################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BbvCI BciVI BclI BfrI BlnI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII Eco47III EcoRI EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KspI MfeI MluI MroI MseI MunI NaeI NarI NgoMIV NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SwaI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 1028 GGTACC G^GTACC AhdI 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1651 GTGCAC G^TGCAC ApaI 49 GGGCCC GGGCC^C ApaLI 1651 GTGCAC G^TGCAC Asp700I 357 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1028 GGTACC G^GTACC AspEI 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 134 TGGCCA TGG^CCA BbrPI 289 CACGTG CAC^GTG BbsI 66 GAAGAC GAAGAC(2/6) BcgI 1911 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1598 AGATCT A^GATCT BpuAI 66 GAAGAC GAAGAC(2/6) BsaAI 289 CACGTG [CT]AC^GT[GA] BsaBI 198 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1016 ACCGGA [AT]^CCGG[AT] BsiEI 579 CGGCCG CG[GA][CT]^CG BssHII 26 GCGCGC G^CGCGC BstAPI 957 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1287 GGTGACC G^GT[ACGT]ACC BstPI 1287 GGTGACC G^GT[ACGT]ACC BstXI 1199 CCATTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1524 GACACCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 579 CGGCCG C^GGCCG Eam1105I 961 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 307 CTCTTC CTCTTC(1/4) EclXI 579 CGGCCG C^GGCCG Eco52I 579 CGGCCG C^GGCCG EcoO65I 1287 GGTGACC G^GT[ACGT]ACC EcoRV 1774 GATATC GAT^ATC HincII 148 GTCAAC GT[CT]^[GA]AC HindII 148 GTCAAC GT[CT]^[GA]AC KpnI 1028 GGTACC GGTAC^C Ksp632I 307 CTCTTC CTCTTC(1/4) MamI 198 GATCGCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 134 TGGCCA TGG^CCA MscI 134 TGGCCA TGG^CCA NcoI 1145 CCATGG C^CATGG NruI 200 TCGCGA TCG^CGA PflMI 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 289 CACGTG CAC^GTG PmlI 289 CACGTG CAC^GTG PshAI 1338 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 306 GCTCTTC GCTCTTC(1/4) SbfI 492 CCTGCAGG CCTGCA^GG SmaI 1283 CCCGGG CCC^GGG SphI 914 GCATGC GCATG^C Sse8387I 492 CCTGCAGG CCTGCA^GG SspI 266 AATATT AAT^ATT StuI 1254 AGGCCT AGG^CCT TfiI 1668 GATTC G^A[AT]TC Tsp509I 1989 AATT ^AATT Van91I 126 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1943 CCACTAACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1283 CCCGGG C^CCGGG XmaI 1283 CCCGGG C^CCGGG XmnI 357 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AocI 343 CCTAAGG CC^T[ACGT]AGG 1250 CCTCAGG BfaI 283 CTAG C^TAG 781 CTAG BsgI 241 CTGCAC GTGCAG(16/14) 859 GTGCAG BsrBI 867 CCGCTC CCGCTC(-3/-3) 190 GAGCGG BsrI 924 ACTGG ACTGG(1/-1) 1673 CCAGT Bsu36I 343 CCTAAGG CC^T[ACGT]AGG 1250 CCTCAGG DsaI 1145 CCATGG C^C[GA][CT]GG 1727 CCACGG Eco81I 343 CCTAAGG CC^T[ACGT]AGG 1250 CCTCAGG EcoNI 1301 CCTCACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1581 CCTGGAGAAGG MaeI 283 CTAG C^TAG 781 CTAG MslI 1146 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1200 CATTGAGATG NdeI 408 CATATG CA^TATG 801 CATATG NspI 701 ACATGC [GA]CATG^[CT] 914 GCATGC PstI 493 CTGCAG CTGCA^G 955 CTGCAG SacI 304 GAGCTC GAGCT^C 1629 GAGCTC SexAI 275 ACCTGGT A^CC[AT]GGT 317 ACCTGGT SfcI 493 CTGCAG C^T[GA][CT]AG 955 CTGCAG TspRI 112 CACTG CA[GC]TG[ACGT][ACGT]^ 923 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BglI BlpI BmyI BpmI Bpu1102I BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TthHB8I XhoII