Restriction map for
pFN21AE5242
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp700I AspI AvaI AvaII AviII
AvrII BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FspI Hin1I
HincII HindII HindIII HpaI KasI
MaeIII MamI MfeI MluI MroI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 110 GGTACC G^GTACC
AccI 298 GTAGAC GT^[AC][GT]AC
AhdI 266 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 448 GTGCAC G^TGCAC
AlwNI 364 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 448 GTGCAC G^TGCAC
Asp718I 110 GGTACC G^GTACC
AspEI 266 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 519 TGGCCA TGG^CCA
BamHI 311 GGATCC G^GATCC
BclI 203 TGATCA T^GATCA
BfaI 268 CTAG C^TAG
BglI 25 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 288 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 288 GCTCAGC GC^T[ACGT]AGC
BsaAI 385 TACGTG [CT]AC^GT[GA]
BsaWI 494 TCCGGT [AT]^CCGG[AT]
BsgI 560 CTGCAC GTGCAG(16/14)
BsmBI 226 GAGACG CGTCTC(1/5)
BsrFI 25 GCCGGC [GA]^CCGG[CT]
BssHII 143 GCGCGC G^CGCGC
BstXI 419 CCACTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 288 GCTCAGC GC^T[ACGT]AGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
Cfr13I 372 GGGCC G^G[ACGT]CC
DdeI 289 CTCAG C^T[ACGT]AG
DsaI 75 CCGCGG C^C[GA][CT]GG
Eam1105I 266 GACTAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 320 CCTTGG C^C[AT][AT]GG
FbaI 203 TGATCA T^GATCA
FseI 24 GGCCGGCC GGCCGG^CC
HaeII 404 GGCGCT [GA]GCGC^[CT]
KpnI 110 GGTACC GGTAC^C
KspI 75 CCGCGG CCGC^GG
MaeI 268 CTAG C^TAG
MluNI 519 TGGCCA TGG^CCA
MscI 519 TGGCCA TGG^CCA
MseI 536 TTAA T^TAA
NaeI 25 GCCGGC GCC^GGC
NgoMIV 25 GCCGGC G^CCGGC
PflMI 363 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 582 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 75 CCGCGG CCGC^GG
SapI 42 GCTCTTC GCTCTTC(1/4)
Sau96I 372 GGGCC G^G[ACGT]CC
SfaNI 119 GCATC GCATC(5/9)
SmlI 355 CTCAAG C^T[CT][GA]AG
StuI 514 AGGCCT AGG^CCT
StyI 320 CCTTGG C^C[AT][AT]GG
TfiI 441 GATTC G^A[AT]TC
Tru9I 536 TTAA T^TAA
Tsp509I 347 AATT ^AATT
TspRI 376 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 363 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 40 GTGCTC G[AT]GC[AT]^C
448 GTGCAC
BanI 84 GGTGCC G^G[CT][GA]CC
110 GGTACC
BcnI 88 CCGGG CC^[GC]GG
102 CCGGG
BmyI 40 GTGCTC G[AGT]GC[ACT]^C
448 GTGCAC
BsaJI 75 CCGCGG C^C[ACGT][ACGT]GG
320 CCTTGG
BseRI 217 GAGGAG GAGGAG(10/8)
466 GAGGAG
BsiHKAI 40 GTGCTC G[AT]GC[AT]^C
448 GTGCAC
BsmAI 226 GAGAC GTCTC(1/5)
264 GAGAC
BsmFI 104 GGGAC GGGAC(10/14)
580 GGGAC
Bsp1286I 40 GTGCTC G[AGT]GC[ACT]^C
448 GTGCAC
BstYI 311 GGATCC [GA]^GATC[CT]
491 AGATCC
EaeI 5 CGGCCA [CT]^GGCC[GA]
519 TGGCCA
EcoNI 330 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
438 CCTGATTCAGG
HgaI 54 GACGC GACGC(5/10)
165 GACGC
HphI 201 GGTGA GGTGA(8/7)
206 TCACC
MaeII 191 ACGT A^CGT
386 ACGT
MflI 311 GGATCC [GA]^GATC[CT]
491 AGATCC
MslI 251 CACAGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
420 CACTATGGTG
NciI 88 CCGGG CC^[GC]GG
102 CCGGG
NlaIII 210 CATG CATG^
303 CATG
TaqI 122 TCGA T^CGA
275 TCGA
TthHB8I 122 TCGA T^CGA
275 TCGA
XhoII 311 GGATCC [GA]^GATC[CT]
491 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BpmI BsiYI BslI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIV
PleI PvuII RsaI Sau3AI ScrFI
TseI XcmI