################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BbeI BbrPI BbvCI BciVI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiHKAI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflII 280 CTTAAG C^TTAAG AgeI 182 ACCGGT A^CCGGT AlwNI 656 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 443 GGATCC G^GATCC BanII 673 GGGCTC G[GA]GC[CT]^C BbsI 391 GTCTTC GAAGAC(2/6) BcgI 26 GCACTTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 65 TGATCA T^GATCA BfrI 280 CTTAAG C^TTAAG BmyI 673 GGGCTC G[AGT]GC[ACT]^C BpuAI 391 GTCTTC GAAGAC(2/6) BsaWI 182 ACCGGT [AT]^CCGG[AT] BseRI 549 GAGGAG GAGGAG(10/8) BsgI 24 CTGCAC GTGCAG(16/14) BsiEI 180 CGACCG CG[GA][CT]^CG BsmBI 552 GAGACG CGTCTC(1/5) Bsp1286I 673 GGGCTC G[AGT]GC[ACT]^C BsrFI 182 ACCGGT [GA]^CCGG[CT] BstYI 443 GGATCC [GA]^GATC[CT] Cfr10I 182 ACCGGT [GA]^CCGG[CT] DdeI 310 CTGAG C^T[ACGT]AG DrdI 177 GACCGACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FauI 84 CCCGC CCCGC(4/6) FbaI 65 TGATCA T^GATCA MflI 443 GGATCC [GA]^GATC[CT] MseI 281 TTAA T^TAA MspA1I 692 CAGCTG C[AC]G^C[GT]G PinAI 182 ACCGGT A^CCGGT PvuII 692 CAGCTG CAG^CTG SfcI 244 CTACAG C^T[GA][CT]AG SmlI 280 CTTAAG C^T[CT][GA]AG SspI 406 AATATT AAT^ATT Tru9I 281 TTAA T^TAA XcmI 338 CCATCCGCCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 443 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 204 GTAC GT^AC 558 GTAC AluI 275 AGCT AG^CT 693 AGCT AlwI 444 GATCC GGATC(4/5) 443 GGATC BcnI 45 CCCGG CC^[GC]GG 194 CCCGG BpmI 199 CTCCAG CTGGAG(16/14) 695 CTGGAG BsaI 129 GGTCTC GGTCTC(1/5) 364 GGTCTC BsrI 560 ACTGG ACTGG(1/-1) 201 CCAGT BstF5I 99 CATCC GGATG(2/0) 339 CATCC Cac8I 634 GCCAGC GC[ACGT]^[ACGT]GC 638 GCCTGC CfoI 55 GCGC GCG^C 720 GCGC DraII 80 GGGACCC [GA]G^G[ACGT]CC[CT] 700 GGGTCCC DsaI 447 CCATGG C^C[GA][CT]GG 668 CCATGG EcoNI 240 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 542 CCTGTGAGAGG EcoO109I 80 GGGACCC [GA]G^G[ACGT]CC[CT] 700 GGGTCCC EcoT14I 447 CCATGG C^C[AT][AT]GG 668 CCATGG FokI 99 CATCC GGATG(9/13) 339 CATCC HhaI 55 GCGC GCG^C 720 GCGC HinP1I 55 GCGC G^CGC 720 GCGC HinfI 454 GACTC G^A[ACGT]TC 585 GAGTC MboII 323 GAAGA GAAGA(8/7) 392 TCTTC NciI 45 CCCGG CC^[GC]GG 194 CCCGG NcoI 447 CCATGG C^CATGG 668 CCATGG PleI 454 GACTC GAGTC(4/5) 585 GAGTC PpuMI 80 GGGACCC [GA]G^G[AT]CC[CT] 700 GGGTCCC RsaI 204 GTAC GT^AC 558 GTAC StyI 447 CCATGG C^C[AT][AT]GG 668 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AccII AciI AvaII BbvI BsaJI BsiYI BslI BsmAI BsmFI BssKI BstNI BstUI Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HpaII HphI ItaI MaeIII MboI MnlI MspI MvaI MvnI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI