(Circular) (Six-base) MAPSORT of: pFN21AE2383.seq Check: 9396 from: 1 to: 6175 pFN21AE2383 6175 bp With 182 enzymes: SgfI * August 20, 2012 11:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2130 3518 1937 Size: 193 1388 4594 Fragments arranged by size: 4594 1388 193 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3464 4238 5622 5890 1632 Size: 1832 774 1384 268 1917 Fragments arranged by size: 1917 1832 1384 774 268 AfeI AGC'GCT Cuts at: 3184 3184 Size: 6175 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3201 1184 Size: 2017 4158 AlwNI CAG_nnn'CTG Cuts at: 2683 2779 3166 3377 3918 5490 2683 Size: 96 387 211 541 1572 3368 Fragments arranged by size: 3368 1572 541 387 211 96 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 3117 3248 3484 3710 3862 5971 1493 Size: 1624 131 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1624 236 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 2994 4729 160 Size: 872 1962 1735 1606 Fragments arranged by size: 1962 1735 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2945 3500 1870 Size: 73 1002 555 4545 Fragments arranged by size: 4545 1002 555 73 BamHI G'GATC_C Cuts at: 2418 3505 3872 2418 Size: 1087 367 4721 Fragments arranged by size: 4721 1087 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2589 3053 3083 3496 4951 Size: 325 217 627 802 464 30 413 1455 Cuts at: 4951 618 Size: 1842 Fragments arranged by size: 1842 1455 802 627 464 413 325 217 30 BanII G_rGCy'C Cuts at: 1391 2875 3494 1391 Size: 1484 619 4072 Fragments arranged by size: 4072 1484 619 BbeI G_GCGC'C Cuts at: 2593 3057 2593 Size: 464 5711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 3304 928 Size: 971 429 976 3799 Fragments arranged by size: 3799 976 971 429 BbvCI CC'TCA_GC Cuts at: 2818 2818 Size: 6175 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BfrBI ATG'CAT Cuts at: 2269 2509 2269 Size: 240 5935 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 2610 3592 2610 Size: 982 5193 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3906 4864 442 Size: 416 53 83 399 24 2489 958 1753 Fragments arranged by size: 2489 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2695 2988 4835 1239 Size: 1340 116 293 1847 2579 Fragments arranged by size: 2579 1847 1340 293 116 Bpu10I CC'TnA_GC Cuts at: 2818 2818 Size: 6175 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2243 4300 5165 5463 2243 Size: 2057 865 298 2955 Fragments arranged by size: 2955 2057 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3249 4838 882 Size: 617 1750 1589 2219 Fragments arranged by size: 2219 1750 1589 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2590 3054 3093 3982 Size: 53 83 186 936 1057 464 39 889 Cuts at: 3982 4364 4999 275 Size: 382 635 1451 Fragments arranged by size: 1451 1057 936 889 635 464 382 186 83 53 39 BsaWI w'CCGG_w Cuts at: 2154 4608 5006 5280 5427 5838 2154 Size: 2454 398 274 147 411 2491 Fragments arranged by size: 2491 2454 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2961 2991 1178 Size: 30 165 30 1558 30 4362 Fragments arranged by size: 4362 1558 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2698 1186 Size: 1512 4663 BseYI C'CCAG_C Cuts at: 2659 5378 5874 2659 Size: 2719 496 2960 Fragments arranged by size: 2960 2719 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2202 2225 2940 3363 3458 1163 Size: 573 466 23 715 423 95 3880 Fragments arranged by size: 3880 715 573 466 423 95 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4236 4321 5392 4236 Size: 85 1071 5019 Fragments arranged by size: 5019 1071 85 BsmI GAATG_Cn' Cuts at: 2239 2417 2497 2736 2764 3686 3779 2239 Size: 178 80 239 28 922 93 4635 Fragments arranged by size: 4635 922 239 178 93 80 28 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2875 3494 4236 4321 5392 1391 Size: 399 1085 619 742 85 1071 2174 Fragments arranged by size: 2174 1085 1071 742 619 399 85 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 3530 844 Size: 1455 1231 3489 Fragments arranged by size: 3489 1455 1231 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 4670 4844 65 Size: 1183 1239 2183 174 1396 Fragments arranged by size: 2183 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3153 96 Size: 3057 3118 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 2854 2878 4235 5247 2854 Size: 24 1357 1012 3782 Fragments arranged by size: 3782 1357 1012 24 BstAPI GCAn_nnn'nTGC Cuts at: 794 2492 3386 794 Size: 1698 894 3583 Fragments arranged by size: 3583 1698 894 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2719 2317 Size: 402 5773 BstYI r'GATC_y Cuts at: 1972 2070 2418 3505 3872 4257 4274 5726 5812 Size: 98 348 1087 367 385 17 1452 86 Cuts at: 5812 5824 6170 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1087 385 367 348 346 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2523 2886 3087 513 Size: 552 1458 363 201 3601 Fragments arranged by size: 3601 1458 552 363 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2237 2631 2910 3691 4485 4505 2237 Size: 394 279 781 794 20 3907 Fragments arranged by size: 3907 794 781 394 279 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3371 5182 809 Size: 507 2055 1811 1802 Fragments arranged by size: 2055 1811 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2398 3622 4511 Size: 54 1425 50 275 18 568 1224 889 Cuts at: 4511 6140 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1224 889 568 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2542 4105 1353 Size: 1189 1563 3423 Fragments arranged by size: 3423 1563 1189 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2679 4762 5136 5282 387 Size: 1064 138 1090 2083 374 146 1280 Fragments arranged by size: 2083 1280 1090 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 2695 2988 3464 4238 4835 5622 Size: 393 947 116 293 476 774 597 787 Cuts at: 5622 5890 1239 Size: 268 1524 Fragments arranged by size: 1524 947 787 774 597 476 393 293 268 116 EcoNI CCTnn'n_nnAGG Cuts at: 2696 3101 3242 2696 Size: 405 141 5629 Fragments arranged by size: 5629 405 141 EcoO109I rG'GnC_Cy Cuts at: 2429 2429 Size: 6175 EcoRI G'AATT_C Cuts at: 3117 3484 3862 3117 Size: 367 378 5430 Fragments arranged by size: 5430 378 367 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2237 2237 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 2593 2690 2865 3057 3186 5322 1678 Size: 915 97 175 192 129 2136 2531 Fragments arranged by size: 2531 2136 915 192 175 129 97 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2257 2289 2374 2406 4816 Size: 32 135 32 685 32 85 32 2410 Cuts at: 4816 4848 4890 4922 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2410 685 135 85 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KasI G'GCGC_C Cuts at: 2589 3053 2589 Size: 464 5711 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 2067 2334 2959 5289 5473 1305 Size: 739 23 267 625 2330 184 2007 Fragments arranged by size: 2330 2007 739 625 267 184 23 MscI TGG'CCA Cuts at: 10 64 1489 1832 2400 10 Size: 54 1425 343 568 3785 Fragments arranged by size: 3785 1425 568 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 2282 2342 2378 4133 4492 4651 Size: 648 888 228 60 36 1755 359 159 Cuts at: 4651 518 Size: 2042 Fragments arranged by size: 2042 1755 888 648 359 228 159 60 36 MspA1I CmG'CkG Cuts at: 1606 2733 2804 3442 3591 4268 5416 5661 1606 Size: 1127 71 638 149 677 1148 245 2120 Fragments arranged by size: 2120 1148 1127 677 638 245 149 71 NarI GG'CG_CC Cuts at: 2590 3054 2590 Size: 464 5711 NcoI C'CATG_G Cuts at: 513 1065 2523 3087 513 Size: 552 1458 564 3601 Fragments arranged by size: 3601 1458 564 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NsiI A_TGCA'T Cuts at: 2271 2511 2271 Size: 240 5935 NspI r_CATG'y Cuts at: 1132 1229 3533 5078 1132 Size: 97 2304 1545 2229 Fragments arranged by size: 2304 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2866 1348 Size: 1518 4657 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6175 PfoI T'CCnGG_A Cuts at: 2148 2148 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2429 2429 Size: 6175 PshAI GACnn'nnGTC Cuts at: 1613 2157 1613 Size: 544 5631 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 2296 2697 2803 3174 3190 3273 3527 830 Size: 1466 401 106 371 16 83 254 3478 Fragments arranged by size: 3478 1466 401 371 254 106 83 16 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2804 3442 2804 Size: 638 5537 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SapI GCTCTTCn'nnn_ Cuts at: 2542 2542 Size: 6175 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2292 2693 2799 3170 3186 3269 3523 Size: 219 1247 401 106 371 16 83 254 Cuts at: 3523 4658 5339 5530 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1247 1135 681 401 371 254 219 191 106 83 16 SfoI GGC'GCC Cuts at: 2591 3055 2591 Size: 464 5711 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 4279 5180 5442 820 Size: 197 926 279 2057 901 262 1553 Fragments arranged by size: 2057 1553 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 2248 2992 4099 5 Size: 47 2196 744 1107 2081 Fragments arranged by size: 2196 2081 1107 744 47 StuI AGG'CCT Cuts at: 1517 3107 1517 Size: 1590 4585 StyI C'CwwG_G Cuts at: 513 1065 2523 3087 3614 513 Size: 552 1458 564 527 3074 Fragments arranged by size: 3074 1458 564 552 527 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 3116 4202 4219 4372 4557 5002 Size: 1255 1131 405 1086 17 153 185 445 Cuts at: 5002 325 Size: 1498 Fragments arranged by size: 1498 1255 1131 1086 445 405 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3153 4421 6133 Size: 274 80 33 51 1443 1176 1268 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 1712 1443 1268 1176 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6135 6167 6135 Size: 32 6143 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3401 3401 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 4304 4304 Size: 6175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI