Restriction map for
pFN21AE1733
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BclI BfrI BglI BglII
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
CelII Cfr10I ClaI DraI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 6 CGCG CG^CG
AfaI 269 GTAC GT^AC
AlwI 108 GGATC GGATC(4/5)
AlwNI 436 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 568 CCTCAGG CC^T[ACGT]AGG
AspI 172 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 509 CCCGGG C^[CT]CG[GA]G
AvrII 523 CCTAGG C^CTAGG
BcgI 613 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 166 GGATAC GTATCC(6/5)
BlnI 523 CCTAGG C^CTAGG
BpmI 358 CTGGAG CTGGAG(16/14)
BsgI 658 GTGCAG GTGCAG(16/14)
BsmAI 101 GTCTC GTCTC(1/5)
BsoBI 509 CCCGGG C^[CT]CG[GA]G
Bsp1407I 268 TGTACA T^GTACA
BsrDI 197 CATTGC GCAATG(2/0)
BsrGI 268 TGTACA T^GTACA
BstAPI 211 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 6 CGCG CG^CG
Bsu36I 568 CCTCAGG CC^T[ACGT]AGG
CfoI 7 GCGC GCG^C
CpoI 92 CGGACCG CG^G[AT]CCG
DpnII 109 GATC ^GATC
DraIII 295 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCG [CT]^GGCC[GA]
Eco81I 568 CCTCAGG CC^T[ACGT]AGG
EcoNI 354 CCTCCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 523 CCTAGG C^C[AT][AT]GG
FauI 64 GCGGG CCCGC(4/6)
HapII 510 CCGG C^CGG
HgaI 12 GCGTC GACGC(5/10)
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HpaII 510 CCGG C^CGG
HphI 686 TCACC GGTGA(8/7)
MaeII 49 ACGT A^CGT
MaeIII 652 GTGAC ^GT[ACGT]AC
MboI 109 GATC ^GATC
MboII 457 TCTTC GAAGA(8/7)
MspA1I 96 CCGCTG C[AC]G^C[GT]G
MspI 510 CCGG C^CGG
MvnI 6 CGCG CG^CG
NdeII 109 GATC ^GATC
NspI 117 GCATGC [GA]CATG^[CT]
PflFI 172 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 94 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 202 CTGCAG CTGCA^G
RsaI 269 GTAC GT^AC
RsrII 92 CGGACCG CG^G[AT]CCG
Sau3AI 109 GATC ^GATC
SfcI 202 CTGCAG C^T[GA][CT]AG
SmaI 509 CCCGGG CCC^GGG
SphI 117 GCATGC GCATG^C
SspBI 268 TGTACA T^GTACA
StuI 273 AGGCCT AGG^CCT
StyI 523 CCTAGG C^C[AT][AT]GG
Tsp45I 652 GTGAC ^GT[GC]AC
Tth111I 172 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 509 CCCGGG C^CCGGG
XmaI 509 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 13 enzymes) #
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AluI 536 AGCT AG^CT
665 AGCT
BanII 66 GGGCTC G[GA]GC[CT]^C
184 GGGCTC
BcnI 509 CCCGG CC^[GC]GG
510 CCGGG
BfaI 524 CTAG C^TAG
667 CTAG
BsmI 17 GAATGC GAATGC(1/-1)
547 GAATGC
HinfI 582 GATTC G^A[ACGT]TC
705 GATTC
MaeI 524 CTAG C^TAG
667 CTAG
MslI 380 CACCTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
424 CACGCCGATG
NciI 509 CCCGG CC^[GC]GG
510 CCGGG
SmlI 405 CTCAAG C^T[CT][GA]AG
552 CTTGAG
TaqI 15 TCGA T^CGA
152 TCGA
TfiI 582 GATTC G^A[AT]TC
705 GATTC
TthHB8I 15 TCGA T^CGA
152 TCGA
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# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
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AciI AvaII BbvI BmyI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII ItaI MnlI MvaI
MwoI NlaIII NlaIV PpuMI Sau96I
ScrFI SfaNI TseI TspRI