(Linear) (Six-base) MAPSORT of: KIEE3695.seq Check: 8965 from: 1 to: 2004 KIEE3695.orf With 182 enzymes: SgfI * March 22, 2012 16:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 130 2004 Size: 130 1874 Acc65I G'GTAC_C Cuts at: 0 1028 2004 Size: 1028 976 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 229 2004 Size: 229 1775 AflIII A'CryG_T Cuts at: 0 288 290 593 2004 Size: 288 2 303 1411 Fragments arranged by size: 1411 303 288 2 AhdI GACnn_n'nnGTC Cuts at: 0 966 2004 Size: 966 1038 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 967 2004 Size: 967 1037 AlwNI CAG_nnn'CTG Cuts at: 0 672 735 963 1899 2004 Size: 672 63 228 936 105 Fragments arranged by size: 936 672 228 105 63 ApaI G_GGCC'C Cuts at: 0 53 2004 Size: 53 1951 ApaLI G'TGCA_C Cuts at: 0 1651 2004 Size: 1651 353 AvaI C'yCGr_G Cuts at: 0 550 1032 1283 2004 Size: 550 482 251 721 Fragments arranged by size: 721 550 482 251 BanI G'GyrC_C Cuts at: 0 1028 1182 1243 1416 2004 Size: 1028 154 61 173 588 Fragments arranged by size: 1028 588 173 154 61 BanII G_rGCy'C Cuts at: 0 53 308 639 830 1366 1465 1633 2004 Size: 53 255 331 191 536 99 168 371 Fragments arranged by size: 536 371 331 255 191 168 99 53 BbsI GAAGACnn'nnnn_ Cuts at: 0 73 2004 Size: 73 1931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1900 1934 2004 Size: 1900 34 70 Fragments arranged by size: 1900 70 34 BglI GCCn_nnn'nGGC Cuts at: 0 57 1191 1370 2004 Size: 57 1134 179 634 Fragments arranged by size: 1134 634 179 57 BglII A'GATC_T Cuts at: 0 1598 2004 Size: 1598 406 BlpI GC'TnA_GC Cuts at: 0 163 1156 1900 2004 Size: 163 993 744 104 Fragments arranged by size: 993 744 163 104 Bme1580I G_kGCm'C Cuts at: 0 53 1421 1655 2004 Size: 53 1368 234 349 Fragments arranged by size: 1368 349 234 53 BmgBI CAC'GTC Cuts at: 0 231 594 903 2004 Size: 231 363 309 1101 Fragments arranged by size: 1101 363 309 231 BmrI ACTGGGnnnn_n' Cuts at: 0 934 1667 2004 Size: 934 733 337 Fragments arranged by size: 934 733 337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 694 904 1443 1603 2004 Size: 82 612 210 539 160 401 Fragments arranged by size: 612 539 401 210 160 82 BsaI GGTCTCn'nnnn_ Cuts at: 0 511 932 1232 1873 2004 Size: 511 421 300 641 131 Fragments arranged by size: 641 511 421 300 131 BsaAI yAC'GTr Cuts at: 0 291 2004 Size: 291 1713 BsaBI GATnn'nnATC Cuts at: 0 202 2004 Size: 202 1802 BsaHI Gr'CG_yC Cuts at: 0 340 487 608 761 1501 2004 Size: 340 147 121 153 740 503 Fragments arranged by size: 740 503 340 153 147 121 BsaWI w'CCGG_w Cuts at: 0 1016 2004 Size: 1016 988 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 388 418 1159 1189 1498 1528 2004 Size: 388 30 741 30 309 30 476 Fragments arranged by size: 741 476 388 309 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 988 991 997 1239 1522 1606 2004 Size: 988 3 6 242 283 84 398 Fragments arranged by size: 988 398 283 242 84 6 3 BseYI C'CCAG_C Cuts at: 0 72 102 877 1211 1402 1852 1892 1925 Size: 72 30 775 334 191 450 40 33 Cuts at: 1925 2004 Size: 79 Fragments arranged by size: 775 450 334 191 79 72 40 33 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 880 2004 Size: 226 654 1124 Fragments arranged by size: 1124 654 226 BsiEI CG_ry'CG Cuts at: 0 582 2004 Size: 582 1422 BsiHKAI G_wGCw'C Cuts at: 0 224 308 788 1157 1633 1655 1906 2004 Size: 224 84 480 369 476 22 251 98 Fragments arranged by size: 480 476 369 251 224 98 84 22 Bsp1286I G_dGCh'C Cuts at: 0 53 224 308 639 788 830 1157 1366 Size: 53 171 84 331 149 42 327 209 Cuts at: 1366 1421 1465 1633 1655 1906 2004 Size: 55 44 168 22 251 98 Fragments arranged by size: 331 327 251 209 171 168 149 98 84 55 53 44 42 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 130 486 971 1061 2004 Size: 130 356 485 90 943 Fragments arranged by size: 943 485 356 130 90 BsrBI CCG'CTC Cuts at: 0 192 869 2004 Size: 192 677 1135 Fragments arranged by size: 1135 677 192 BsrFI r'CCGG_y Cuts at: 0 56 1112 1755 2004 Size: 56 1056 643 249 Fragments arranged by size: 1056 643 249 56 BssHII G'CGCG_C Cuts at: 0 26 2004 Size: 26 1978 BstAPI GCAn_nnn'nTGC Cuts at: 0 963 2004 Size: 963 1041 BstEII G'GTnAC_C Cuts at: 0 1287 2004 Size: 1287 717 BstXI CCAn_nnnn'nTGG Cuts at: 0 1206 2004 Size: 1206 798 BstYI r'GATC_y Cuts at: 0 420 863 1598 2004 Size: 420 443 735 406 Fragments arranged by size: 735 443 420 406 Bsu36I CC'TnA_GG Cuts at: 0 344 1251 2004 Size: 344 907 753 Fragments arranged by size: 907 753 344 BtgI C'CryG_G Cuts at: 0 1145 1727 2004 Size: 1145 582 277 Fragments arranged by size: 1145 582 277 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 453 2004 Size: 453 1551 BtsI GCAGTG_nn' Cuts at: 0 111 2004 Size: 111 1893 DrdI GACnn_nn'nnGTC Cuts at: 0 1530 2004 Size: 1530 474 EaeI y'GGCC_r Cuts at: 0 134 579 1129 1218 2004 Size: 134 445 550 89 786 Fragments arranged by size: 786 550 445 134 89 EagI C'GGCC_G Cuts at: 0 579 2004 Size: 579 1425 EarI CTCTTCn'nnn_ Cuts at: 0 313 2004 Size: 313 1691 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 604 2004 Size: 604 1400 EcoICRI GAG'CTC Cuts at: 0 306 1631 2004 Size: 306 1325 373 Fragments arranged by size: 1325 373 306 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 229 694 904 1443 1603 2004 Size: 82 147 465 210 539 160 401 Fragments arranged by size: 539 465 401 210 160 147 82 EcoNI CCTnn'n_nnAGG Cuts at: 0 1305 1585 2004 Size: 1305 280 419 Fragments arranged by size: 1305 419 280 EcoO109I rG'GnC_Cy Cuts at: 0 50 530 960 1050 1849 2004 Size: 50 480 430 90 799 155 Fragments arranged by size: 799 480 430 155 90 50 EcoRV GAT'ATC Cuts at: 0 1776 2004 Size: 1776 228 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1470 2004 Size: 1470 534 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 952 984 1062 1094 1159 1191 1498 1530 Size: 952 32 78 32 65 32 307 32 Cuts at: 1530 1659 1691 2004 Size: 129 32 313 Fragments arranged by size: 952 313 307 129 78 65 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 150 2004 Size: 150 1854 KpnI G_GTAC'C Cuts at: 0 1032 2004 Size: 1032 972 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 494 1389 1522 1541 2004 Size: 494 895 133 19 463 Fragments arranged by size: 895 494 463 133 19 MscI TGG'CCA Cuts at: 0 136 2004 Size: 136 1868 MslI CAynn'nnrTG Cuts at: 0 1150 1204 2004 Size: 1150 54 800 Fragments arranged by size: 1150 800 54 MspA1I CmG'CkG Cuts at: 0 524 669 1241 1896 2004 Size: 524 145 572 655 108 Fragments arranged by size: 655 572 524 145 108 NcoI C'CATG_G Cuts at: 0 1145 2004 Size: 1145 859 NdeI CA'TA_TG Cuts at: 0 409 802 2004 Size: 409 393 1202 Fragments arranged by size: 1202 409 393 NruI TCG'CGA Cuts at: 0 202 2004 Size: 202 1802 NspI r_CATG'y Cuts at: 0 705 918 2004 Size: 705 213 1086 Fragments arranged by size: 1086 705 213 PflMI CCAn_nnn'nTGG Cuts at: 0 132 2004 Size: 132 1872 PfoI T'CCnGG_A Cuts at: 0 1010 2004 Size: 1010 994 PmlI CAC'GTG Cuts at: 0 291 2004 Size: 291 1713 PpuMI rG'GwC_Cy Cuts at: 0 530 960 1050 2004 Size: 530 430 90 954 Fragments arranged by size: 954 530 430 90 PshAI GACnn'nnGTC Cuts at: 0 1342 2004 Size: 1342 662 PspOMI G'GGCC_C Cuts at: 0 49 2004 Size: 49 1955 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1804 1836 2004 Size: 1804 32 168 Fragments arranged by size: 1804 168 32 PstI C_TGCA'G Cuts at: 0 497 959 2004 Size: 497 462 1045 Fragments arranged by size: 1045 497 462 PvuII CAG'CTG Cuts at: 0 524 669 1896 2004 Size: 524 145 1227 108 Fragments arranged by size: 1227 524 145 108 SacI G_AGCT'C Cuts at: 0 308 1633 2004 Size: 308 1325 371 Fragments arranged by size: 1325 371 308 SanDI GG'GwC_CC Cuts at: 0 530 2004 Size: 530 1474 SapI GCTCTTCn'nnn_ Cuts at: 0 313 2004 Size: 313 1691 SbfI CC_TGCA'GG Cuts at: 0 497 2004 Size: 497 1507 SexAI A'CCwGG_T Cuts at: 0 275 317 2004 Size: 275 42 1687 Fragments arranged by size: 1687 275 42 SfcI C'TryA_G Cuts at: 0 493 955 2004 Size: 493 462 1049 Fragments arranged by size: 1049 493 462 SmaI CCC'GGG Cuts at: 0 1285 2004 Size: 1285 719 SphI G_CATG'C Cuts at: 0 918 2004 Size: 918 1086 SspI AAT'ATT Cuts at: 0 268 2004 Size: 268 1736 StuI AGG'CCT Cuts at: 0 1256 2004 Size: 1256 748 StyI C'CwwG_G Cuts at: 0 1145 1306 1331 2004 Size: 1145 161 25 673 Fragments arranged by size: 1145 673 161 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1220 2004 Size: 1220 784 TatI w'GTAC_w Cuts at: 0 1718 2004 Size: 1718 286 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 388 420 1457 1489 2004 Size: 388 32 1037 32 515 Fragments arranged by size: 1037 515 388 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1950 2004 Size: 1950 54 XmaI C'CCGG_G Cuts at: 0 1283 2004 Size: 1283 721 XmnI GAAnn'nnTTC Cuts at: 0 361 2004 Size: 361 1643 Enzymes that do cut: AarI Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NdeI NruI NspI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SmaI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AleI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BmtI BplI Bpu10I BpuEI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NsiI PacI PasI PciI PmeI PpiI PsiI PspXI PvuI RsrII SacII SalI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI