Restriction map for
pFN21AB3993
(Linear) (Six-base) MAPSORT of: KIBB3993.seq Check: 7187 from: 1 to: 1254
KIBB3993.orf
With 182 enzymes: SgfI *
November 16, 2017 14:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 104 1115 1254
Size: 104 1011 139
Fragments arranged by size:
1011 139 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 1254
Size: 414 840
AlwNI CAG_nnn'CTG
Cuts at: 0 315 831 852 1182 1254
Size: 315 516 21 330 72
Fragments arranged by size:
516 330 315 72 21
ApaI G_GGCC'C
Cuts at: 0 19 27 47 121 1254
Size: 19 8 20 74 1133
Fragments arranged by size:
1133 74 20 19 8
ApaLI G'TGCA_C
Cuts at: 0 1150 1254
Size: 1150 104
ApoI r'AATT_y
Cuts at: 0 34 921 1254
Size: 34 887 333
Fragments arranged by size:
887 333 34
AvaI C'yCGr_G
Cuts at: 0 20 154 1163 1254
Size: 20 134 1009 91
Fragments arranged by size:
1009 134 91 20
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 528 561 1254
Size: 528 33 693
Fragments arranged by size:
693 528 33
BanI G'GyrC_C
Cuts at: 0 52 480 798 1254
Size: 52 428 318 456
Fragments arranged by size:
456 428 318 52
BanII G_rGCy'C
Cuts at: 0 19 27 47 121 178 536 1254
Size: 19 8 20 74 57 358 718
Fragments arranged by size:
718 358 74 57 20 19 8
BbeI G_GCGC'C
Cuts at: 0 56 1254
Size: 56 1198
BglI GCCn_nnn'nGGC
Cuts at: 0 596 1254
Size: 596 658
Bme1580I G_kGCm'C
Cuts at: 0 19 27 47 121 803 1154 1254
Size: 19 8 20 74 682 351 100
Fragments arranged by size:
682 351 100 74 20 19 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 460 819 1254
Size: 460 359 435
Fragments arranged by size:
460 435 359
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1002 1254
Size: 1002 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1254
Size: 181 1073
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1254
Size: 997 257
BsaI GGTCTCn'nnnn_
Cuts at: 0 973 1052 1254
Size: 973 79 202
Fragments arranged by size:
973 202 79
BsaAI yAC'GTr
Cuts at: 0 699 1254
Size: 699 555
BsaHI Gr'CG_yC
Cuts at: 0 53 1254
Size: 53 1201
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 154 184 1254
Size: 154 30 1070
Fragments arranged by size:
1070 154 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 736 1117 1254
Size: 736 381 137
Fragments arranged by size:
736 381 137
BseYI C'CCAG_C
Cuts at: 0 132 342 599 970 1254
Size: 132 210 257 371 284
Fragments arranged by size:
371 284 257 210 132
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1254
Size: 820 434
BsiHKAI G_wGCw'C
Cuts at: 0 536 1154 1254
Size: 536 618 100
Fragments arranged by size:
618 536 100
Bsp1286I G_dGCh'C
Cuts at: 0 19 27 47 121 178 536 803 1154
Size: 19 8 20 74 57 358 267 351
Cuts at: 1154 1254
Size: 100
Fragments arranged by size:
358 351 267 100 74 57 20 19
8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 447 1115 1254
Size: 104 343 668 139
Fragments arranged by size:
668 343 139 104
BsrBI CCG'CTC
Cuts at: 0 1173 1254
Size: 1173 81
BsrFI r'CCGG_y
Cuts at: 0 237 1217 1254
Size: 237 980 37
Fragments arranged by size:
980 237 37
BssHII G'CGCG_C
Cuts at: 0 124 241 1254
Size: 124 117 1013
Fragments arranged by size:
1013 124 117
BssSI C'ACGA_G
Cuts at: 0 718 1254
Size: 718 536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1182 1254
Size: 1182 72
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 1254
Size: 672 582
BtgI C'CryG_G
Cuts at: 0 56 178 740 863 912 1254
Size: 56 122 562 123 49 342
Fragments arranged by size:
562 342 123 122 56 49
BtsI GCAGTG_nn'
Cuts at: 0 1183 1254
Size: 1183 71
EaeI y'GGCC_r
Cuts at: 0 251 662 1254
Size: 251 411 592
Fragments arranged by size:
592 411 251
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1017 1027 1254
Size: 1017 10 227
Fragments arranged by size:
1017 227 10
EcoICRI GAG'CTC
Cuts at: 0 534 1254
Size: 534 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 414 1254
Size: 181 233 840
Fragments arranged by size:
840 233 181
EcoO109I rG'GnC_Cy
Cuts at: 0 15 16 24 43 44 118 312 603
Size: 15 1 8 19 1 74 194 291
Cuts at: 603 1254
Size: 651
Fragments arranged by size:
651 291 194 74 19 15 8 1
1
EcoRI G'AATT_C
Cuts at: 0 34 921 1254
Size: 34 887 333
Fragments arranged by size:
887 333 34
EcoRV GAT'ATC
Cuts at: 0 747 1254
Size: 747 507
HaeII r_GCGC'y
Cuts at: 0 56 187 226 376 1254
Size: 56 131 39 150 878
Fragments arranged by size:
878 150 131 56 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 575 607 1049 1081 1254
Size: 518 32 25 32 442 32 173
Fragments arranged by size:
518 442 173 32 32 32 25
KasI G'GCGC_C
Cuts at: 0 52 1254
Size: 52 1202
MscI TGG'CCA
Cuts at: 0 664 1254
Size: 664 590
MslI CAynn'nnrTG
Cuts at: 0 739 1254
Size: 739 515
MspA1I CmG'CkG
Cuts at: 0 30 58 355 1254
Size: 30 28 297 899
Fragments arranged by size:
899 297 30 28
NaeI GCC'GGC
Cuts at: 0 239 1219 1254
Size: 239 980 35
Fragments arranged by size:
980 239 35
NarI GG'CG_CC
Cuts at: 0 53 1254
Size: 53 1201
NcoI C'CATG_G
Cuts at: 0 740 863 1254
Size: 740 123 391
Fragments arranged by size:
740 391 123
NgoMIV G'CCGG_C
Cuts at: 0 237 1217 1254
Size: 237 980 37
Fragments arranged by size:
980 237 37
PasI CC'CwG_GG
Cuts at: 0 48 852 1254
Size: 48 804 402
Fragments arranged by size:
804 402 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 315 1254
Size: 315 939
PfoI T'CCnGG_A
Cuts at: 0 377 1254
Size: 377 877
PpuMI rG'GwC_Cy
Cuts at: 0 603 1254
Size: 603 651
PshAI GACnn'nnGTC
Cuts at: 0 278 1254
Size: 278 976
PspOMI G'GGCC_C
Cuts at: 0 15 23 43 117 1254
Size: 15 8 20 74 1137
Fragments arranged by size:
1137 74 20 15 8
PstI C_TGCA'G
Cuts at: 0 218 821 1187 1254
Size: 218 603 366 67
Fragments arranged by size:
603 366 218 67
SacI G_AGCT'C
Cuts at: 0 536 1254
Size: 536 718
SacII CC_GC'GG
Cuts at: 0 59 1254
Size: 59 1195
SbfI CC_TGCA'GG
Cuts at: 0 218 821 1254
Size: 218 603 433
Fragments arranged by size:
603 433 218
ScaI AGT'ACT
Cuts at: 0 1213 1254
Size: 1213 41
SfcI C'TryA_G
Cuts at: 0 214 810 817 989 1183 1254
Size: 214 596 7 172 194 71
Fragments arranged by size:
596 214 194 172 71 7
SfoI GGC'GCC
Cuts at: 0 54 1254
Size: 54 1200
SmaI CCC'GGG
Cuts at: 0 22 156 1165 1254
Size: 22 134 1009 89
Fragments arranged by size:
1009 134 89 22
SmlI C'TyrA_G
Cuts at: 0 976 1254
Size: 976 278
SrfI GCCC'GGGC
Cuts at: 0 156 1254
Size: 156 1098
SspI AAT'ATT
Cuts at: 0 568 1254
Size: 568 686
StyI C'CwwG_G
Cuts at: 0 395 740 863 1254
Size: 395 345 123 391
Fragments arranged by size:
395 391 345 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 786 1010 1254
Size: 691 95 224 244
Fragments arranged by size:
691 244 224 95
TatI w'GTAC_w
Cuts at: 0 1211 1254
Size: 1211 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1254
Size: 154 32 1068
Fragments arranged by size:
1068 154 32
XmaI C'CCGG_G
Cuts at: 0 20 154 1163 1254
Size: 20 134 1009 91
Fragments arranged by size:
1009 134 91 20
Enzymes that do cut:
AarI AcuI AlwNI ApaI ApaLI ApoI AvaI BaeI
BanI BanII BbeI BglI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI
BstXI BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PasI PflMI PfoI PpuMI
PshAI PspOMI PstI SacI SacII SbfI ScaI SfcI
SfoI SmaI SmlI SrfI SspI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvrII
BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI Bpu10I BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EarI EcoNI FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SalI SanDI SapI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI StuI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI