(Circular) (Six-base) MAPSORT of: pFN21AE1733.seq Check: 8764 from: 1 to: 5425 pFN21AE1733 5425 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3235 278 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2768 1937 Size: 831 4594 Acc65I G'GTAC_C Cuts at: 2746 2746 Size: 5425 AclI AA'CG_TT Cuts at: 1563 3552 3925 1563 Size: 1989 373 3063 Fragments arranged by size: 3063 1989 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2448 3488 4872 5140 1632 Size: 816 1040 1384 268 1917 Fragments arranged by size: 1917 1384 1040 816 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5228 AflIII A'CryG_T Cuts at: 1203 4324 5301 1203 Size: 3121 977 1327 Fragments arranged by size: 3121 1327 977 AgeI A'CCGG_T Cuts at: 4256 4256 Size: 5425 AhdI GACnn_n'nnGTC Cuts at: 4154 4154 Size: 5425 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5425 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2451 1184 Size: 1267 4158 AlwNI CAG_nnn'CTG Cuts at: 2446 3168 4740 2446 Size: 722 1572 3131 Fragments arranged by size: 3131 1572 722 ApaLI G'TGCA_C Cuts at: 3482 4638 3482 Size: 1156 4269 ApoI r'AATT_y Cuts at: 1493 2734 2960 3112 5221 1493 Size: 1241 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 226 152 AseI AT'TA_AT Cuts at: 160 1032 3979 160 Size: 872 2947 1606 Fragments arranged by size: 2947 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 AvaI C'yCGr_G Cuts at: 1870 1943 2514 2750 1870 Size: 73 571 236 4545 Fragments arranged by size: 4545 571 236 73 AvrII C'CTAG_G Cuts at: 2528 2528 Size: 5425 BamHI G'GATC_C Cuts at: 2755 3122 2755 Size: 367 5058 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2746 4201 618 Size: 325 217 627 959 1455 1842 Fragments arranged by size: 1842 1455 959 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 2193 2744 1391 Size: 684 118 551 4072 Fragments arranged by size: 4072 684 551 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4454 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2607 2641 3616 3650 1903 Size: 34 670 34 975 34 3678 Fragments arranged by size: 3678 975 670 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2165 3317 4527 2165 Size: 1152 1210 3063 Fragments arranged by size: 3063 1210 1152 BclI T'GATC_A Cuts at: 1548 1548 Size: 5425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4036 136 Size: 107 122 71 631 605 2364 1525 Fragments arranged by size: 2364 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5420 5420 Size: 5425 BlpI GC'TnA_GC Cuts at: 2842 2842 Size: 5425 Bme1580I G_kGCm'C Cuts at: 1790 2144 3486 4642 1790 Size: 354 1342 1156 2573 Fragments arranged by size: 2573 1342 1156 354 BmgBI CAC'GTC Cuts at: 1318 2055 1318 Size: 737 4688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2297 3156 4114 Size: 416 53 83 399 24 880 859 958 Cuts at: 4114 442 Size: 1753 Fragments arranged by size: 1753 958 880 859 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5425 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 4085 1239 Size: 1145 1701 2579 Fragments arranged by size: 2579 1701 1145 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2395 2578 3550 4415 4713 2395 Size: 183 972 865 298 3107 Fragments arranged by size: 3107 972 865 298 183 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4088 882 Size: 617 2589 2219 Fragments arranged by size: 2589 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3216 493 Size: 711 2012 2702 Fragments arranged by size: 2702 2012 711 BsaBI GATnn'nnATC Cuts at: 5419 5419 Size: 5425 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3232 3614 4249 275 Size: 53 83 186 936 1699 382 635 1451 Fragments arranged by size: 1699 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3858 4256 4530 4677 5088 3858 Size: 398 274 147 411 4195 Fragments arranged by size: 4195 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2278 2308 1178 Size: 30 165 30 875 30 4295 Fragments arranged by size: 4295 875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5425 BseYI C'CCAG_C Cuts at: 2127 2314 4628 5124 2127 Size: 187 2314 496 2428 Fragments arranged by size: 2428 2314 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2684 1163 Size: 573 948 3904 Fragments arranged by size: 3904 948 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2875 3636 4664 1540 Size: 275 186 874 761 1028 2301 Fragments arranged by size: 2301 1028 874 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3486 3571 4642 3486 Size: 85 1071 4269 Fragments arranged by size: 4269 1071 85 BsmI GAATG_Cn' Cuts at: 2028 2558 2936 3029 2028 Size: 530 378 93 4424 Fragments arranged by size: 4424 530 378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2144 2193 2744 3486 3571 4642 Size: 399 285 69 49 551 742 85 1071 Cuts at: 4642 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 551 399 285 85 69 49 BspEI T'CCGG_A Cuts at: 5088 5088 Size: 5425 BspHI T'CATG_A Cuts at: 3314 5044 3314 Size: 1730 3695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2780 844 Size: 1936 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3312 1394 Size: 429 1489 3507 Fragments arranged by size: 3507 1489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2201 3920 4094 65 Size: 1183 953 1719 174 1396 Fragments arranged by size: 1719 1396 1183 953 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4069 4256 1700 Size: 110 2259 187 2869 Fragments arranged by size: 2869 2259 187 110 BsrGI T'GTAC_A Cuts at: 96 2273 96 Size: 2177 3248 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5425 BssSI C'ACGA_G Cuts at: 3485 4497 3485 Size: 1012 4413 BstAPI GCAn_nnn'nTGC Cuts at: 794 2222 794 Size: 1428 3997 BstBI TT'CG_AA Cuts at: 4229 4229 Size: 5425 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5425 BstYI r'GATC_y Cuts at: 1972 2755 3122 3507 3524 4976 5062 5074 5420 Size: 783 367 385 17 1452 86 12 346 Cuts at: 5420 1972 Size: 1977 Fragments arranged by size: 1977 1452 783 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2574 2574 Size: 5425 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3934 Fragments arranged by size: 3934 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4686 Fragments arranged by size: 4686 683 56 BtsI GCAGTG_nn' Cuts at: 2941 3735 3755 2941 Size: 794 20 4611 Fragments arranged by size: 4611 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5425 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5390 DraI TTT'AAA Cuts at: 2729 2729 Size: 5425 DraIII CAC_nnn'GTG Cuts at: 2305 2305 Size: 5425 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4432 809 Size: 507 3116 1802 Fragments arranged by size: 3116 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2872 3761 Size: 54 1425 50 275 18 177 865 889 Cuts at: 3761 5390 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 865 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2872 1537 Size: 275 1060 4090 Fragments arranged by size: 4090 1060 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3355 1353 Size: 2002 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2111 4012 4386 4532 387 Size: 1064 138 522 1901 374 146 1280 Fragments arranged by size: 1901 1280 1064 522 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2448 3488 4085 4872 5140 1239 Size: 393 752 64 1040 597 787 268 1524 Fragments arranged by size: 1524 1040 787 752 597 393 268 64 EcoNI CCTnn'n_nnAGG Cuts at: 2363 2363 Size: 5425 EcoO109I rG'GnC_Cy Cuts at: 2580 2605 2640 2580 Size: 25 35 5365 Fragments arranged by size: 5365 35 25 EcoRI G'AATT_C Cuts at: 2734 3112 2734 Size: 378 5047 FspI TGC'GCA Cuts at: 3931 3931 Size: 5425 HaeII r_GCGC'y Cuts at: 1678 4572 1678 Size: 2894 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4066 4098 4140 4172 1373 Size: 32 135 32 2494 32 42 32 2626 Fragments arranged by size: 2626 2494 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2769 3015 669 Size: 988 281 831 246 3079 Fragments arranged by size: 3079 988 831 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5425 HpaI GTT'AAC Cuts at: 3015 3015 Size: 5425 KpnI G_GTAC'C Cuts at: 2750 2750 Size: 5425 MfeI C'AATT_G Cuts at: 3024 3024 