Restriction map for
pFN21AE1856
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# NO CUT ENZYMES ( 163 enzymes) #
##################################
Acc65I AccI AccII AccIII AciI
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaBI BsaI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 553 GACGTC GACGT^C
AcsI 933 GAATTC [GA]^AATT[CT]
AcyI 553 GACGTC G[GA]^CG[CT]C
AflIII 288 ACACGT A^C[GA][CT]GT
AlwNI 55 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 933 GAATTC [GA]^AATT[CT]
BbrPI 289 CACGTG CAC^GTG
BclI 959 TGATCA T^GATCA
BcnI 129 CCGGG CC^[GC]GG
BfaI 205 CTAG C^TAG
BsaAI 289 CACGTG [CT]AC^GT[GA]
BsaHI 553 GACGTC G[GA]^CG[CT]C
Bsp1407I 147 TGTACA T^GTACA
BspMI 54 GCAGGT ACCTGC(4/8)
BsrFI 111 GCCGGT [GA]^CCGG[CT]
BsrGI 147 TGTACA T^GTACA
BstAPI 245 GCAGAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 267 CCAGCTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 111 GCCGGT [GA]^CCGG[CT]
DraIII 8 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 933 GAATTC G^AATTC
FbaI 959 TGATCA T^GATCA
HgaI 16 GACGC GACGC(5/10)
Hin1I 553 GACGTC G[GA]^CG[CT]C
HindIII 1042 AAGCTT A^AGCTT
MaeI 205 CTAG C^TAG
MfeI 968 CAATTG C^AATTG
MspA1I 268 CAGCTG C[AC]G^C[GT]G
MunI 968 CAATTG C^AATTG
NciI 129 CCGGG CC^[GC]GG
NlaIV 114 GGTCCC GG[ACGT]^[ACGT]CC
PflMI 985 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1054 GAGTC GAGTC(4/5)
PmaCI 289 CACGTG CAC^GTG
PmlI 289 CACGTG CAC^GTG
PvuII 268 CAGCTG CAG^CTG
SmlI 947 CTCAAG C^T[CT][GA]AG
SspBI 147 TGTACA T^GTACA
TfiI 428 GAATC G^A[AT]TC
Van91I 985 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 204 TCTAGA T^CTAGA
XcmI 311 CCAAAGGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 148 GTAC GT^AC
259 GTAC
AvaII 114 GGTCC G^G[AT]CC
796 GGACC
BsgI 5 CTGCAC GTGCAG(16/14)
37 GTGCAG
BsmFI 115 GTCCC GGGAC(10/14)
1081 GTCCC
BstF5I 126 CATCC GGATG(2/0)
906 GGATG
BstYI 225 GGATCT [GA]^GATC[CT]
1032 AGATCC
Cac8I 266 GCCAGC GC[ACGT]^[ACGT]GC
1044 GCTTGC
Cfr13I 114 GGTCC G^G[ACGT]CC
796 GGACC
FokI 126 CATCC GGATG(9/13)
906 GGATG
HaeIII 110 GGCC GG^CC
316 GGCC
HapII 112 CCGG C^CGG
129 CCGG
HinfI 428 GAATC G^A[ACGT]TC
1054 GAGTC
HpaII 112 CCGG C^CGG
129 CCGG
MflI 225 GGATCT [GA]^GATC[CT]
1032 AGATCC
MspI 112 CCGG C^CGG
129 CCGG
RsaI 148 GTAC GT^AC
259 GTAC
Sau96I 114 GGTCC G^G[ACGT]CC
796 GGACC
TspRI 549 CAGTG CA[GC]TG[ACGT][ACGT]^
693 CACTG
XhoII 225 GGATCT [GA]^GATC[CT]
1032 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BbvI BpmI BsiYI
BslI BsrI BssKI BstNI DdeI
DpnII EarI EcoRII Fnu4HI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I