(Circular) (Six-base) MAPSORT of: pFN21AE6435.seq Check: 392 from: 1 to: 6124 pFN21AE6435 6124 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2136 2136 Size: 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 3445 3445 Size: 6124 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2296 2511 2690 4187 5571 5839 1632 Size: 436 228 215 179 1497 1384 268 1917 Fragments arranged by size: 1917 1497 1384 436 268 228 215 179 AfeI AGC'GCT Cuts at: 2144 2144 Size: 6124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2266 5023 6000 1203 Size: 1063 2757 977 1327 Fragments arranged by size: 2757 1327 1063 977 AgeI A'CCGG_T Cuts at: 4955 4955 Size: 6124 AhdI GACnn_n'nnGTC Cuts at: 4853 4853 Size: 6124 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AlwNI CAG_nnn'CTG Cuts at: 3238 3867 5439 3238 Size: 629 1572 3923 Fragments arranged by size: 3923 1572 629 ApaLI G'TGCA_C Cuts at: 4181 5337 4181 Size: 1156 4968 ApoI r'AATT_y Cuts at: 1493 2690 2700 2973 3020 3190 3245 3433 3659 Size: 1197 10 273 47 170 55 188 226 Cuts at: 3659 3811 5920 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1197 273 226 188 170 152 55 47 10 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 3449 1870 Size: 73 1506 4545 Fragments arranged by size: 4545 1506 73 BamHI G'GATC_C Cuts at: 2179 3454 3821 2179 Size: 1275 367 4482 Fragments arranged by size: 4482 1275 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3445 4900 618 Size: 325 217 627 1658 1455 1842 Fragments arranged by size: 1842 1658 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2106 3443 1391 Size: 715 1337 4072 Fragments arranged by size: 4072 1337 715 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2578 2661 2852 928 Size: 971 679 83 191 4200 Fragments arranged by size: 4200 971 679 191 83 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4315 4349 1903 Size: 34 2378 34 3678 Fragments arranged by size: 3678 2378 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2071 2396 4016 5226 2071 Size: 325 1620 1210 2969 Fragments arranged by size: 2969 1620 1210 325 BclI T'GATC_A Cuts at: 1548 2229 1548 Size: 681 5443 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2118 6119 2118 Size: 4001 2123 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 4185 5341 1790 Size: 2395 1156 2573 Fragments arranged by size: 2573 2395 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2167 3855 4813 Size: 416 53 83 399 24 750 1688 958 Cuts at: 4813 442 Size: 1753 Fragments arranged by size: 1753 1688 958 750 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3050 1056 Size: 1994 4130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4784 1239 Size: 3545 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2062 2365 2764 4249 5114 5412 2062 Size: 303 399 1485 865 298 2774 Fragments arranged by size: 2774 1485 865 399 303 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4787 882 Size: 617 3288 2219 Fragments arranged by size: 3288 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3915 493 Size: 711 2711 2702 Fragments arranged by size: 2711 2702 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3931 4313 4948 275 Size: 53 83 186 936 2398 382 635 1451 Fragments arranged by size: 2398 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4557 4955 5229 5376 5787 4557 Size: 398 274 147 411 4894 Fragments arranged by size: 4894 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5899 Fragments arranged by size: 5899 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 1186 Size: 874 5250 BseYI C'CCAG_C Cuts at: 5327 5823 5327 Size: 496 5628 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2613 1163 Size: 573 877 4674 Fragments arranged by size: 4674 877 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2839 3574 4335 5363 1540 Size: 275 186 36 802 735 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 735 275 186 36 BsiHKAI G_wGCw'C Cuts at: 4185 4270 5341 4185 Size: 85 1071 4968 Fragments arranged by size: 4968 1071 85 BsmI GAATG_Cn' Cuts at: 3635 3728 3635 Size: 93 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 3443 4185 4270 5341 1391 Size: 399 316 1337 742 85 1071 2174 Fragments arranged by size: 2174 1337 1071 742 399 316 85 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 2848 4013 5743 2848 Size: 1165 1730 3229 Fragments arranged by size: 3229 1730 1165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2136 2245 3479 844 Size: 1292 109 1234 3489 Fragments arranged by size: 3489 1292 1234 109 BsrBI CCG'CTC Cuts at: 1394 1823 4011 1394 Size: 429 2188 3507 Fragments arranged by size: 3507 2188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2604 4619 4793 65 Size: 1183 1356 2015 174 1396 Fragments arranged by size: 2015 1396 1356 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4768 4955 1700 Size: 110 2958 187 2869 Fragments arranged by size: 2958 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6124 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 4184 5196 4184 Size: 1012 5112 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 2989 1184 Size: 1805 4319 BstYI r'GATC_y Cuts at: 1972 2118 2179 2247 2468 3454 3821 4206 4223 Size: 146 61 68 221 986 367 385 17 Cuts at: 4223 5675 5761 5773 6119 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 986 385 367 346 221 146 86 68 61 17 12 Bsu36I CC'TnA_GG Cuts at: 2133 2133 Size: 6124 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4633 Fragments arranged by size: 4633 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 3640 4434 4454 3640 Size: 794 20 5310 Fragments arranged by size: 5310 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6089 DraI TTT'AAA Cuts at: 2737 3428 2737 Size: 691 5433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3389 3571 Size: 54 1425 50 275 18 204 1355 182 Cuts at: 3571 4460 6089 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1355 889 275 204 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 3571 1537 Size: 275 222 1537 4090 Fragments arranged by size: 4090 1537 275 222 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 2315 4054 1353 Size: 725 237 1739 3423 Fragments arranged by size: 3423 1739 725 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2068 4711 5085 5231 387 Size: 1064 138 479 2643 374 146 1280 Fragments arranged by size: 2643 1280 1064 479 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2296 2511 2690 4187 4784 5571 Size: 393 436 228 215 179 1497 597 787 Cuts at: 5571 5839 1239 Size: 268 1524 Fragments arranged by size: 1524 1497 787 597 436 393 268 228 215 179 EcoNI CCTnn'n_nnAGG Cuts at: 2132 2673 2132 Size: 541 5583 EcoRI G'AATT_C Cuts at: 2690 2973 3433 3811 2690 Size: 283 460 378 5003 Fragments arranged by size: 5003 460 378 283 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2678 2876 2678 Size: 198 5926 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 2146 5271 1678 Size: 468 3125 2531 Fragments arranged by size: 3125 2531 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4765 4797 4839 4871 1373 Size: 32 135 32 3193 32 42 32 2626 Fragments arranged by size: 3193 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2403 2619 3468 3714 669 Size: 988 281 465 216 849 246 3079 Fragments arranged by size: 3079 988 849 465 281 246 216 HindIII A'AGCT_T Cuts at: 748 748 Size: 6124 HpaI GTT'AAC Cuts at: 2619 3714 2619 Size: 1095 5029 KpnI G_GTAC'C Cuts at: 3449 3449 Size: 6124 MfeI C'AATT_G Cuts at: 2220 3723 2220 Size: 1503 4621 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2450 3128 5238 5422 1305 Size: 1145 678 2110 184 2007 Fragments arranged by size: 2110 2007 1145 678 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3391 10 Size: 54 1425 343 1559 2743 Fragments arranged by size: 2743 1559 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 4082 4441 4600 518 Size: 648 1119 1797 359 159 2042 Fragments arranged by size: 2042 1797 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2014 3540 4217 5365 5610 1606 Size: 408 1526 677 1148 245 2120 Fragments arranged by size: 2120 1526 1148 677 408 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4633 Fragments arranged by size: 4633 939 552 NdeI CA'TA_TG Cuts at: 387 3206 387 Size: 2819 3305 NheI G'CTAG_C Cuts at: 1052 3046 1052 Size: 1994 4130 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2270 3482 5027 1132 Size: 97 1041 1212 1545 2229 Fragments arranged by size: 2229 1545 1212 1041 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 2266 5023 2266 Size: 2757 3367 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4193 4225 5706 5738 4193 Size: 32 1481 32 4579 Fragments arranged by size: 4579 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PstI C_TGCA'G Cuts at: 830 3476 830 Size: 2646 3478 PvuI CG_AT'CG Cuts at: 2001 2839 2001 Size: 838 5286 PvuII CAG'CTG Cuts at: 2014 2014 Size: 6124 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SexAI A'CCwGG_T Cuts at: 2211 2211 Size: 6124 SfcI C'TryA_G Cuts at: 826 1045 3472 4607 5288 5479 826 Size: 219 2427 1135 681 191 1471 Fragments arranged by size: 2427 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2041 2380 2743 4228 5129 5391 Size: 197 926 98 339 363 1485 901 262 Cuts at: 5391 820 Size: 1553 Fragments arranged by size: 1553 1485 926 901 363 339 262 197 98 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 3482 1132 Size: 2350 3774 SspI AAT'ATT Cuts at: 5 52 3116 4048 5 Size: 47 3064 932 2081 Fragments arranged by size: 3064 2081 932 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6124 StyI C'CwwG_G Cuts at: 513 1065 2004 2536 3563 513 Size: 552 939 532 1027 3074 Fragments arranged by size: 3074 1027 939 552 532 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4151 4168 4321 4506 4951 325 Size: 1255 2571 17 153 185 445 1498 Fragments arranged by size: 2571 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4370 6082 96 Size: 274 80 33 51 1443 2393 1712 138 Fragments arranged by size: 2393 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6084 6116 6084 Size: 32 6092 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 4253 4253 Size: 6124 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI