Restriction map for
pFN21AE6435
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI Eam1105I
EcoO109I EcoRV EcoT22I FauI FseI
FspI HgaI Hin1I HindIII HphI
KasI KpnI KspI MamI MluI
MroI MvnI NaeI NarI NcoI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tsp45I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 261 ACATGT A^C[GA][CT]GT
AlwNI 1228 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 127 CCTCAGG CC^T[ACGT]AGG
Aor51HI 137 AGCGCT AGC^GCT
AvaII 673 GGACC G^G[AT]CC
BalI 1384 TGGCCA TGG^CCA
BamHI 174 GGATCC G^GATCC
BanII 97 GAGCCC G[GA]GC[CT]^C
BclI 224 TGATCA T^GATCA
BcnI 84 CCGGG CC^[GC]GG
BglII 113 AGATCT A^GATCT
BmyI 97 GAGCCC G[AGT]GC[ACT]^C
BsaJI 531 CCTTGG C^C[ACGT][ACGT]GG
BseRI 64 CTCCTC GAGGAG(10/8)
BsgI 587 GTGCAG GTGCAG(16/14)
Bsp1286I 97 GAGCCC G[AGT]GC[ACT]^C
BspHI 843 TCATGA T^CATGA
BspLU11I 261 ACATGT A^CATGT
BsrDI 600 CATTGC GCAATG(2/0)
BsrI 168 CCAGT ACTGG(1/-1)
BstEII 984 GGTTACC G^GT[ACGT]ACC
BstPI 984 GGTTACC G^GT[ACGT]ACC
Bsu36I 127 CCTCAGG CC^T[ACGT]AGG
Cac8I 1041 GCTAGC GC[ACGT]^[ACGT]GC
DraI 730 TTTAAA TTT^AAA
EagI 29 CGGCCG C^GGCCG
EclXI 29 CGGCCG C^GGCCG
Eco47III 137 AGCGCT AGC^GCT
Eco52I 29 CGGCCG C^GGCCG
Eco81I 127 CCTCAGG CC^T[ACGT]AGG
EcoO65I 984 GGTTACC G^GT[ACGT]ACC
EcoT14I 531 CCTTGG C^C[AT][AT]GG
FbaI 224 TGATCA T^GATCA
HaeII 137 AGCGCT [GA]GCGC^[CT]
HapII 84 CCGG C^CGG
HpaI 612 GTTAAC GTT^AAC
HpaII 84 CCGG C^CGG
MaeII 963 ACGT A^CGT
MfeI 215 CAATTG C^AATTG
MluNI 1384 TGGCCA TGG^CCA
MscI 1384 TGGCCA TGG^CCA
MslI 276 CATTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 7 CAGCTG C[AC]G^C[GT]G
MspI 84 CCGG C^CGG
MunI 215 CAATTG C^AATTG
MwoI 126 GCCTCAGGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 84 CCGGG CC^[GC]GG
NdeI 1200 CATATG CA^TATG
NheI 1041 GCTAGC G^CTAGC
NlaIV 174 GGATCC GG[ACGT]^[ACGT]CC
NspI 261 ACATGT [GA]CATG^[CT]
PvuI 831 CGATCG CGAT^CG
PvuII 7 CAGCTG CAG^CTG
RcaI 843 TCATGA T^CATGA
SexAI 206 ACCTGGT A^CC[AT]GGT
SfaNI 282 GATGC GCATC(5/9)
SspI 1109 AATATT AAT^ATT
StyI 531 CCTTGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AciI 32 CCGC CCGC(-3/-1)
74 CCGC
BciVI 55 GTATCC GTATCC(6/5)
380 GTATCC
BsiEI 29 CGGCCG CG[GA][CT]^CG
831 CGATCG
BsmAI 921 GTCTC GTCTC(1/5)
994 GTCTC
BspMI 122 ACCTGC ACCTGC(4/8)
231 ACCTGC
BstNI 196 CCTGG CC^[AT]GG
207 CCTGG
EaeI 29 CGGCCG [CT]^GGCC[GA]
1384 TGGCCA
EarI 67 CTCTTC CTCTTC(1/4)
304 CTCTTC
EcoNI 123 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
664 CCTATTAAAGG
EcoRI 685 GAATTC G^AATTC
968 GAATTC
EcoRII 196 CCTGG ^CC[AT]GG
207 CCTGG
HincII 396 GTCAAC GT[CT]^[GA]AC
612 GTTAAC
HindII 396 GTCAAC GT[CT]^[GA]AC
612 GTTAAC
Ksp632I 67 CTCTTC CTCTTC(1/4)
304 CTCTTC
MvaI 196 CCTGG CC^[AT]GG
207 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpuAI BsiYI
BslI BssKI BstF5I BstYI CfoI
Cfr13I DdeI DpnII Fnu4HI FokI
HaeIII HhaI HinP1I HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI NdeII NlaIII PleI
RsaI Sau3AI Sau96I ScrFI SmlI
Tru9I TseI Tsp509I TspRI XhoII