(Circular) (Six-base) MAPSORT of: pFN21AE1856.seq Check: 9117 from: 1 to: 5830 pFN21AE1856 5830 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2050 Size: 5830 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2562 3640 278 Size: 53 83 186 936 1026 1078 2468 Fragments arranged by size: 2468 1078 1026 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2492 2549 3893 5277 5545 1632 Size: 455 405 57 1344 1384 268 1917 Fragments arranged by size: 1917 1384 1344 455 405 268 57 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 2293 4729 5706 1203 Size: 1090 2436 977 1327 Fragments arranged by size: 2436 1327 1090 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5830 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5830 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2640 2672 2640 Size: 32 5798 AlwNI CAG_nnn'CTG Cuts at: 2065 3573 5145 2065 Size: 1508 1572 2750 Fragments arranged by size: 2750 1572 1508 ApaLI G'TGCA_C Cuts at: 3887 5043 3887 Size: 1156 4674 ApoI r'AATT_y Cuts at: 1493 2938 3139 3365 3517 5626 1493 Size: 1445 201 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 201 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 3155 1870 Size: 73 1212 4545 Fragments arranged by size: 4545 1212 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3151 4606 618 Size: 325 217 627 1364 1455 1842 Fragments arranged by size: 1842 1455 1364 627 325 217 BanII G_rGCy'C Cuts at: 1391 3149 1391 Size: 1758 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4859 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3722 4932 3722 Size: 1210 4620 BclI T'GATC_A Cuts at: 1548 2964 1548 Size: 1416 4414 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4441 136 Size: 107 122 71 631 605 2769 1525 Fragments arranged by size: 2769 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 3247 3247 Size: 5830 Bme1580I G_kGCm'C Cuts at: 1790 3891 5047 1790 Size: 2101 1156 2573 Fragments arranged by size: 2573 2101 1156 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 5107 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3561 4519 442 Size: 416 53 83 399 24 2144 958 1753 Fragments arranged by size: 2144 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 3018 3120 4490 1239 Size: 830 949 102 1370 2579 Fragments arranged by size: 2579 1370 949 830 102 Bpu10I CC'TnA_GC Cuts at: 2202 2202 Size: 5830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2937 3955 4820 5118 2937 Size: 1018 865 298 3649 Fragments arranged by size: 3649 1018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4493 882 Size: 617 2994 2219 Fragments arranged by size: 2994 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2296 3621 493 Size: 711 1092 1325 2702 Fragments arranged by size: 2702 1325 1092 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2559 3637 4019 4654 Size: 53 83 186 936 1026 1078 382 635 Cuts at: 4654 275 Size: 1451 Fragments arranged by size: 1451 1078 1026 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5605 Fragments arranged by size: 5605 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5830 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1995 2063 1163 Size: 573 259 68 4930 Fragments arranged by size: 4930 573 259 68 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3280 4041 5069 1540 Size: 275 186 1279 761 1028 2301 Fragments arranged by size: 2301 1279 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3891 3976 5047 3891 Size: 85 1071 4674 Fragments arranged by size: 4674 1071 85 BsmI GAATG_Cn' Cuts at: 3341 3434 3341 Size: 93 5737 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3149 3891 3976 5047 1391 Size: 399 1359 742 85 1071 2174 Fragments arranged by size: 2174 1359 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 3185 844 Size: 1206 1135 3489 Fragments arranged by size: 3489 1206 1135 BsrBI CCG'CTC Cuts at: 1394 1823 3717 1394 Size: 429 1894 3507 Fragments arranged by size: 3507 1894 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2116 4474 4661 1700 Size: 110 306 2358 187 2869 Fragments arranged by size: 2869 2358 306 187 110 BsrGI T'GTAC_A Cuts at: 96 2152 96 Size: 2056 3774 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 3890 4902 3890 Size: 1012 4818 BstAPI GCAn_nnn'nTGC Cuts at: 794 2256 794 Size: 1462 4368 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5830 BstXI CCAn_nnnn'nTGG Cuts at: 2279 2279 Size: 5830 BstYI r'GATC_y Cuts at: 1972 2230 3037 3160 3527 3912 3929 5381 5467 Size: 258 807 123 367 385 17 1452 86 Cuts at: 5467 5479 5825 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 807 385 367 346 258 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2697 3346 4140 4160 2697 Size: 649 794 20 4367 Fragments arranged by size: 4367 794 649 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DraIII CAC_nnn'GTG Cuts at: 2018 2018 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4837 809 Size: 507 3521 1802 Fragments arranged by size: 3521 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3277 4166 5795 Size: 54 1425 50 275 18 1447 889 1629 Cuts at: 5795 8 Size: 43 Fragments arranged by size: 1629 1447 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3277 1537 Size: 275 1465 4090 Fragments arranged by size: 4090 1465 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2063 2786 3051 3760 1353 Size: 710 723 265 709 3423 Fragments arranged by size: 3423 723 710 709 265 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4417 4791 4937 387 Size: 1064 138 2828 374 146 1280 Fragments arranged by size: 2828 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2087 2492 2549 3018 3120 3893 Size: 393 437 18 405 57 469 102 773 Cuts at: 3893 4490 5277 5545 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 773 597 469 437 405 393 268 102 57 18 EcoRI G'AATT_C Cuts at: 2938 3139 3517 2938 Size: 201 378 5251 Fragments arranged by size: 5251 378 201 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 4977 1678 Size: 3299 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2215 2247 4471 4503 4545 Size: 32 135 32 643 32 2224 32 42 Cuts at: 4545 4577 1373 Size: 32 2626 Fragments arranged by size: 2626 2224 643 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 3047 748 Size: 2299 3531 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 2973 3429 2973 Size: 456 5374 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4944 5128 1305 Size: 3639 184 2007 Fragments arranged by size: 3639 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3788 4147 4306 518 Size: 648 2622 359 159 2042 Fragments arranged by size: 2622 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2275 3246 3923 5071 5316 1606 Size: 669 971 677 1148 245 2120 Fragments arranged by size: 2120 1148 971 677 669 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4339 Fragments arranged by size: 4339 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2996 1108 Size: 1888 3942 PfoI T'CCnGG_A Cuts at: 2133 2133 Size: 5830 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PmlI CAC'GTG Cuts at: 2296 2296 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PstI C_TGCA'G Cuts at: 830 3182 830 Size: 2352 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2275 2275 Size: 5830 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 3178 4313 4994 5185 826 Size: 219 2133 1135 681 191 1471 Fragments arranged by size: 2133 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 3157 1872 Size: 1285 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2952 3934 4835 5097 820 Size: 197 926 1009 982 901 262 1553 Fragments arranged by size: 1553 1009 982 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 2004 3269 513 Size: 552 939 1265 3074 Fragments arranged by size: 3074 1265 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 3857 3874 4027 4212 4657 325 Size: 1255 1035 1242 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1242 1035 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2152 2263 4076 Size: 274 80 33 51 1443 175 111 1813 Cuts at: 4076 5788 96 Size: 1712 138 Fragments arranged by size: 1813 1712 1443 274 175 138 111 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5830 XbaI T'CTAG_A Cuts at: 2209 3166 2209 Size: 957 4873 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2323 2323 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 3155 1870 Size: 1285 4545 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2560 3638 276 Size: 53 83 186 936 1026 1078 2468 Fragments arranged by size: 2468 1078 1026 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI