(Circular) (Six-base) MAPSORT of: pFN21AE3956.seq Check: 4320 from: 1 to: 6238 pFN21AE3956 6238 bp With 182 enzymes: SgfI * March 28, 2012 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2470 2470 Size: 6238 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2635 2649 3581 1937 Size: 698 14 932 4594 Fragments arranged by size: 4594 932 698 14 Acc65I G'GTAC_C Cuts at: 3559 3559 Size: 6238 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4301 5685 5953 1632 Size: 2669 1384 268 1917 Fragments arranged by size: 2669 1917 1384 268 AfeI AGC'GCT Cuts at: 2307 2307 Size: 6238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 5137 6114 1203 Size: 3934 977 1327 Fragments arranged by size: 3934 1327 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 2216 3981 5553 2216 Size: 1765 1572 2901 Fragments arranged by size: 2901 1765 1572 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2700 3074 3199 3424 3547 3773 3925 6034 Size: 1207 374 125 225 123 226 152 2109 Cuts at: 6034 1493 Size: 1697 Fragments arranged by size: 2109 1697 1207 374 226 225 152 125 123 AseI AT'TA_AT Cuts at: 160 1032 2601 4792 160 Size: 872 1569 2191 1606 Fragments arranged by size: 2191 1606 1569 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 2277 3563 1870 Size: 73 334 1286 4545 Fragments arranged by size: 4545 1286 334 73 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2172 3559 5014 618 Size: 325 217 627 385 1387 1455 1842 Fragments arranged by size: 1842 1455 1387 627 385 325 217 BanII G_rGCy'C Cuts at: 1391 3557 1391 Size: 2166 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3014 3088 3407 928 Size: 971 1115 74 319 3759 Fragments arranged by size: 3759 1115 971 319 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 3370 1548 Size: 1822 4416 BfrBI ATG'CAT Cuts at: 2480 2480 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3220 6233 3220 Size: 3013 3225 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3969 4927 442 Size: 416 53 83 399 24 2552 958 1753 Fragments arranged by size: 2552 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2780 2818 2780 Size: 38 6200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4898 1239 Size: 3659 2579 Bpu10I CC'TnA_GC Cuts at: 2871 2871 Size: 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2750 4363 5228 5526 2750 Size: 1613 865 298 3462 Fragments arranged by size: 3462 1613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4901 882 Size: 617 3402 2219 Fragments arranged by size: 3402 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2112 4029 493 Size: 711 908 1917 2702 Fragments arranged by size: 2702 1917 908 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2632 4045 4427 5062 Size: 53 83 186 936 1099 1413 382 635 Cuts at: 5062 275 Size: 1451 Fragments arranged by size: 1451 1413 1099 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2572 2602 1178 Size: 30 165 30 1169 30 4814 Fragments arranged by size: 4814 1169 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6238 BseYI C'CCAG_C Cuts at: 3033 5441 5937 3033 Size: 2408 496 3334 Fragments arranged by size: 3334 2408 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2036 1163 Size: 573 300 5365 Fragments arranged by size: 5365 573 300 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3688 4449 5477 1540 Size: 275 186 1687 761 1028 2301 Fragments arranged by size: 2301 1687 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4299 4384 5455 4299 Size: 85 1071 5082 Fragments arranged by size: 5082 1071 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 BsmBI CGTCTCn'nnnn_ Cuts at: 3528 3528 Size: 6238 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3557 4299 4384 5455 1391 Size: 399 1767 742 85 1071 2174 Fragments arranged by size: 2174 1767 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 4127 5857 4127 Size: 1730 4508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2470 3593 844 Size: 1626 1123 3489 Fragments arranged by size: 3489 1626 1123 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4733 4907 65 Size: 1183 3485 174 1396 Fragments arranged by size: 3485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4882 5069 1700 Size: 110 3072 187 2869 Fragments arranged by size: 3072 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 4298 5310 4298 Size: 1012 5226 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6238 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstYI r'GATC_y Cuts at: 1972 3220 3328 3568 3935 4320 4337 5789 5875 Size: 1248 108 240 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 240 108 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2727 513 Size: 552 939 723 4024 Fragments arranged by size: 4024 939 723 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 3754 4548 4568 3754 Size: 794 20 5424 Fragments arranged by size: 5424 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 2181 2544 3127 3542 2181 Size: 363 583 415 4877 Fragments arranged by size: 4877 583 415 363 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3309 3685 Size: 54 1425 50 275 18 177 1302 376 Cuts at: 3685 4574 6203 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1302 889 376 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2145 3319 4168 1353 Size: 792 1174 849 3423 Fragments arranged by size: 3423 1174 849 792 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4301 4898 5685 5953 1239 Size: 393 2669 597 787 268 1524 Fragments arranged by size: 2669 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2521 2521 Size: 6238 EcoRI G'AATT_C Cuts at: 3547 3925 3547 Size: 378 5860 EcoRV GAT'ATC Cuts at: 2500 2500 Size: 6238 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 2309 5385 1678 Size: 631 3076 2531 Fragments arranged by size: 3076 2531 631 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 2489 2521 3355 Size: 32 135 32 712 32 173 32 834 Cuts at: 3355 3387 4879 4911 4953 4985 1373 Size: 32 1492 32 42 32 2626 Fragments arranged by size: 2626 1492 834 712 173 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2734 3190 3582 3828 669 Size: 988 281 796 456 392 246 3079 Fragments arranged by size: 3079 988 796 456 392 281 246 HindIII A'AGCT_T Cuts at: 748 2863 3047 3302 748 Size: 2115 184 255 3684 Fragments arranged by size: 3684 2115 255 184 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 3563 3563 Size: 6238 MfeI C'AATT_G Cuts at: 2625 2811 3837 2625 Size: 186 1026 5026 Fragments arranged by size: 5026 1026 186 MluI A'CGCG_T Cuts at: 6114 6114 Size: 6238 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2363 3411 5352 5536 1305 Size: 1058 1048 1941 184 2007 Fragments arranged by size: 2007 1941 1058 1048 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 3311 10 Size: 54 1425 343 177 1302 2937 Fragments arranged by size: 2937 1425 1302 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4196 4555 4714 518 Size: 648 3030 359 159 2042 Fragments arranged by size: 3030 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3654 4331 5479 5724 1606 Size: 2048 677 1148 245 2120 Fragments arranged by size: 2120 2048 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2727 513 Size: 552 939 723 4024 Fragments arranged by size: 4024 939 723 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NsiI A_TGCA'T Cuts at: 2482 2482 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 3596 5141 1132 Size: 97 2367 1545 2229 Fragments arranged by size: 2367 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 2845 1108 Size: 1737 4501 PfoI T'CCnGG_A Cuts at: 3271 3271 Size: 6238 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2521 2521 Size: 6238 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PspXI vC'TCGA_Gb Cuts at: 2277 2277 Size: 6238 PstI C_TGCA'G Cuts at: 830 3590 830 Size: 2760 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 SalI G'TCGA_C Cuts at: 1936 3580 1936 Size: 1644 4594 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 4486 6198 4486 Size: 1712 4526 SfcI C'TryA_G Cuts at: 826 1045 3586 4721 5402 5593 826 Size: 219 2541 1135 681 191 1471 Fragments arranged by size: 2541 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2277 2765 4342 5243 5505 820 Size: 197 926 334 488 1577 901 262 1553 Fragments arranged by size: 1577 1553 926 901 488 334 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3596 1132 Size: 2464 3774 SspI AAT'ATT Cuts at: 5 52 4162 5 Size: 47 4110 2081 Fragments arranged by size: 4110 2081 47 StuI AGG'CCT Cuts at: 1517 2071 1517 Size: 554 5684 StyI C'CwwG_G Cuts at: 513 1065 2004 2727 3677 513 Size: 552 939 723 950 3074 Fragments arranged by size: 3074 950 939 723 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2898 3522 4265 4282 4435 4620 5065 Size: 1255 1318 624 743 17 153 185 445 Cuts at: 5065 325 Size: 1498 Fragments arranged by size: 1498 1318 1255 743 624 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4484 6196 96 Size: 274 80 33 51 1443 2507 1712 138 Fragments arranged by size: 2507 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2780 2812 6198 6230 2780 Size: 32 3386 32 2788 Fragments arranged by size: 3386 2788 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 2277 1943 Size: 334 5904 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 2334 2829 4367 2334 Size: 495 1538 4205 Fragments arranged by size: 4205 1538 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI