################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BanI BbeI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI FbaI FseI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 202 CGCG CG^CG AccIII 1355 TCCGGA T^CCGGA AflIII 528 ACGTGT A^C[GA][CT]GT AspI 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 644 TGCGCA TGC^GCA BbsI 838 GAAGAC GAAGAC(2/6) BcgI 1610 GCAAGATTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 462 AGATCT A^GATCT BpuAI 838 GAAGAC GAAGAC(2/6) BsaWI 1355 TCCGGA [AT]^CCGG[AT] BseAI 1355 TCCGGA T^CCGGA BsmI 1743 GAATGC GAATGC(1/-1) BspEI 1355 TCCGGA T^CCGGA BspHI 1208 TCATGA T^CATGA BsrBI 85 GAGCGG CCGCTC(-3/-3) BsrFI 290 ACCGGC [GA]^CCGG[CT] BstEII 1557 GGTGACC G^GT[ACGT]ACC BstPI 1557 GGTGACC G^GT[ACGT]ACC BstUI 202 CGCG CG^CG CfoI 645 GCGC GCG^C Cfr10I 290 ACCGGC [GA]^CCGG[CT] DraI 865 TTTAAA TTT^AAA DraIII 1022 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 438 CCATGG C^C[GA][CT]GG EaeI 137 CGGCCA [CT]^GGCC[GA] EcoO65I 1557 GGTGACC G^GT[ACGT]ACC EcoRV 748 GATATC GAT^ATC EcoT14I 438 CCATGG C^C[AT][AT]GG EcoT22I 1538 ATGCAT ATGCA^T FspI 644 TGCGCA TGC^GCA HhaI 645 GCGC GCG^C HinP1I 645 GCGC G^CGC MaeII 528 ACGT A^CGT MroI 1355 TCCGGA T^CCGGA MvnI 202 CGCG CG^CG NcoI 438 CCATGG C^CATGG NsiI 1538 ATGCAT ATGCA^T PflFI 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 624 GACTC GAGTC(4/5) PpuMI 1621 GGGTCCC [GA]G^G[AT]CC[CT] PshAI 260 GACAGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1208 TCATGA T^CATGA ScaI 629 AGTACT AGT^ACT SmlI 1780 CTCAAG C^T[CT][GA]AG SphI 1421 GCATGC GCATG^C SspI 1095 AATATT AAT^ATT StyI 438 CCATGG C^C[AT][AT]GG TaqI 152 TCGA T^CGA Tth111I 1222 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 152 TCGA T^CGA XbaI 1833 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 862 GAATTT [GA]^AATT[CT] 1786 AAATTT AfaI 212 GTAC GT^AC 630 GTAC AocI 351 CCTGAGG CC^T[ACGT]AGG 472 CCTCAGG ApaI 77 GGGCCC GGGCC^C 845 GGGCCC ApoI 862 GAATTT [GA]^AATT[CT] 1786 AAATTT AvaII 1344 GGTCC G^G[AT]CC 1622 GGTCC BfaI 1478 CTAG C^TAG 1834 CTAG BsaI 562 GAGACC GGTCTC(1/5) 608 GAGACC BstAPI 118 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1021 GCACTGAGTGC Bsu36I 351 CCTGAGG CC^T[ACGT]AGG 472 CCTCAGG Eco81I 351 CCTGAGG CC^T[ACGT]AGG 472 CCTCAGG MaeI 1478 CTAG C^TAG 1834 CTAG MslI 439 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1541 CATTTTGGTG NspI 47 ACATGC [GA]CATG^[CT] 1421 GCATGC PstI 116 CTGCAG CTGCA^G 1390 CTGCAG RsaI 212 GTAC GT^AC 630 GTAC SmaI 74 CCCGGG CCC^GGG 80 CCCGGG StuI 549 AGGCCT AGG^CCT 1386 AGGCCT TfiI 1331 GAATC G^A[AT]TC 1858 GAATC XcmI 1534 CCAGATGCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1562 CCATGACAACTGTGG XmaCI 74 CCCGGG C^CCGGG 80 CCCGGG XmaI 74 CCCGGG C^CCGGG 80 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AluI AlwI AlwNI AspHI AvaI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI XhoII