Restriction map for
pFN21AE5483
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI HpaII KasI KpnI
KspI MfeI MluI MroI MseI
MspI MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AocI 295 CCTAAGG CC^T[ACGT]AGG
BamHI 781 GGATCC G^GATCC
BanII 229 GAGCCC G[GA]GC[CT]^C
BbsI 730 GTCTTC GAAGAC(2/6)
BlpI 967 GCTAAGC GC^T[ACGT]AGC
BpmI 118 CTGGAG CTGGAG(16/14)
Bpu1102I 967 GCTAAGC GC^T[ACGT]AGC
BpuAI 730 GTCTTC GAAGAC(2/6)
BsaBI 567 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 553 CTGCAC GTGCAG(16/14)
BsmFI 814 GTCCC GGGAC(10/14)
BspMI 551 ACCTGC ACCTGC(4/8)
BsrBI 756 CCGCTC CCGCTC(-3/-3)
BstAPI 860 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 295 CCTAAGG CC^T[ACGT]AGG
CelII 967 GCTAAGC GC^T[ACGT]AGC
DsaI 407 CCATGG C^C[GA][CT]GG
Eco81I 295 CCTAAGG CC^T[ACGT]AGG
EcoNI 207 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 628 GATATC GAT^ATC
FauI 755 CCCGC CCCGC(4/6)
HaeII 15 GGCGCT [GA]GCGC^[CT]
HphI 9 GGTGA GGTGA(8/7)
MaeII 899 ACGT A^CGT
MamI 567 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 659 CAGCTG C[AC]G^C[GT]G
NcoI 407 CCATGG C^CATGG
NlaIV 781 GGATCC GG[ACGT]^[ACGT]CC
PflMI 308 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 659 CAGCTG CAG^CTG
SapI 664 GCTCTTC GCTCTTC(1/4)
SfcI 840 CTACAG C^T[GA][CT]AG
StuI 678 AGGCCT AGG^CCT
Van91I 308 CCAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AluI 511 AGCT AG^CT
660 AGCT
AspHI 547 GAGCAC G[AT]GC[AT]^C
595 GAGCAC
BfaI 319 CTAG C^TAG
682 CTAG
BsiHKAI 547 GAGCAC G[AT]GC[AT]^C
595 GAGCAC
Bsp1407I 161 TGTACA T^GTACA
236 TGTACA
BsrGI 161 TGTACA T^GTACA
236 TGTACA
BstYI 781 GGATCC [GA]^GATC[CT]
997 GGATCT
CfoI 16 GCGC GCG^C
820 GCGC
DraII 299 AGGCCCT [GA]G^G[ACGT]CC[CT]
764 AGGCCCT
EcoO109I 299 AGGCCCT [GA]G^G[ACGT]CC[CT]
764 AGGCCCT
EcoT14I 407 CCATGG C^C[AT][AT]GG
761 CCAAGG
HhaI 16 GCGC GCG^C
820 GCGC
HinP1I 16 GCGC G^CGC
820 GCGC
MaeI 319 CTAG C^TAG
682 CTAG
MaeIII 454 GTCAC ^GT[ACGT]AC
490 GTCAC
MflI 781 GGATCC [GA]^GATC[CT]
997 GGATCT
MwoI 83 GCATAGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
860 GCATCCCATGC
SmlI 544 CTTGAG C^T[CT][GA]AG
746 CTCAAG
SspBI 161 TGTACA T^GTACA
236 TGTACA
StyI 407 CCATGG C^C[AT][AT]GG
761 CCAAGG
Tsp45I 454 GTCAC ^GT[GC]AC
490 GTCAC
Tsp509I 195 AATT ^AATT
310 AATT
XhoII 781 GGATCC [GA]^GATC[CT]
997 GGATCT
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AfaI AlwI BalI BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII Fnu4HI FokI HaeIII
ItaI Ksp632I MboI MboII MluNI
MnlI MscI MslI MvaI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI TseI TspRI