(Circular) (Six-base) MAPSORT of: pFN21AE3695.seq Check: 4583 from: 1 to: 6709 pFN21AE3695 6709 bp With 182 enzymes: SgfI * March 28, 2012 13:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2135 2135 Size: 6709 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4519 278 Size: 53 83 186 936 2983 2468 Fragments arranged by size: 2983 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4052 1937 Size: 2115 4594 Acc65I G'GTAC_C Cuts at: 3033 4030 3033 Size: 997 5712 AclI AA'CG_TT Cuts at: 1563 4836 5209 1563 Size: 3273 373 3063 Fragments arranged by size: 3273 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2234 4772 6156 6424 1632 Size: 602 2538 1384 268 1917 Fragments arranged by size: 2538 1917 1384 602 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6512 AflIII A'CryG_T Cuts at: 1203 2293 2295 2598 5608 6585 1203 Size: 1090 2 303 3010 977 1327 Fragments arranged by size: 3010 1327 1090 977 303 2 AgeI A'CCGG_T Cuts at: 5540 5540 Size: 6709 AhdI GACnn_n'nnGTC Cuts at: 2971 5438 2971 Size: 2467 4242 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2972 1184 Size: 1788 4921 AlwNI CAG_nnn'CTG Cuts at: 2677 2740 2968 3904 4452 6024 2677 Size: 63 228 936 548 1572 3362 Fragments arranged by size: 3362 1572 936 548 228 63 ApaI G_GGCC'C Cuts at: 2058 2058 Size: 6709 ApaLI G'TGCA_C Cuts at: 3656 4766 5922 3656 Size: 1110 1156 4443 Fragments arranged by size: 4443 1156 1110 ApoI r'AATT_y Cuts at: 1493 4018 4244 4396 6505 1493 Size: 2525 226 152 2109 1697 Fragments arranged by size: 2525 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5263 160 Size: 872 4231 1606 Fragments arranged by size: 4231 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 AvaI C'yCGr_G Cuts at: 1870 1943 2555 3037 3288 4034 1870 Size: 73 612 482 251 746 4545 Fragments arranged by size: 4545 746 612 482 251 73 BamHI G'GATC_C Cuts at: 4039 4406 4039 Size: 367 6342 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3033 3187 3248 3421 4030 Size: 325 217 627 1246 154 61 173 609 Cuts at: 4030 5485 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1246 627 609 325 217 173 154 61 BanII G_rGCy'C Cuts at: 1391 2058 2313 2644 2835 3371 3470 3638 4028 Size: 667 255 331 191 536 99 168 390 Cuts at: 4028 1391 Size: 4072 Fragments arranged by size: 4072 667 536 390 331 255 191 168 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2078 928 Size: 971 179 5559 Fragments arranged by size: 5559 971 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3905 3939 4900 4934 1903 Size: 34 1968 34 961 34 3678 Fragments arranged by size: 3678 1968 961 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4601 5811 4601 Size: 1210 5499 BclI T'GATC_A Cuts at: 1548 1548 Size: 6709 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2062 3196 3375 Size: 107 122 71 631 605 390 1134 179 Cuts at: 3375 5320 136 Size: 1945 1525 Fragments arranged by size: 1945 1525 1134 631 605 390 179 122 107 71 BglII A'GATC_T Cuts at: 3603 6704 3603 Size: 3101 3608 BlpI GC'TnA_GC Cuts at: 2168 3161 3905 4126 2168 Size: 993 744 221 4751 Fragments arranged by size: 4751 993 744 221 Bme1580I G_kGCm'C Cuts at: 1790 2058 3426 3660 4770 5926 1790 Size: 268 1368 234 1110 1156 2573 Fragments arranged by size: 2573 1368 1156 1110 268 234 BmgBI CAC'GTC Cuts at: 1318 2236 2599 2908 1318 Size: 918 363 309 5119 Fragments arranged by size: 5119 918 363 309 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2939 3672 4440 Size: 416 53 83 399 24 1522 733 768 Cuts at: 4440 5398 442 Size: 958 1753 Fragments arranged by size: 1753 1522 958 768 733 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6709 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2699 2909 3448 3608 5369 1239 Size: 848 612 210 539 160 1761 2579 Fragments arranged by size: 