(Circular) (Six-base) MAPSORT of: pFN21AE4181.seq Check: 9925 from: 1 to: 6664 pFN21AE4181 6664 bp With 182 enzymes: SgfI * March 28, 2012 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4474 278 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4007 1937 Size: 2070 4594 Acc65I G'GTAC_C Cuts at: 3985 3985 Size: 6664 AclI AA'CG_TT Cuts at: 1563 4791 5164 1563 Size: 3228 373 3063 Fragments arranged by size: 3228 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2747 3051 4727 6111 6379 1632 Size: 1115 304 1676 1384 268 1917 Fragments arranged by size: 1917 1676 1384 1115 304 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6467 AflIII A'CryG_T Cuts at: 1203 2533 5563 6540 1203 Size: 1330 3030 977 1327 Fragments arranged by size: 3030 1330 1327 977 AgeI A'CCGG_T Cuts at: 5495 5495 Size: 6664 AhdI GACnn_n'nnGTC Cuts at: 5393 5393 Size: 6664 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6664 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6664 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2759 2791 2759 Size: 32 6632 AlwNI CAG_nnn'CTG Cuts at: 2129 2132 2153 2523 3403 4407 5979 2129 Size: 3 21 370 880 1004 1572 2814 Fragments arranged by size: 2814 1572 1004 880 370 21 3 ApaI G_GGCC'C Cuts at: 2086 2854 2086 Size: 768 5896 ApaLI G'TGCA_C Cuts at: 4721 5877 4721 Size: 1156 5508 ApoI r'AATT_y Cuts at: 1493 2867 3791 3973 4199 4351 6460 1493 Size: 1374 924 182 226 152 2109 1697 Fragments arranged by size: 2109 1697 1374 924 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 5218 160 Size: 872 4186 1606 Fragments arranged by size: 4186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 AvaI C'yCGr_G Cuts at: 1870 1943 2079 2085 2858 3989 1870 Size: 73 136 6 773 1131 4545 Fragments arranged by size: 4545 1131 773 136 73 6 BamHI G'GATC_C Cuts at: 3994 4361 3994 Size: 367 6297 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3985 5440 618 Size: 325 217 627 2198 1455 1842 Fragments arranged by size: 2198 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2036 2086 2186 2854 2912 3803 3983 1391 Size: 645 50 100 668 58 891 180 4072 Fragments arranged by size: 4072 891 668 645 180 100 58 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2850 928 Size: 971 951 4742 Fragments arranged by size: 4742 971 951 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3604 3638 4855 4889 1903 Size: 34 1667 34 1217 34 3678 Fragments arranged by size: 3678 1667 1217 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4556 5766 4556 Size: 1210 5454 BclI T'GATC_A Cuts at: 1548 1548 Size: 6664 BfrBI ATG'CAT Cuts at: 3545 3545 Size: 6664 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5275 136 Size: 107 122 71 631 605 3603 1525 Fragments arranged by size: 3603 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2467 6659 2467 Size: 4192 2472 BlpI GC'TnA_GC Cuts at: 4081 4081 Size: 6664 Bme1580I G_kGCm'C Cuts at: 1790 2086 2854 3096 3266 4725 5881 1790 Size: 296 768 242 170 1459 1156 2573 Fragments arranged by size: 2573 1459 1156 768 296 242 170 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6664 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2328 2584 3269 Size: 416 53 83 399 24 911 256 685 Cuts at: 3269 3446 4395 5353 442 Size: 177 949 958 1753 Fragments arranged by size: 1753 958 949 911 685 416 399 256 177 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6664 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2121 2121 Size: 6664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2095 2896 3101 3361 5324 1239 Size: 856 801 205 260 1963 2579 Fragments arranged by size: 2579 1963 856 801 260 205 Bpu10I CC'TnA_GC Cuts at: 2035 2035 Size: 6664 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3770 4789 5654 5952 3770 Size: 1019 865 298 4482 Fragments arranged by size: 4482 1019 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2561 2607 5327 882 Size: 617 1062 46 2720 2219 Fragments arranged by size: 2720 2219 1062 617 46 BsaAI yAC'GTr Cuts at: 493 1204 4455 493 Size: 711 3251 2702 Fragments arranged by size: 3251 2702 711 BsaBI GATnn'nnATC Cuts at: 6658 6658 Size: 6664 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4471 4853 5488 275 Size: 53 83 186 936 2938 382 635 1451 Fragments arranged by size: 2938 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3360 5097 5495 5769 5916 6327 3360 Size: 1737 398 274 147 411 3697 Fragments arranged by size: 3697 1737 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2405 2435 2759 2786 2789 Size: 30 165 30 1002 30 324 27 3 Cuts at: 2789 2816 3218 3248 3872 3902 1178 Size: 27 402 30 624 30 3940 Fragments arranged by size: 3940 1002 624 402 324 165 30 30 30 30 30 27 27 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2269 2272 2360 2387 2390 2402 2405 2408 Size: 1083 3 88 27 3 12 3 3 Cuts at: 2408 2417 2426 2780 2807 2879 3896 1186 Size: 9 9 354 27 72 1017 3954 Fragments arranged by size: 3954 1083 1017 354 88 72 27 27 12 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2681 3185 3276 5867 6363 2681 Size: 504 91 2591 496 2982 Fragments arranged by size: 2982 2591 504 496 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6091 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4114 4875 5903 1540 Size: 275 186 2113 761 1028 2301 Fragments arranged by size: 2301 2113 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2036 2912 3803 4725 4810 5881 2036 Size: 876 891 922 85 1071 2819 Fragments arranged by size: 2819 1071 922 891 876 85 BsmI GAATG_Cn' Cuts at: 3754 4175 4268 3754 Size: 421 93 6150 Fragments arranged by size: 6150 421 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2086 2186 2854 2912 3096 3266 Size: 399 246 50 100 668 58 184 170 Cuts at: 3266 3803 3983 4725 4810 5881 1391 Size: 537 180 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 537 399 246 184 180 170 100 85 58 50 BspEI T'CCGG_A Cuts at: 3360 6327 3360 Size: 2967 3697 BspHI T'CATG_A Cuts at: 3213 4553 6283 3213 Size: 1340 1730 3594 Fragments arranged by size: 3594 1730 1340 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4019 844 Size: 3175 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2092 4551 1394 Size: 429 269 2459 3507 Fragments arranged by size: 3507 2459 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 2022 2314 2659 3301 3639 3657 5159 Size: 1183 774 292 345 642 338 18 1502 Cuts at: 5159 5333 65 Size: 174 1396 Fragments arranged by size: 1502 1396 1183 774 642 345 338 292 174 18 BsrFI r'CCGG_y Cuts at: 1700 1810 2295 5308 5495 1700 Size: 110 485 3013 187 2869 Fragments arranged by size: 3013 2869 485 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6664 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6664 BssSI C'ACGA_G Cuts at: 4724 5736 4724 Size: 1012 5652 BstAPI GCAn_nnn'nTGC Cuts at: 794 2129 3032 794 Size: 1335 903 4426 Fragments arranged by size: 4426 1335 903 BstBI TT'CG_AA Cuts at: 5468 5468 Size: 6664 BstEII G'GTnAC_C Cuts at: 1184 3562 1184 Size: 2378 4286 BstYI r'GATC_y Cuts at: 1972 2352 2467 3013 3363 3399 3994 4361 4746 Size: 380 115 546 350 36 595 367 385 Cuts at: 4746 4763 6215 6301 6313 6659 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 595 546 385 380 367 350 346 115 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2357 2478 2357 Size: 121 6543 BtgI C'CryG_G Cuts at: 513 1065 2004 2443 513 Size: 552 939 439 4734 Fragments arranged by size: 4734 939 552 439 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5925 Fragments arranged by size: 5925 683 56 BtsI GCAGTG_nn' Cuts at: 4180 4974 4994 4180 Size: 794 20 5850 Fragments arranged by size: 5850 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6664 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3930 3965 529 Size: 35 3366 35 3228 Fragments arranged by size: 3366 3228 35 35 DraI TTT'AAA Cuts at: 2872 3968 2872 Size: 1096 5568 DraIII CAC_nnn'GTG Cuts at: 3032 3032 Size: 6664 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5671 809 Size: 507 4355 1802 Fragments arranged by size: 4355 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 4111 5000 Size: 54 1425 50 275 18 312 1969 889 Cuts at: 5000 6629 8 Size: 1629 43 Fragments arranged by size: 1969 1629 1425 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4111 1537 Size: 275 2299 4090 Fragments arranged by size: 4090 2299 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2403 2723 2832 3203 3464 4594 1353 Size: 1041 9 320 109 371 261 1130 3423 Fragments arranged by size: 3423 1130 1041 371 320 261 109 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3521 5251 5625 5771 387 Size: 1064 138 1932 1730 374 146 1280 Fragments arranged by size: 1932 1730 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2034 2910 3801 2034 Size: 876 891 4897 Fragments arranged by size: 4897 891 876 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2095 2747 2896 3051 3101 3361 4727 Size: 393 463 652 149 155 50 260 1366 Cuts at: 4727 5324 6111 6379 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1366 787 652 597 463 393 268 260 155 149 50 EcoO109I rG'GnC_Cy Cuts at: 2211 2797 2850 2851 3627 2211 Size: 586 53 1 776 5248 Fragments arranged by size: 5248 776 586 53 1 EcoRI G'AATT_C Cuts at: 3973 4351 3973 Size: 378 6286 EcoRV GAT'ATC Cuts at: 2755 2755 Size: 6664 FspI TGC'GCA Cuts at: 2651 5170 2651 Size: 2519 4145 HaeII r_GCGC'y Cuts at: 1678 5811 1678 Size: 4133 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2899 2931 3218 3250 5305 Size: 32 135 32 1327 32 287 32 2055 Cuts at: 5305 5337 5379 5411 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2055 1327 287 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4008 4254 669 Size: 988 281 2070 246 3079 Fragments arranged by size: 3079 2070 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6664 HpaI GTT'AAC Cuts at: 4254 4254 Size: 6664 KpnI G_GTAC'C Cuts at: 3989 3989 Size: 6664 MfeI C'AATT_G Cuts at: 4263 4263 Size: 6664 MluI A'CGCG_T Cuts at: 6540 6540 Size: 6664 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2231 5778 5962 1305 Size: 926 3547 184 2007 Fragments arranged by size: 3547 2007 926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4842 Fragments arranged by size: 4842 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2448 3550 4622 4981 5140 518 Size: 648 1282 1102 1072 359 159 2042 Fragments arranged by size: 2042 1282 1102 1072 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2120 2129 2141 2150 2269 4080 4757 5905 Size: 514 9 12 9 119 1811 677 1148 Cuts at: 5905 6150 1606 Size: 245 2120 Fragments arranged by size: 2120 1811 1148 677 514 245 119 12 9 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2443 513 Size: 552 939 439 4734 Fragments arranged by size: 4734 939 552 439 NdeI CA'TA_TG Cuts at: 387 387 Size: 6664 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6664 NotI GC'GGCC_GC Cuts at: 4111 4111 Size: 6664 NsiI A_TGCA'T Cuts at: 3547 3547 Size: 6664 NspI r_CATG'y Cuts at: 1132 1229 2056 3430 4022 5567 1132 Size: 97 827 1374 592 1545 2229 Fragments arranged by size: 2229 1545 1374 827 592 97 PasI CC'CwG_GG Cuts at: 1348 3817 1348 Size: 2469 4195 PciI A'CATG_T Cuts at: 5563 5563 Size: 6664 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6664 PmeI GTTT'AAAC Cuts at: 3968 3968 Size: 6664 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2759 2791 4733 4765 6246 6278 2759 Size: 32 1942 32 1481 32 3145 Fragments arranged by size: 3145 1942 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3627 3627 Size: 6664 PshAI GACnn'nnGTC Cuts at: 1613 2269 1613 Size: 656 6008 PsiI TTA'TAA Cuts at: 4234 4234 Size: 6664 PspOMI G'GGCC_C Cuts at: 2082 2850 2082 Size: 768 5896 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2155 2187 2155 Size: 32 6632 PstI C_TGCA'G Cuts at: 830 2125 3399 4016 830 Size: 1295 1274 617 3478 Fragments arranged by size: 3478 1295 1274 617 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6664 PvuII CAG'CTG Cuts at: 2120 2129 2150 2120 Size: 9 21 6634 Fragments arranged by size: 6634 21 9 SacI G_AGCT'C Cuts at: 2036 2912 3803 2036 Size: 876 891 4897 Fragments arranged by size: 4897 891 876 SalI G'TCGA_C Cuts at: 1936 4006 1936 Size: 2070 4594 SanDI GG'GwC_CC Cuts at: 3627 3627 Size: 6664 SbfI CC_TGCA'GG Cuts at: 4016 4016 Size: 6664 ScaI AGT'ACT Cuts at: 2636 4912 6624 2636 Size: 2276 1712 2676 Fragments arranged by size: 2676 2276 1712 SfcI C'TryA_G Cuts at: 826 1045 2121 2428 2500 2515 3395 3606 4012 Size: 219 1076 307 72 15 880 211 406 Cuts at: 4012 5147 5828 6019 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1076 880 681 406 307 219 211 191 72 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6664 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6664 SmaI CCC'GGG Cuts at: 1872 2081 2087 3991 1872 Size: 209 6 1904 4545 Fragments arranged by size: 4545 1904 209 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 3785 4768 5669 5931 820 Size: 197 926 1842 983 901 262 1553 Fragments arranged by size: 1842 1553 983 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4455 493 Size: 3962 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6664 SphI G_CATG'C Cuts at: 1132 3430 4022 1132 Size: 2298 592 3774 Fragments arranged by size: 3774 2298 592 SrfI GCCC'GGGC Cuts at: 2081 2081 Size: 6664 SspI AAT'ATT Cuts at: 5 52 3102 4588 5 Size: 47 3050 1486 2081 Fragments arranged by size: 3050 2081 1486 47 StuI AGG'CCT Cuts at: 1517 2556 3393 1517 Size: 1039 837 4788 Fragments arranged by size: 4788 1039 837 StyI C'CwwG_G Cuts at: 513 1065 2004 2443 4103 513 Size: 552 939 439 1660 3074 Fragments arranged by size: 3074 1660 939 552 439 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4691 4708 4861 5046 5491 325 Size: 1255 3111 17 153 185 445 1498 Fragments arranged by size: 3111 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2634 4910 6622 Size: 274 80 33 51 1443 657 2276 1712 Cuts at: 6622 96 Size: 138 Fragments arranged by size: 2276 1712 1443 657 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3854 3872 3886 3904 6624 6656 3854 Size: 18 14 18 2720 32 3862 Fragments arranged by size: 3862 2720 32 18 18 14 Tth111I GACn'n_nGTC Cuts at: 1316 3230 1316 Size: 1914 4750 XbaI T'CTAG_A Cuts at: 3838 4000 3838 Size: 162 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3546 3574 3546 Size: 28 6636 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6664 XmaI C'CCGG_G Cuts at: 1870 2079 2085 3989 1870 Size: 209 6 1904 4545 Fragments arranged by size: 4545 1904 209 6 XmnI GAAnn'nnTTC Cuts at: 4793 4793 Size: 6664 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4472 276 Size: 53 83 186 936 2938 2468 Fragments arranged by size: 2938 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI RsrII SacII SapI SexAI SfiI SfoI SwaI