Size: 5425 MluI A'CGCG_T Cuts at: 5301 5301 Size: 5425 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2500 4539 4723 1305 Size: 1195 2039 184 2007 Fragments arranged by size: 2039 2007 1195 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3603 Fragments arranged by size: 3603 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2389 2433 3383 3742 3901 518 Size: 648 1223 44 950 359 159 2042 Fragments arranged by size: 2042 1223 950 648 359 159 44 MspA1I CmG'CkG Cuts at: 1606 2103 2841 3518 4666 4911 1606 Size: 497 738 677 1148 245 2120 Fragments arranged by size: 2120 1148 738 677 497 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3934 Fragments arranged by size: 3934 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5425 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5425 NotI GC'GGCC_GC Cuts at: 2872 2872 Size: 5425 NspI r_CATG'y Cuts at: 1132 1229 2126 2783 4328 1132 Size: 97 897 657 1545 2229 Fragments arranged by size: 2229 1545 897 657 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5425 PciI A'CATG_T Cuts at: 4324 4324 Size: 5425 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5425 PfoI T'CCnGG_A Cuts at: 2361 2361 Size: 5425 PmeI GTTT'AAAC Cuts at: 2729 2729 Size: 5425 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3494 3526 5007 5039 3494 Size: 32 1481 32 3880 Fragments arranged by size: 3880 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2580 2605 2640 2580 Size: 25 35 5365 Fragments arranged by size: 5365 35 25 PshAI GACnn'nnGTC Cuts at: 1613 2103 1613 Size: 490 4935 PsiI TTA'TAA Cuts at: 2995 2995 Size: 5425 PstI C_TGCA'G Cuts at: 830 2211 2777 830 Size: 1381 566 3478 Fragments arranged by size: 3478 1381 566 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5425 RsrII CG'GwC_CG Cuts at: 2098 2098 Size: 5425 SalI G'TCGA_C Cuts at: 1936 2767 1936 Size: 831 4594 SanDI GG'GwC_CC Cuts at: 2580 2580 Size: 5425 SbfI CC_TGCA'GG Cuts at: 2777 2777 Size: 5425 ScaI AGT'ACT Cuts at: 3673 5385 3673 Size: 1712 3713 SfcI C'TryA_G Cuts at: 826 1045 2207 2773 3908 4589 4780 826 Size: 219 1162 566 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 681 566 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5425 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5425 SmaI CCC'GGG Cuts at: 1872 2516 2752 1872 Size: 644 236 4545 Fragments arranged by size: 4545 644 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 2410 2557 3529 4430 4692 820 Size: 197 926 467 147 972 901 262 1553 Fragments arranged by size: 1553 972 926 901 467 262 197 147 SnaBI TAC'GTA Cuts at: 493 3216 493 Size: 2723 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5425 SphI G_CATG'C Cuts at: 1132 2126 2783 1132 Size: 994 657 3774 Fragments arranged by size: 3774 994 657 SspI AAT'ATT Cuts at: 5 52 3349 5 Size: 47 3297 2081 Fragments arranged by size: 3297 2081 47 StuI AGG'CCT Cuts at: 1517 2280 1517 Size: 763 4662 StyI C'CwwG_G Cuts at: 513 1065 2004 2528 2864 513 Size: 552 939 524 336 3074 Fragments arranged by size: 3074 939 552 524 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 3452 3469 3622 3807 4252 325 Size: 1255 736 1136 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1136 736 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2273 3671 5383 Size: 274 80 33 51 1443 296 1398 1712 Cuts at: 5383 96 Size: 138 Fragments arranged by size: 1712 1443 1398 296 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2278 2310 5385 5417 2278 Size: 32 3075 32 2286 Fragments arranged by size: 3075 2286 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2180 1316 Size: 864 4561 XbaI T'CTAG_A Cuts at: 2761 2761 Size: 5425 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5425 XmaI C'CCGG_G Cuts at: 1870 2514 2750 1870 Size: 644 236 4545 Fragments arranged by size: 4545 644 236 XmnI GAAnn'nnTTC Cuts at: 3554 3554 Size: 5425 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3233 276 Size: 53 83 186 936 1699 2468 Fragments arranged by size: 2468 1699 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI