Restriction map for
pFN21AE1867
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
Acc65I AccI AccII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsgI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI HinP1I
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AatII 772 GACGTC GACGT^C
AccIII 314 TCCGGA T^CCGGA
AcsI 455 GAATTT [GA]^AATT[CT]
AcyI 772 GACGTC G[GA]^CG[CT]C
ApaI 491 GGGCCC GGGCC^C
ApoI 455 GAATTT [GA]^AATT[CT]
AspHI 511 GTGCTC G[AT]GC[AT]^C
AspI 202 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 239 GGTCC G^G[AT]CC
BamHI 485 GGATCC G^GATCC
BanI 214 GGCACC G^G[CT][GA]CC
BcnI 489 CCGGG CC^[GC]GG
BpmI 473 CTCCAG CTGGAG(16/14)
BsaHI 772 GACGTC G[GA]^CG[CT]C
BsaI 28 GGTCTC GGTCTC(1/5)
BsaWI 314 TCCGGA [AT]^CCGG[AT]
BseAI 314 TCCGGA T^CCGGA
BsiHKAI 511 GTGCTC G[AT]GC[AT]^C
BsmI 393 GAATGC GAATGC(1/-1)
BspEI 314 TCCGGA T^CCGGA
BstEII 178 GGTGACC G^GT[ACGT]ACC
BstPI 178 GGTGACC G^GT[ACGT]ACC
BstXI 631 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 152 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 243 CCATGG C^C[GA][CT]GG
EaeI 669 TGGCCG [CT]^GGCC[GA]
EarI 19 CTCTTC CTCTTC(1/4)
EcoNI 18 CCTCTTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 178 GGTGACC G^GT[ACGT]ACC
Hin1I 772 GACGTC G[GA]^CG[CT]C
HindIII 326 AAGCTT A^AGCTT
Ksp632I 19 CTCTTC CTCTTC(1/4)
MaeII 773 ACGT A^CGT
MroI 314 TCCGGA T^CCGGA
NciI 489 CCGGG CC^[GC]GG
NcoI 243 CCATGG C^CATGG
NspI 113 GCATGC [GA]CATG^[CT]
PflFI 202 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfaNI 732 GATGC GCATC(5/9)
SphI 113 GCATGC GCATG^C
StuI 635 AGGCCT AGG^CCT
TfiI 701 GATTC G^A[AT]TC
Tth111I 202 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 262 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
280 CAGTCACTG
BseRI 16 CTCCTC GAGGAG(10/8)
94 GAGGAG
BspMI 182 ACCTGC ACCTGC(4/8)
565 ACCTGC
BsrI 291 CCAGT ACTGG(1/-1)
656 CCAGT
BstYI 365 AGATCC [GA]^GATC[CT]
485 GGATCC
EcoT14I 243 CCATGG C^C[AT][AT]GG
450 CCAAGG
HapII 315 CCGG C^CGG
489 CCGG
HpaII 315 CCGG C^CGG
489 CCGG
MboII 20 TCTTC GAAGA(8/7)
407 TCTTC
MflI 365 AGATCC [GA]^GATC[CT]
485 GGATCC
MseI 412 TTAA T^TAA
533 TTAA
MspI 315 CCGG C^CGG
489 CCGG
PleI 189 GACTC GAGTC(4/5)
604 GAGTC
PstI 184 CTGCAG CTGCA^G
676 CTGCAG
PvuII 100 CAGCTG CAG^CTG
144 CAGCTG
StyI 243 CCATGG C^C[AT][AT]GG
450 CCAAGG
Tru9I 412 TTAA T^TAA
533 TTAA
Tsp509I 341 AATT ^AATT
456 AATT
XcmI 369 CCAGATATTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
709 CCAAAGCCCGTGTGG
XhoII 365 AGATCC [GA]^GATC[CT]
485 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BanII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfcI TaqI TseI Tsp45I
TspRI TthHB8I