################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI FseI FspI HpaI KasI KpnI KspI MamI MluI MroI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 57 CGCG CG^CG AciI 56 CCGC CCGC(-3/-1) AcyI 626 GGCGTC G[GA]^CG[CT]C AlwNI 206 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 300 AGCGCT AGC^GCT AseI 595 ATTAAT AT^TAAT AsnI 595 ATTAAT AT^TAAT AvaI 272 CTCGAG C^[CT]CG[GA]G AvaII 516 GGACC G^G[AT]CC BanI 167 GGCACC G^G[CT][GA]CC BclI 1365 TGATCA T^GATCA BcnI 70 CCGGG CC^[GC]GG BfaI 1480 CTAG C^TAG BglII 1215 AGATCT A^GATCT BsaAI 105 CACGTA [CT]AC^GT[GA] BsaHI 626 GGCGTC G[GA]^CG[CT]C BsaJI 722 CCATGG C^C[ACGT][ACGT]GG BsgI 10 GTGCAG GTGCAG(16/14) BsoBI 272 CTCGAG C^[CT]CG[GA]G BspMI 456 ACCTGC ACCTGC(4/8) BsrI 506 CCAGT ACTGG(1/-1) BstUI 57 CGCG CG^CG Cac8I 543 GCCAGC GC[ACGT]^[ACGT]GC Cfr13I 516 GGACC G^G[ACGT]CC DraII 515 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 722 CCATGG C^C[GA][CT]GG Eco47III 300 AGCGCT AGC^GCT EcoO109I 515 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 493 GATATC GAT^ATC EcoT14I 722 CCATGG C^C[AT][AT]GG EcoT22I 473 ATGCAT ATGCA^T FauI 55 CCCGC CCCGC(4/6) FbaI 1365 TGATCA T^GATCA HaeII 300 AGCGCT [GA]GCGC^[CT] HapII 70 CCGG C^CGG HgaI 627 GCGTC GACGC(5/10) Hin1I 626 GGCGTC G[GA]^CG[CT]C HpaII 70 CCGG C^CGG MaeI 1480 CTAG C^TAG MspI 70 CCGG C^CGG MvnI 57 CGCG CG^CG NciI 70 CCGGG CC^[GC]GG NcoI 722 CCATGG C^CATGG NsiI 473 ATGCAT ATGCA^T PaeR7I 272 CTCGAG C^TCGAG PflMI 834 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 515 AGGACCT [GA]G^G[AT]CC[CT] PshBI 595 ATTAAT AT^TAAT Sau96I 516 GGACC G^G[ACGT]CC StuI 64 AGGCCT AGG^CCT StyI 722 CCATGG C^C[AT][AT]GG TspRI 78 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 834 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 272 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 629 GTCTAC GT^[AC][GT]AC 643 GTAGAC AfaI 688 GTAC GT^AC 1225 GTAC Asp700I 325 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 820 GAATACCTTC BalI 2 TGGCCA TGG^CCA 1304 TGGCCA BsiYI 56 CCGCGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCAAATGGTGG BslI 56 CCGCGCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 834 CCAAATGGTGG BsmAI 1529 GAGAC GTCTC(1/5) 582 GTCTC BstF5I 488 CATCC GGATG(2/0) 216 GGATG BstNI 461 CCAGG CC^[AT]GG 1267 CCTGG BstYI 1215 AGATCT [GA]^GATC[CT] 1323 AGATCC EaeI 2 TGGCCA [CT]^GGCC[GA] 1304 TGGCCA EarI 134 CTCTTC CTCTTC(1/4) 1319 GAAGAG EcoRII 461 CCAGG ^CC[AT]GG 1267 CCTGG FokI 488 CATCC GGATG(9/13) 216 GGATG HincII 727 GTCAAC GT[CT]^[GA]AC 1183 GTTGAC HindII 727 GTCAAC GT[CT]^[GA]AC 1183 GTTGAC Ksp632I 134 CTCTTC CTCTTC(1/4) 1319 GAAGAG MfeI 620 CAATTG C^AATTG 806 CAATTG MflI 1215 AGATCT [GA]^GATC[CT] 1323 AGATCC MluNI 2 TGGCCA TGG^CCA 1304 TGGCCA MscI 2 TGGCCA TGG^CCA 1304 TGGCCA MunI 620 CAATTG C^AATTG 806 CAATTG MvaI 461 CCAGG CC^[AT]GG 1267 CCTGG PleI 661 GACTC GAGTC(4/5) 775 GACTC RsaI 688 GTAC GT^AC 1225 GTAC SmlI 272 CTCGAG C^T[CT][GA]AG 760 CTCAAG TaqI 273 TCGA T^CGA 750 TCGA TthHB8I 273 TCGA T^CGA 750 TCGA XhoII 1215 AGATCT [GA]^GATC[CT] 1323 AGATCC XmnI 325 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 820 GAATACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AluI AlwI ApoI BbsI BbvI BpuAI BssKI CfoI DdeI DpnII DraI Fnu4HI HaeIII HhaI HinP1I HindIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I