2579 1761 848 612 539 210 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4834 5699 5997 4834 Size: 865 298 5546 Fragments arranged by size: 5546 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2516 2937 3237 3878 5372 882 Size: 617 1017 421 300 641 1494 2219 Fragments arranged by size: 2219 1494 1017 641 617 421 300 BsaAI yAC'GTr Cuts at: 493 1204 2296 4500 493 Size: 711 1092 2204 2702 Fragments arranged by size: 2702 2204 1092 711 BsaBI GATnn'nnATC Cuts at: 2207 6703 2207 Size: 4496 2213 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2345 2492 2613 2766 Size: 53 83 186 936 812 147 121 153 Cuts at: 2766 3506 4516 4898 5533 275 Size: 740 1010 382 635 1451 Fragments arranged by size: 1451 1010 936 812 740 635 382 186 153 147 121 83 53 BsaWI w'CCGG_w Cuts at: 3021 5142 5540 5814 5961 6372 3021 Size: 2121 398 274 147 411 3358 Fragments arranged by size: 3358 2121 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2393 2423 3164 3194 3503 Size: 30 165 30 990 30 741 30 309 Cuts at: 3503 3533 1178 Size: 30 4354 Fragments arranged by size: 4354 990 741 309 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2993 2996 3002 3244 3527 3611 1186 Size: 1807 3 6 242 283 84 4284 Fragments arranged by size: 4284 1807 283 242 84 6 3 BseYI C'CCAG_C Cuts at: 2077 2107 2882 3216 3407 3857 3897 3930 5912 Size: 30 775 334 191 450 40 33 1982 Cuts at: 5912 6408 2077 Size: 496 2378 Fragments arranged by size: 2378 1982 775 496 450 334 191 40 33 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2231 2885 1163 Size: 573 495 654 4987 Fragments arranged by size: 4987 654 573 495 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2587 4159 4920 5948 1540 Size: 275 186 586 1572 761 1028 2301 Fragments arranged by size: 2301 1572 1028 761 586 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2313 2793 3162 3638 3660 3911 4770 4855 Size: 84 480 369 476 22 251 859 85 Cuts at: 4855 5926 2229 Size: 1071 3012 Fragments arranged by size: 3012 1071 859 480 476 369 251 85 84 22 BsmI GAATG_Cn' Cuts at: 4220 4313 4220 Size: 93 6616 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2229 2313 2644 2793 2835 3162 Size: 399 268 171 84 331 149 42 327 Cuts at: 3162 3371 3426 3470 3638 3660 3911 4028 4770 Size: 209 55 44 168 22 251 117 742 Cuts at: 4770 4855 5926 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 331 327 268 251 209 171 168 149 117 85 84 55 44 42 22 BspEI T'CCGG_A Cuts at: 6372 6372 Size: 6709 BspHI T'CATG_A Cuts at: 4598 6328 4598 Size: 1730 4979 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2135 2491 2976 3066 4064 844 Size: 1291 356 485 90 998 3489 Fragments arranged by size: 3489 1291 998 485 356 90 BsrBI CCG'CTC Cuts at: 1394 1823 2197 2874 4596 1394 Size: 429 374 677 1722 3507 Fragments arranged by size: 3507 1722 677 429 374 BsrDI GCAATG_nn' Cuts at: 65 1248 5204 5378 65 Size: 1183 3956 174 1396 Fragments arranged by size: 3956 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 3117 3760 5353 5540 1700 Size: 110 251 1056 643 1593 187 2869 Fragments arranged by size: 2869 1593 1056 643 251 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6709 BssHII G'CGCG_C Cuts at: 1925 2031 1925 Size: 106 6603 BssSI C'ACGA_G Cuts at: 4769 5781 4769 Size: 1012 5697 BstAPI GCAn_nnn'nTGC Cuts at: 794 2968 794 Size: 2174 4535 BstBI TT'CG_AA Cuts at: 5513 5513 Size: 6709 BstEII G'GTnAC_C Cuts at: 1184 3292 1184 Size: 2108 4601 BstXI CCAn_nnnn'nTGG Cuts at: 3211 3211 Size: 6709 BstYI r'GATC_y Cuts at: 1972 2425 2868 3603 4039 4406 4791 4808 6260 Size: 453 443 735 436 367 385 17 1452 Cuts at: 6260 6346 6358 6704 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 735 453 443 436 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2349 3256 2349 Size: 907 5802 BtgI C'CryG_G Cuts at: 513 1065 3150 3732 513 Size: 552 2085 582 3490 Fragments arranged by size: 3490 2085 582 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2458 487 Size: 683 56 1232 4738 Fragments arranged by size: 4738 1232 683 56 BtsI GCAGTG_nn' Cuts at: 2116 4225 5019 5039 2116 Size: 2109 794 20 3786 Fragments arranged by size: 3786 2109 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6709 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6674 DraI TTT'AAA Cuts at: 4013 4013 Size: 6709 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3535 5716 809 Size: 507 2219 2181 1802 Fragments arranged by size: 2219 2181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2584 3134 Size: 54 1425 50 275 18 309 445 550 Cuts at: 3134 3223 4156 5045 6674 8 Size: 89 933 889 1629 43 Fragments arranged by size: 1629 1425 933 889 550 445 309 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2584 4156 1537 Size: 275 772 1572 4090 Fragments arranged by size: 4090 1572 772 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 4639 1353 Size: 965 2321 3423 Fragments arranged by size: 3423 2321 965 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2609 5296 5670 5816 387 Size: 1064 138 1020 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 1020 374 146 138 EcoICRI GAG'CTC Cuts at: 2311 3636 2311 Size: 1325 5384 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2234 2699 2909 3448 3608 4772 Size: 393 455 147 465 210 539 160 1164 Cuts at: 4772 5369 6156 6424 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1164 787 597 539 465 455 393 268 210 160 147 EcoNI CCTnn'n_nnAGG Cuts at: 3310 3590 3310 Size: 280 6429 EcoO109I rG'GnC_Cy Cuts at: 2055 2535 2965 3055 3854 2055 Size: 480 430 90 799 4910 Fragments arranged by size: 4910 799 480 430 90 EcoRI G'AATT_C Cuts at: 4018 4396 4018 Size: 378 6331 EcoRV GAT'ATC Cuts at: 3781 3781 Size: 6709 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3475 3475 Size: 6709 FspI TGC'GCA Cuts at: 5215 5215 Size: 6709 HaeII r_GCGC'y Cuts at: 1678 5856 1678 Size: 4178 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2957 2989 3067 3099 3164 Size: 32 135 32 1385 32 78 32 65 Cuts at: 3164 3196 3503 3535 3664 3696 5350 5382 5424 Size: 32 307 32 129 32 1654 32 42 Cuts at: 5424 5456 1373 Size: 32 2626 Fragments arranged by size: 2626 1654 1385 307 135 129 78 65 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 4053 4299 669 Size: 988 281 217 1898 246 3079 Fragments arranged by size: 3079 1898 988 281 246 217 HindIII A'AGCT_T Cuts at: 748 748 Size: 6709 HpaI GTT'AAC Cuts at: 4299 4299 Size: 6709 KpnI G_GTAC'C Cuts at: 3037 4034 3037 Size: 997 5712 MfeI C'AATT_G Cuts at: 4308 4308 Size: 6709 MluI A'CGCG_T Cuts at: 6585 6585 Size: 6709 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2499 3394 3527 3546 5823 6007 1305 Size: 1194 895 133 19 2277 184 2007 Fragments arranged by size: 2277 2007 1194 895 184 133 19 MscI TGG'CCA Cuts at: 10 64 1489 1832 2141 10 Size: 54 1425 343 309 4578 Fragments arranged by size: 4578 1425 343 309 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3209 4667 5026 5185 518 Size: 648 1989 54 1458 359 159 2042 Fragments arranged by size: 2042 1989 1458 648 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2529 2674 3246 3901 4125 4802 5950 6195 Size: 923 145 572 655 224 677 1148 245 Cuts at: 6195 1606 Size: 2120 Fragments arranged by size: 2120 1148 923 677 655 572 245 224 145 NcoI C'CATG_G Cuts at: 513 1065 3150 513 Size: 552 2085 4072 Fragments arranged by size: 4072 2085 552 NdeI CA'TA_TG Cuts at: 387 2414 2807 387 Size: 2027 393 4289 Fragments arranged by size: 4289 2027 393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6709 NotI GC'GGCC_GC Cuts at: 4156 4156 Size: 6709 NruI TCG'CGA Cuts at: 2207 2207 Size: 6709 NspI r_CATG'y Cuts at: 1132 1229 2710 2923 4067 5612 1132 Size: 97 1481 213 1144 1545 2229 Fragments arranged by size: 2229 1545 1481 1144 213 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6709 PciI A'CATG_T Cuts at: 5608 5608 Size: 6709 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 1108 Size: 1029 5680 PfoI T'CCnGG_A Cuts at: 3015 3015 Size: 6709 PmeI GTTT'AAAC Cuts at: 4013 4013 Size: 6709 PmlI CAC'GTG Cuts at: 2296 2296 Size: 6709 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4778 4810 6291 6323 4778 Size: 32 1481 32 5164 Fragments arranged by size: 5164 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2535 2965 3055 2535 Size: 430 90 6189 Fragments arranged by size: 6189 430 90 PshAI GACnn'nnGTC Cuts at: 1613 3347 1613 Size: 1734 4975 PsiI TTA'TAA Cuts at: 4279 4279 Size: 6709 PspOMI G'GGCC_C Cuts at: 2054 2054 Size: 6709 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3809 3841 3809 Size: 32 6677 PstI C_TGCA'G Cuts at: 830 2502 2964 4061 830 Size: 1672 462 1097 3478 Fragments arranged by size: 3478 1672 1097 462 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6709 PvuII CAG'CTG Cuts at: 2529 2674 3901 2529 Size: 145 1227 5337 Fragments arranged by size: 5337 1227 145 SacI G_AGCT'C Cuts at: 2313 3638 2313 Size: 1325 5384 SalI G'TCGA_C Cuts at: 1936 4051 1936 Size: 2115 4594 SanDI GG'GwC_CC Cuts at: 2535 2535 Size: 6709 SapI GCTCTTCn'nnn_ Cuts at: 2318 2318 Size: 6709 SbfI CC_TGCA'GG Cuts at: 2502 4061 2502 Size: 1559 5150 ScaI AGT'ACT Cuts at: 4957 6669 4957 Size: 1712 4997 SexAI A'CCwGG_T Cuts at: 2280 2322 2280 Size: 42 6667 SfcI C'TryA_G Cuts at: 826 1045 2498 2960 4057 5192 5873 6064 826 Size: 219 1453 462 1097 1135 681 191 1471 Fragments arranged by size: 1471 1453 1135 1097 681 462 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6709 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6709 SmaI CCC'GGG Cuts at: 1872 3290 4036 1872 Size: 1418 746 4545 Fragments arranged by size: 4545 1418 746 SmlI C'TyrA_G Cuts at: 820 1017 1943 4813 5714 5976 820 Size: 197 926 2870 901 262 1553 Fragments arranged by size: 2870 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4500 493 Size: 4007 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6709 SphI G_CATG'C Cuts at: 1132 2923 4067 1132 Size: 1791 1144 3774 Fragments arranged by size: 3774 1791 1144 SspI AAT'ATT Cuts at: 5 52 2273 4633 5 Size: 47 2221 2360 2081 Fragments arranged by size: 2360 2221 2081 47 StuI AGG'CCT Cuts at: 1517 3261 1517 Size: 1744 4965 StyI C'CwwG_G Cuts at: 513 1065 3150 3311 3336 4148 513 Size: 552 2085 161 25 812 3074 Fragments arranged by size: 3074 2085 812 552 161 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3225 4736 4753 4906 5091 5536 325 Size: 1255 1645 1511 17 153 185 445 1498 Fragments arranged by size: 1645 1511 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3723 4955 6667 Size: 274 80 33 51 1443 1746 1232 1712 Cuts at: 6667 96 Size: 138 Fragments arranged by size: 1746 1712 1443 1232 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 3462 3494 6669 6701 2393 Size: 32 1037 32 3175 32 2401 Fragments arranged by size: 3175 2401 1037 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6709 XbaI T'CTAG_A Cuts at: 4045 4045 Size: 6709 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3955 3955 Size: 6709 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6709 XmaI C'CCGG_G Cuts at: 1870 3288 4034 1870 Size: 1418 746 4545 Fragments arranged by size: 4545 1418 746 XmnI GAAnn'nnTTC Cuts at: 2366 4838 2366 Size: 2472 4237 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4517 276 Size: 53 83 186 936 2983 2468 Fragments arranged by size: 2983 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PspXI RsrII SacII SfiI SfoI SrfI SwaI