(Circular) (Six-base) MAPSORT of: pFN21AB3993.seq Check: 1872 from: 1 to: 5959 pFN21AB3993 5959 bp With 182 enzymes: SgfI * November 16, 2017 15:15 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2109 3120 2109 Size: 1011 4948 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3302 1937 Size: 1365 4594 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2419 4022 5406 5674 1632 Size: 787 1603 1384 268 1917 Fragments arranged by size: 1917 1603 1384 787 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 2320 2836 2857 3187 3702 5274 2320 Size: 516 21 330 515 1572 3005 Fragments arranged by size: 3005 1572 516 515 330 21 ApaI G_GGCC'C Cuts at: 2024 2032 2052 2126 2024 Size: 8 20 74 5857 Fragments arranged by size: 5857 74 20 8 ApaLI G'TGCA_C Cuts at: 3155 4016 5172 3155 Size: 861 1156 3942 Fragments arranged by size: 3942 1156 861 ApoI r'AATT_y Cuts at: 1493 2039 2926 3268 3494 3646 5755 1493 Size: 546 887 342 226 152 2109 1697 Fragments arranged by size: 2109 1697 887 546 342 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2025 2159 3168 3284 1870 Size: 73 82 134 1009 116 4545 Fragments arranged by size: 4545 1009 134 116 82 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2533 2566 2533 Size: 33 5926 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2485 2803 3280 4735 Size: 325 217 627 270 428 318 477 1455 Cuts at: 4735 618 Size: 1842 Fragments arranged by size: 1842 1455 627 477 428 325 318 270 217 BanII G_rGCy'C Cuts at: 1391 2024 2032 2052 2126 2183 2541 3278 1391 Size: 633 8 20 74 57 358 737 4072 Fragments arranged by size: 4072 737 633 358 74 57 20 8 BbeI G_GCGC'C Cuts at: 2061 2061 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4988 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2601 4570 136 Size: 107 122 71 631 605 929 1969 1525 Fragments arranged by size: 1969 1525 929 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 2024 2032 2052 2126 2808 3159 4020 5176 Size: 234 8 20 74 682 351 861 1156 Cuts at: 5176 1790 Size: 2573 Fragments arranged by size: 2573 1156 861 682 351 234 74 20 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 2824 3690 Size: 416 53 83 399 24 1048 359 866 Cuts at: 3690 4648 442 Size: 958 1753 Fragments arranged by size: 1753 1048 958 866 416 399 359 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 4619 1239 Size: 947 2433 2579 Fragments arranged by size: 2579 2433 947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3002 4084 4949 5247 3002 Size: 1082 865 298 3714 Fragments arranged by size: 3714 1082 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2978 3057 4622 882 Size: 617 1479 79 1565 2219 Fragments arranged by size: 2219 1565 1479 617 79 BsaAI yAC'GTr Cuts at: 493 1204 2704 3750 493 Size: 711 1500 1046 2702 Fragments arranged by size: 2702 1500 1046 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 3766 4148 4783 Size: 53 83 186 936 525 1708 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1708 1451 936 635 525 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2159 2189 1178 Size: 30 165 30 756 30 4948 Fragments arranged by size: 4948 756 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2741 3122 1186 Size: 1555 381 4023 Fragments arranged by size: 4023 1555 381 BseYI C'CCAG_C Cuts at: 2137 2347 2604 2975 5162 5658 2137 Size: 210 257 371 2187 496 2438 Fragments arranged by size: 2438 2187 496 371 257 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 4297 Fragments arranged by size: 4297 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2541 3159 4020 4105 5176 2541 Size: 618 861 85 1071 3324 Fragments arranged by size: 3324 1071 861 618 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2032 2052 2126 2183 2541 2808 Size: 399 234 8 20 74 57 358 267 Cuts at: 2808 3159 3278 4020 4105 5176 1391 Size: 351 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 351 267 234 119 85 74 57 20 8 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2452 3120 3314 844 Size: 1265 343 668 194 3489 Fragments arranged by size: 3489 1265 668 343 194 BsrBI CCG'CTC Cuts at: 1394 1823 3178 3846 1394 Size: 429 1355 668 3507 Fragments arranged by size: 3507 1355 668 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2242 3222 4603 4790 1700 Size: 110 432 980 1381 187 2869 Fragments arranged by size: 2869 1381 980 432 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 2129 2246 1925 Size: 204 117 5638 Fragments arranged by size: 5638 204 117 BssSI C'ACGA_G Cuts at: 2723 4019 5031 2723 Size: 1296 1012 3651 Fragments arranged by size: 3651 1296 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3187 794 Size: 2393 3566 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstXI CCAn_nnnn'nTGG Cuts at: 2677 2677 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 2183 2745 2868 2917 513 Size: 552 939 57 122 562 123 49 3555 Fragments arranged by size: 3555 939 562 552 123 122 57 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3188 3475 4269 4289 3188 Size: 287 794 20 4858 Fragments arranged by size: 4858 794 287 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 3263 3263 Size: 5959 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2667 3406 Size: 54 1425 50 275 18 426 411 739 Cuts at: 3406 4295 5924 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 739 426 411 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3889 1353 Size: 2536 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3022 3032 4546 4920 5066 387 Size: 1064 138 1433 10 1514 374 146 1280 Fragments arranged by size: 1514 1433 1280 1064 374 146 138 10 EcoICRI GAG'CTC Cuts at: 2539 2539 Size: 5959 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2419 4022 4619 5406 5674 1239 Size: 393 554 233 1603 597 787 268 1524 Fragments arranged by size: 1603 1524 787 597 554 393 268 233 EcoO109I rG'GnC_Cy Cuts at: 2020 2021 2029 2048 2049 2123 2317 2608 2020 Size: 1 8 19 1 74 194 291 5371 Fragments arranged by size: 5371 291 194 74 19 8 1 1 EcoRI G'AATT_C Cuts at: 2039 2926 3268 3646 2039 Size: 887 342 378 4352 Fragments arranged by size: 4352 887 378 342 EcoRV GAT'ATC Cuts at: 2752 2752 Size: 5959 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2061 2192 2231 2381 5106 1678 Size: 383 131 39 150 2725 2531 Fragments arranged by size: 2725 2531 383 150 131 39 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2523 2555 2580 2612 3054 Size: 32 135 32 951 32 25 32 442 Cuts at: 3054 3086 4600 4632 4674 4706 1373 Size: 32 1514 32 42 32 2626 Fragments arranged by size: 2626 1514 951 442 135 42 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3303 3549 669 Size: 988 281 1365 246 3079 Fragments arranged by size: 3079 1365 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2057 2057 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2669 10 Size: 54 1425 343 837 3300 Fragments arranged by size: 3300 1425 837 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2744 3917 4276 4435 518 Size: 648 1578 1173 359 159 2042 Fragments arranged by size: 2042 1578 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2063 2360 3375 4052 5200 5445 1606 Size: 429 28 297 1015 677 1148 245 2120 Fragments arranged by size: 2120 1148 1015 677 429 297 245 28 NaeI GCC'GGC Cuts at: 2244 3224 2244 Size: 980 4979 NarI GG'CG_CC Cuts at: 2058 2058 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2004 2745 2868 513 Size: 552 939 741 123 3604 Fragments arranged by size: 3604 939 741 552 123 NdeI CA'TA_TG Cuts at: 387 387 Size: 5959 NgoMIV G'CCGG_C Cuts at: 2242 3222 2242 Size: 980 4979 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 2053 2857 1348 Size: 705 804 4450 Fragments arranged by size: 4450 804 705 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 1108 Size: 1212 4747 PfoI T'CCnGG_A Cuts at: 2382 2382 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2608 2608 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 2283 1613 Size: 670 5289 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PspOMI G'GGCC_C Cuts at: 2020 2028 2048 2122 2020 Size: 8 20 74 5857 Fragments arranged by size: 5857 74 20 8 PstI C_TGCA'G Cuts at: 830 2223 2826 3192 3311 830 Size: 1393 603 366 119 3478 Fragments arranged by size: 3478 1393 603 366 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 SacI G_AGCT'C Cuts at: 2541 2541 Size: 5959 SacII CC_GC'GG Cuts at: 2064 2064 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 2223 2826 3311 2223 Size: 603 485 4871 Fragments arranged by size: 4871 603 485 ScaI AGT'ACT Cuts at: 3218 4207 5919 3218 Size: 989 1712 3258 Fragments arranged by size: 3258 1712 989 SfcI C'TryA_G Cuts at: 826 1045 2219 2815 2822 2994 3188 3307 4442 Size: 219 1174 596 7 172 194 119 1135 Cuts at: 4442 5123 5314 826 Size: 681 191 1471 Fragments arranged by size: 1471 1174 1135 681 596 219 194 191 172 119 7 SfoI GGC'GCC Cuts at: 2059 2059 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 2027 2161 3170 3286 1872 Size: 155 134 1009 116 4545 Fragments arranged by size: 4545 1009 155 134 116 SmlI C'TyrA_G Cuts at: 820 1017 1943 2981 4063 4964 5226 820 Size: 197 926 1038 1082 901 262 1553 Fragments arranged by size: 1553 1082 1038 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SrfI GCCC'GGGC Cuts at: 2161 2161 Size: 5959 SspI AAT'ATT Cuts at: 5 52 2573 3883 5 Size: 47 2521 1310 2081 Fragments arranged by size: 2521 2081 1310 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2745 2868 3398 513 Size: 552 939 396 345 123 530 3074 Fragments arranged by size: 3074 939 552 530 396 345 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 2791 3015 3986 4003 4156 4341 Size: 1255 1116 95 224 971 17 153 185 Cuts at: 4341 4786 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1116 971 445 224 185 153 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3216 4205 5917 Size: 274 80 33 51 1443 1239 989 1712 Cuts at: 5917 96 Size: 138 Fragments arranged by size: 1712 1443 1239 989 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2159 2191 5919 5951 2159 Size: 32 3728 32 2167 Fragments arranged by size: 3728 2167 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 2025 2159 3168 3284 1870 Size: 155 134 1009 116 4545 Fragments arranged by size: 4545 1009 155 134 116 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SapI SexAI SfiI SwaI XcmI