Restriction map for
pFN21AE5242
(Circular) (Six-base) MAPSORT of: pFN21AE5242.seq Check: 6174 from: 1 to: 5308
pFN21AE5242 5308 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3118 278
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2304 2651 1937
Size: 367 347 4594
Fragments arranged by size:
4594 367 347
Acc65I G'GTAC_C
Cuts at: 2115 2629 2115
Size: 514 4794
AclI AA'CG_TT
Cuts at: 1563 3435 3808 1563
Size: 1872 373 3063
Fragments arranged by size:
3063 1872 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3371 4755 5023 1632
Size: 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5111
AflIII A'CryG_T
Cuts at: 1203 4207 5184 1203
Size: 3004 977 1327
Fragments arranged by size:
3004 1327 977
AgeI A'CCGG_T
Cuts at: 4139 4139
Size: 5308
AhdI GACnn_n'nnGTC
Cuts at: 2276 4037 2276
Size: 1761 3547
AleI CACnn'nnGTG
Cuts at: 1166 2260 2429 1166
Size: 1094 169 4045
Fragments arranged by size:
4045 1094 169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2580 1184
Size: 1396 3912
AlwNI CAG_nnn'CTG
Cuts at: 2374 3051 4623 2374
Size: 677 1572 3059
Fragments arranged by size:
3059 1572 677
ApaLI G'TGCA_C
Cuts at: 2453 3365 4521 2453
Size: 912 1156 3240
Fragments arranged by size:
3240 1156 912
ApoI r'AATT_y
Cuts at: 1493 2617 2843 2995 5104 1493
Size: 1124 226 152 2109 1697
Fragments arranged by size:
2109 1697 1124 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3862 160
Size: 872 2830 1606
Fragments arranged by size:
2830 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
AvaI C'yCGr_G
Cuts at: 1870 1943 2633 1870
Size: 73 690 4545
Fragments arranged by size:
4545 690 73
BamHI G'GATC_C
Cuts at: 2316 2638 3005 2316
Size: 322 367 4619
Fragments arranged by size:
4619 367 322
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2089 2115 2629 4084 618
Size: 325 217 627 302 26 514 1455 1842
Fragments arranged by size:
1842 1455 627 514 325 302 217 26
BanII G_rGCy'C
Cuts at: 1391 2627 1391
Size: 1236 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3499 3533 1903
Size: 34 1562 34 3678
Fragments arranged by size:
3678 1562 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3200 4410 3200
Size: 1210 4098
BclI T'GATC_A
Cuts at: 1548 2208 1548
Size: 660 4648
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2036 3919
Size: 107 122 71 631 605 337 27 1883
Cuts at: 3919 136
Size: 1525
Fragments arranged by size:
1883 1525 631 605 337 122 107 71
27
BglII A'GATC_T
Cuts at: 5303 5303
Size: 5308
BlpI GC'TnA_GC
Cuts at: 2294 2725 2294
Size: 431 4877
Bme1580I G_kGCm'C
Cuts at: 1790 2457 3369 4525 1790
Size: 667 912 1156 2573
Fragments arranged by size:
2573 1156 912 667
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5308
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2322 3039 3997
Size: 416 53 83 399 24 905 717 958
Cuts at: 3997 442
Size: 1753
Fragments arranged by size:
1753 958 905 717 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 2321 2398 3968 1239
Size: 806 276 77 1570 2579
Fragments arranged by size:
2579 1570 806 276 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2345 3433 4298 4596 2345
Size: 1088 865 298 3057
Fragments arranged by size:
3057 1088 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3971 882
Size: 617 2472 2219
Fragments arranged by size:
2472 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 3099 493
Size: 711 1188 707 2702
Fragments arranged by size:
2702 1188 711 707
BsaBI GATnn'nnATC
Cuts at: 5302 5302
Size: 5308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3115 3497 4132 275
Size: 53 83 186 936 1582 382 635 1451
Fragments arranged by size:
1582 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2499 3741 4139 4413 4560 4971 2499
Size: 1242 398 274 147 411 2836
Fragments arranged by size:
2836 1242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5083
Fragments arranged by size:
5083 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2237 2486 1186
Size: 1051 249 4008
Fragments arranged by size:
4008 1051 249
BseYI C'CCAG_C
Cuts at: 2143 2374 2405 4511 5007 2143
Size: 231 31 2106 496 2444
Fragments arranged by size:
2444 2106 496 231 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2550 1163
Size: 573 814 3921
Fragments arranged by size:
3921 814 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2758 3519 4547 1540
Size: 275 186 757 761 1028 2301
Fragments arranged by size:
2301 1028 761 757 275 186
BsiHKAI G_wGCw'C
Cuts at: 2049 2457 3369 3454 4525 2049
Size: 408 912 85 1071 2832
Fragments arranged by size:
2832 1071 912 408 85
BsmI GAATG_Cn'
Cuts at: 2819 2912 2819
Size: 93 5215
BsmBI CGTCTCn'nnnn_
Cuts at: 2225 2225
Size: 5308
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2457 2627 3369 3454 4525 1391
Size: 399 259 408 170 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 408 399 259 170 85
BspEI T'CCGG_A
Cuts at: 4971 4971
Size: 5308
BspHI T'CATG_A
Cuts at: 3197 4927 3197
Size: 1730 3578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2663 844
Size: 1819 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3195 1394
Size: 429 1372 3507
Fragments arranged by size:
3507 1372 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3803 3977 65
Size: 1183 2555 174 1396
Fragments arranged by size:
2555 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 3952 4139 1700
Size: 110 220 1922 187 2869
Fragments arranged by size:
2869 1922 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5308
BssHII G'CGCG_C
Cuts at: 1925 2148 1925
Size: 223 5085
BssSI C'ACGA_G
Cuts at: 3368 4380 3368
Size: 1012 4296
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5308
BstBI TT'CG_AA
Cuts at: 4112 4112
Size: 5308
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5308
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2431
Size: 5308
BstYI r'GATC_y
Cuts at: 1972 2316 2496 2638 3005 3390 3407 4859 4945
Size: 344 180 142 367 385 17 1452 86
Cuts at: 4945 4957 5303 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 344 180 142
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2080 513
Size: 552 939 76 3741
Fragments arranged by size:
3741 939 552 76
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 487
Size: 683 56 1247 3322
Fragments arranged by size:
3322 1247 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3618 3638 2824
Size: 794 20 4494
Fragments arranged by size:
4494 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5308
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5273
DraI TTT'AAA
Cuts at: 2612 2612
Size: 5308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4315 809
Size: 507 2999 1802
Fragments arranged by size:
2999 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2524 2755
Size: 54 1425 50 275 18 180 514 231
Cuts at: 2755 3644 5273 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 514 275 231 180 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2755 1537
Size: 275 943 4090
Fragments arranged by size:
4090 943 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2257 2261 3238 1353
Size: 701 203 4 977 3423
Fragments arranged by size:
3423 977 701 203 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3895 4269 4415 387
Size: 1064 138 2306 374 146 1280
Fragments arranged by size:
2306 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2321 2398 3371 3968 4755 5023
Size: 393 413 276 77 973 597 787 268
Cuts at: 5023 1239
Size: 1524
Fragments arranged by size:
1524 973 787 597 413 393 276 268
77
EcoNI CCTnn'n_nnAGG
Cuts at: 2339 2447 2339
Size: 108 5200
EcoRI G'AATT_C
Cuts at: 2617 2995 2617
Size: 378 4930
FseI GG_CCGG'CC
Cuts at: 2034 2034
Size: 5308
FspI TGC'GCA
Cuts at: 3814 3814
Size: 5308
HaeII r_GCGC'y
Cuts at: 1678 2413 4455 1678
Size: 735 2042 2531
Fragments arranged by size:
2531 2042 735
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2262 2294 3949 3981 4023
Size: 32 135 32 690 32 1655 32 42
Cuts at: 4023 4055 1373
Size: 32 2626
Fragments arranged by size:
2626 1655 690 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2652 2898 669
Size: 988 281 714 246 3079
Fragments arranged by size:
3079 988 714 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5308
HpaI GTT'AAC
Cuts at: 2898 2898
Size: 5308
KpnI G_GTAC'C
Cuts at: 2119 2633 2119
Size: 514 4794
MfeI C'AATT_G
Cuts at: 2907 2907
Size: 5308
MluI A'CGCG_T
Cuts at: 5184 5184
Size: 5308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 4606 1305
Size: 3117 184 2007
Fragments arranged by size:
3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2526 10
Size: 54 1425 343 694 2792
Fragments arranged by size:
2792 1425 694 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2260 2429 3266 3625 3784 518
Size: 648 1094 169 837 359 159 2042
Fragments arranged by size:
2042 1094 837 648 359 169 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2179 2287 2530 2724 3401 4549 4794
Size: 476 97 108 243 194 677 1148 245
Cuts at: 4794 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 476 245 243 194 108
97
NaeI GCC'GGC
Cuts at: 2032 2032
Size: 5308
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3817
Fragments arranged by size:
3817 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5308
NgoMIV G'CCGG_C
Cuts at: 2030 2030
Size: 5308
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5308
NotI GC'GGCC_GC
Cuts at: 2755 2755
Size: 5308
NspI r_CATG'y
Cuts at: 1132 1229 2666 4211 1132
Size: 97 1437 1545 2229
Fragments arranged by size:
2229 1545 1437 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5308
PciI A'CATG_T
Cuts at: 4207 4207
Size: 5308
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2374 1108
Size: 1266 4042
PfoI T'CCnGG_A
Cuts at: 2414 2414
Size: 5308
PmeI GTTT'AAAC
Cuts at: 2612 2612
Size: 5308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2222 2254 3377 3409 4890 4922 2222
Size: 32 1123 32 1481 32 2608
Fragments arranged by size:
2608 1481 1123 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2591 1613
Size: 978 4330
PsiI TTA'TAA
Cuts at: 2878 2878
Size: 5308
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2210 2242 2210
Size: 32 5276
PstI C_TGCA'G
Cuts at: 830 2660 830
Size: 1830 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5308
PvuII CAG'CTG
Cuts at: 2179 2287 2530 2179
Size: 108 243 4957
Fragments arranged by size:
4957 243 108
SacII CC_GC'GG
Cuts at: 2083 2083
Size: 5308
SalI G'TCGA_C
Cuts at: 1936 2650 1936
Size: 714 4594
SapI GCTCTTCn'nnn_
Cuts at: 2054 2054
Size: 5308
SbfI CC_TGCA'GG
Cuts at: 2660 2660
Size: 5308
ScaI AGT'ACT
Cuts at: 3556 5268 3556
Size: 1712 3596
SfcI C'TryA_G
Cuts at: 826 1045 2656 3791 4472 4663 826
Size: 219 1611 1135 681 191 1471
Fragments arranged by size:
1611 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5308
SmaI CCC'GGG
Cuts at: 1872 2635 1872
Size: 763 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2360 3412 4313 4575 820
Size: 197 926 417 1052 901 262 1553
Fragments arranged by size:
1553 1052 926 901 417 262 197
SnaBI TAC'GTA
Cuts at: 493 3099 493
Size: 2606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5308
SphI G_CATG'C
Cuts at: 1132 2666 1132
Size: 1534 3774
SspI AAT'ATT
Cuts at: 5 52 3232 5
Size: 47 3180 2081
Fragments arranged by size:
3180 2081 47
StuI AGG'CCT
Cuts at: 1517 2521 1517
Size: 1004 4304
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2747 513
Size: 552 939 321 422 3074
Fragments arranged by size:
3074 939 552 422 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3335 3352 3505 3690 4135 325
Size: 1255 1755 17 153 185 445 1498
Fragments arranged by size:
1755 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3554 5266 96
Size: 274 80 33 51 1443 1577 1712 138
Fragments arranged by size:
1712 1577 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5268 5300 5268
Size: 32 5276
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5308
XbaI T'CTAG_A
Cuts at: 2644 2644
Size: 5308
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2140 2519 2020
Size: 120 379 4809
Fragments arranged by size:
4809 379 120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5308
XmaI C'CCGG_G
Cuts at: 1870 2633 1870
Size: 763 4545
XmnI GAAnn'nnTTC
Cuts at: 3437 3437
Size: 5308
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3116 276
Size: 53 83 186 936 1582 2468
Fragments arranged by size:
2468 1582 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FspAI KasI NarI NruI
NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacI
SanDI SexAI SfiI SfoI SrfI SwaI