Restriction map for
pFN21AB3993
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DpnII
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeI MamI MboI
MfeI MflI MluI MroI MunI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SalI SapI Sau3AI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI StuI SwaI Tth111I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcyI 52 GGCGCC G[GA]^CG[CT]C
Alw44I 1150 GTGCAC G^TGCAC
ApaLI 1150 GTGCAC G^TGCAC
BalI 662 TGGCCA TGG^CCA
BbeI 52 GGCGCC GGCGC^C
BglI 590 GCCTCATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 160 CTGGAG CTGGAG(16/14)
BsaAI 697 TACGTG [CT]AC^GT[GA]
BsaHI 52 GGCGCC G[GA]^CG[CT]C
BsgI 835 CTGCAC GTGCAG(16/14)
BsrBI 1171 GAGCGG CCGCTC(-3/-3)
BssSI 718 CACGAG CACGAG(-5/-1)
BstAPI 1176 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 665 CCATGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoRV 745 GATATC GAT^ATC
HgaI 145 GCGTC GACGC(5/10)
Hin1I 52 GGCGCC G[GA]^CG[CT]C
KasI 52 GGCGCC G^GCGCC
KspI 56 CCGCGG CCGC^GG
MaeII 698 ACGT A^CGT
MluNI 662 TGGCCA TGG^CCA
MscI 662 TGGCCA TGG^CCA
MseI 299 TTAA T^TAA
MslI 735 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 52 GGCGCC GG^CGCC
PflMI 309 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 279 GAGTC GAGTC(4/5)
PpuMI 602 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 274 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 532 GAGCTC GAGCT^C
SacII 56 CCGCGG CCGC^GG
ScaI 1211 AGTACT AGT^ACT
SfoI 52 GGCGCC GGC^GCC
SmlI 976 CTTGAG C^T[CT][GA]AG
SspI 566 AATATT AAT^ATT
TfiI 948 GAATC G^A[AT]TC
Tru9I 299 TTAA T^TAA
Tsp45I 1101 GTCAC ^GT[GC]AC
Van91I 309 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 34 GAATTC [GA]^AATT[CT]
921 GAATTC
AfaI 1191 GTAC GT^AC
1212 GTAC
AluI 533 AGCT AG^CT
641 AGCT
ApoI 34 GAATTC [GA]^AATT[CT]
921 GAATTC
AspHI 532 GAGCTC G[AT]GC[AT]^C
1150 GTGCAC
BsaI 979 GAGACC GGTCTC(1/5)
1058 GAGACC
BseRI 1126 CTCCTC GAGGAG(10/8)
721 GAGGAG
BsiHKAI 532 GAGCTC G[AT]GC[AT]^C
1150 GTGCAC
BsmAI 979 GAGAC GTCTC(1/5)
1058 GAGAC
BsrFI 237 GCCGGC [GA]^CCGG[CT]
1217 GCCGGC
BssHII 124 GCGCGC G^CGCGC
241 GCGCGC
Cfr10I 237 GCCGGC [GA]^CCGG[CT]
1217 GCCGGC
DdeI 11 CTCAG C^T[ACGT]AG
495 CTCAG
EaeI 251 TGGCCG [CT]^GGCC[GA]
662 TGGCCA
EcoRI 34 GAATTC G^AATTC
921 GAATTC
HinfI 279 GAGTC G^A[ACGT]TC
948 GAATC
MaeIII 899 GTTAC ^GT[ACGT]AC
1101 GTCAC
NaeI 237 GCCGGC GCC^GGC
1217 GCCGGC
NcoI 740 CCATGG C^CATGG
863 CCATGG
NgoMIV 237 GCCGGC G^CCGGC
1217 GCCGGC
RsaI 1191 GTAC GT^AC
1212 GTAC
SbfI 213 CCTGCAGG CCTGCA^GG
816 CCTGCAGG
SfaNI 706 GATGC GCATC(5/9)
1235 GCATC
Sse8387I 213 CCTGCAGG CCTGCA^GG
816 CCTGCAGG
TaqI 557 TCGA T^CGA
645 TCGA
TspRI 659 CACTG CA[GC]TG[ACGT][ACGT]^
1177 CAGTG
TthHB8I 557 TCGA T^CGA
645 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AlwNI ApaI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI SmaI StyI TseI Tsp509I
XmaCI XmaI