 2716 2746 4008 Size: 160 30 1262 Fragments arranged by size: 1262 857 789 586 204 160 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 193 226 838 1246 1249 1276 1279 1282 Size: 193 33 612 408 3 27 3 3 Cuts at: 1282 1294 2041 2313 2326 2472 2797 2959 4008 Size: 12 747 272 13 146 325 162 1049 Fragments arranged by size: 1049 747 612 408 325 272 193 162 146 33 27 13 12 3 3 3 BseYI C'CCAG_C Cuts at: 0 494 611 1124 1189 1764 2147 2755 3033 Size: 494 117 513 65 575 383 608 278 Cuts at: 3033 3040 4008 Size: 7 968 Fragments arranged by size: 968 608 575 513 494 383 278 117 65 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 973 1069 4008 Size: 787 186 96 2939 Fragments arranged by size: 2939 787 186 96 BsiHKAI G_wGCw'C Cuts at: 0 254 554 1664 1818 2065 2342 2396 2861 Size: 254 300 1110 154 247 277 54 465 Cuts at: 2861 4008 Size: 1147 Fragments arranged by size: 1147 1110 465 300 277 254 247 154 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 371 475 3384 4008 Size: 371 104 2909 624 Fragments arranged by size: 2909 624 371 104 Bsp1286I G_dGCh'C Cuts at: 0 254 445 554 1007 1305 1664 1771 1818 Size: 254 191 109 453 298 359 107 47 Cuts at: 1818 1915 2065 2342 2396 2779 2861 2951 3002 Size: 97 150 277 54 383 82 90 51 Cuts at: 3002 3041 3452 4008 Size: 39 411 556 Fragments arranged by size: 556 453 411 383 359 298 277 254 191 150 109 107 97 90 82 54 51 47 39 BspEI T'CCGG_A Cuts at: 0 3524 3635 4008 Size: 3524 111 373 Fragments arranged by size: 3524 373 111 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1398 2234 2295 2318 3779 4008 Size: 1398 836 61 23 1461 229 Fragments arranged by size: 1461 1398 836 229 61 23 BsrBI CCG'CTC Cuts at: 0 2732 4008 Size: 2732 1276 BsrDI GCAATG_nn' Cuts at: 0 650 2079 4008 Size: 650 1429 1929 Fragments arranged by size: 1929 1429 650 BsrFI r'CCGG_y Cuts at: 0 401 1483 2173 3437 4008 Size: 401 1082 690 1264 571 Fragments arranged by size: 1264 1082 690 571 401 BsrGI T'GTAC_A Cuts at: 0 509 4008 Size: 509 3499 BssSI C'ACGA_G Cuts at: 0 2866 4008 Size: 2866 1142 BstXI CCAn_nnnn'nTGG Cuts at: 0 1455 2147 4008 Size: 1455 692 1861 Fragments arranged by size: 1861 1455 692 BstYI r'GATC_y Cuts at: 0 22 935 1019 2536 2870 3576 4008 Size: 22 913 84 1517 334 706 432 Fragments arranged by size: 1517 913 706 432 334 84 22 Bsu36I CC'TnA_GG Cuts at: 0 1256 2398 3898 4008 Size: 1256 1142 1500 110 Fragments arranged by size: 1500 1256 1142 110 BtgI C'CryG_G Cuts at: 0 195 257 1496 1778 2747 4008 Size: 195 62 1239 282 969 1261 Fragments arranged by size: 1261 1239 969 282 195 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1848 1938 2441 4008 Size: 1848 90 503 1567 Fragments arranged by size: 1848 1567 503 90 BtsI GCAGTG_nn' Cuts at: 0 1954 3324 3776 4008 Size: 1954 1370 452 232 Fragments arranged by size: 1954 1370 452 232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3855 3890 4008 Size: 3855 35 118 Fragments arranged by size: 3855 118 35 EaeI y'GGCC_r Cuts at: 0 116 1457 1685 1714 1790 2642 2894 3130 Size: 116 1341 228 29 76 852 252 236 Cuts at: 3130 3212 4008 Size: 82 796 Fragments arranged by size: 1341 852 796 252 236 228 116 82 76 29 EarI CTCTTCn'nnn_ Cuts at: 0 1212 2018 4008 Size: 1212 806 1990 Fragments arranged by size: 1990 1212 806 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 992 3691 4008 Size: 992 2699 317 Fragments arranged by size: 2699 992 317 EcoICRI GAG'CTC Cuts at: 0 1816 2859 4008 Size: 1816 1043 1149 Fragments arranged by size: 1816 1149 1043 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 229 568 640 919 1120 1243 1561 Size: 186 43 339 72 279 201 123 318 Cuts at: 1561 1654 1675 2718 2973 3204 3492 4008 Size: 93 21 1043 255 231 288 516 Fragments arranged by size: 1043 516 339 318 288 279 255 231 201 186 123 93 72 43 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1141 2242 4008 Size: 1141 1101 1766 Fragments arranged by size: 1766 1141 1101 EcoO109I rG'GnC_Cy Cuts at: 0 1365 1545 1617 1698 1911 1912 2501 2752 Size: 1365 180 72 81 213 1 589 251 Cuts at: 2752 2775 3037 3269 4008 Size: 23 262 232 739 Fragments arranged by size: 1365 739 589 262 251 232 213 180 81 72 23 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2605 4008 Size: 2605 1403 FspI TGC'GCA Cuts at: 0 2374 3049 4008 Size: 2374 675 959 Fragments arranged by size: 2374 959 675 HaeII r_GCGC'y Cuts at: 0 129 817 1131 1827 2320 2641 2664 2701 Size: 129 688 314 696 493 321 23 37 Cuts at: 2701 3120 4008 Size: 419 888 Fragments arranged by size: 888 696 688 493 419 321 314 129 37 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 151 183 1189 1221 2326 2358 2526 2528 Size: 151 32 1006 32 1105 32 168 2 Cuts at: 2528 2558 2560 3362 3394 3568 3600 3606 3638 Size: 30 2 802 32 174 32 6 32 Cuts at: 3638 4008 Size: 370 Fragments arranged by size: 1105 1006 802 370 174 168 151 32 32 32 32 32 32 30 6 2 2 HincII GTy'rAC Cuts at: 0 2127 4008 Size: 2127 1881 HindIII A'AGCT_T Cuts at: 0 217 2587 4008 Size: 217 2370 1421 Fragments arranged by size: 2370 1421 217 KasI G'GCGC_C Cuts at: 0 1823 2316 2697 4008 Size: 1823 493 381 1311 Fragments arranged by size: 1823 1311 493 381 KpnI G_GTAC'C Cuts at: 0 756 4008 Size: 756 3252 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 149 592 820 913 1409 2246 2312 2903 Size: 149 443 228 93 496 837 66 591 Cuts at: 2903 4008 Size: 1105 Fragments arranged by size: 1105 837 591 496 443 228 149 93 66 MscI TGG'CCA Cuts at: 0 1687 1716 2644 2896 4008 Size: 1687 29 928 252 1112 Fragments arranged by size: 1687 1112 928 252 29 MslI CAynn'nnrTG Cuts at: 0 448 1667 1978 2053 4008 Size: 448 1219 311 75 1955 Fragments arranged by size: 1955 1219 448 311 75 MspA1I CmG'CkG Cuts at: 0 197 231 498 1014 1780 2749 3033 3281 Size: 197 34 267 516 766 969 284 248 Cuts at: 3281 3395 3420 3519 4008 Size: 114 25 99 489 Fragments arranged by size: 969 766 516 489 284 267 248 197 114 99 34 25 NaeI GCC'GGC Cuts at: 0 403 2175 4008 Size: 403 1772 1833 Fragments arranged by size: 1833 1772 403 NarI GG'CG_CC Cuts at: 0 1824 2317 2698 4008 Size: 1824 493 381 1310 Fragments arranged by size: 1824 1310 493 381 NcoI C'CATG_G Cuts at: 0 257 4008 Size: 257 3751 NgoMIV G'CCGG_C Cuts at: 0 401 2173 4008 Size: 401 1772 1835 Fragments arranged by size: 1835 1772 401 NspI r_CATG'y Cuts at: 0 58 459 606 3258 3711 4008 Size: 58 401 147 2652 453 297 Fragments arranged by size: 2652 453 401 297 147 58 PciI A'CATG_T Cuts at: 0 602 4008 Size: 602 3406 PflMI CCAn_nnn'nTGG Cuts at: 0 3546 4008 Size: 3546 462 PfoI T'CCnGG_A Cuts at: 0 1097 1580 1631 2611 3452 4008 Size: 1097 483 51 980 841 556 Fragments arranged by size: 1097 980 841 556 483 51 PpuMI rG'GwC_Cy Cuts at: 0 1617 1698 2752 3269 4008 Size: 1617 81 1054 517 739 Fragments arranged by size: 1617 1054 739 517 81 PshAI GACnn'nnGTC Cuts at: 0 1679 4008 Size: 1679 2329 PspOMI G'GGCC_C Cuts at: 0 1767 1911 2775 3037 4008 Size: 1767 144 864 262 971 Fragments arranged by size: 1767 971 864 262 144 PstI C_TGCA'G Cuts at: 0 30 3268 4008 Size: 30 3238 740 Fragments arranged by size: 3238 740 30 PvuII CAG'CTG Cuts at: 0 231 1014 3033 3395 3420 4008 Size: 231 783 2019 362 25 588 Fragments arranged by size: 2019 783 588 362 231 25 RsrII CG'GwC_CG Cuts at: 0 1862 4008 Size: 1862 2146 SacI G_AGCT'C Cuts at: 0 1818 2861 4008 Size: 1818 1043 1147 Fragments arranged by size: 1818 1147 1043 SacII CC_GC'GG Cuts at: 0 198 1781 2750 4008 Size: 198 1583 969 1258 Fragments arranged by size: 1583 1258 969 198 SanDI GG'GwC_CC Cuts at: 0 1698 2752 4008 Size: 1698 1054 1256 Fragments arranged by size: 1698 1256 1054 ScaI AGT'ACT Cuts at: 0 685 4008 Size: 685 3323 SexAI A'CCwGG_T Cuts at: 0 581 4008 Size: 581 3427 SfcI C'TryA_G Cuts at: 0 26 956 3264 4008 Size: 26 930 2308 744 Fragments arranged by size: 2308 930 744 26 SfoI GGC'GCC Cuts at: 0 1825 2318 2699 4008 Size: 1825 493 381 1309 Fragments arranged by size: 1825 1309 493 381 SmaI CCC'GGG Cuts at: 0 1917 2963 3016 4008 Size: 1917 1046 53 992 Fragments arranged by size: 1917 1046 992 53 SmlI C'TyrA_G Cuts at: 0 2098 2242 4008 Size: 2098 144 1766 Fragments arranged by size: 2098 1766 144 SnaBI TAC'GTA Cuts at: 0 288 4008 Size: 288 3720 SphI G_CATG'C Cuts at: 0 3258 4008 Size: 3258 750 StuI AGG'CCT Cuts at: 0 1240 1852 3665 4008 Size: 1240 612 1813 343 Fragments arranged by size: 1813 1240 612 343 StyI C'CwwG_G Cuts at: 0 257 1754 3920 4008 Size: 257 1497 2166 88 Fragments arranged by size: 2166 1497 257 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 448 582 819 1320 1350 1879 3534 4008 Size: 448 134 237 501 30 529 1655 474 Fragments arranged by size: 1655 529 501 474 448 237 134 30 TatI w'GTAC_w Cuts at: 0 509 683 1112 4008 Size: 509 174 429 2896 Fragments arranged by size: 2896 509 429 174 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 589 621 857 889 1473 1505 2292 2324 Size: 589 32 236 32 584 32 787 32 Cuts at: 2324 2716 2748 3526 3558 4008 Size: 392 32 778 32 450 Fragments arranged by size: 787 778 589 584 450 392 236 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1416 4008 Size: 1416 2592 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 730 1761 2434 4008 Size: 730 1031 673 1574 Fragments arranged by size: 1574 1031 730 673 XmaI C'CCGG_G Cuts at: 0 1915 2961 3014 4008 Size: 1915 1046 53 994 Fragments arranged by size: 1915 1046 994 53 XmnI GAAnn'nnTTC Cuts at: 0 1903 4008 Size: 1903 2105 ZraI GAC'GTC Cuts at: 0 381 4008 Size: 381 3627 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AfeI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII RsrII SacI SacII SanDI ScaI SexAI SfcI SfoI SmaI SmlI SnaBI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AclI AgeI AloI AscI AseI AsiSI AvrII BaeI BfrBI BglII BmtI BsaBI BsiEI BsiWI BsmI BspHI BssHII BstAPI BstBI BstEII BstZ17I ClaI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspAI HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PmeI PmlI PpiI PsiI PspXI PsrI PvuI SalI SapI SbfI SfiI SgfI SgrAI SpeI SrfI SspI SwaI XbaI XhoI