Restriction map for
pFN21AE2281
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AcsI AcyI AflII
AflIII AocI ApaI ApoI AscI
AseI AsnI AvaI AviII AvrII
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrGI
BssHII BstBI Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eco81I
EcoRI EcoRV FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KspI MamI MfeI MluI MseI
MunI NarI NotI NruI NspV
PacI PaeR7I PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI Tru9I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 820 GGTACC G^GTACC
AccII 68 CGCG CG^CG
AccIII 961 TCCGGA T^CCGGA
AfaI 821 GTAC GT^AC
AgeI 2029 ACCGGT A^CCGGT
AhdI 1402 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2518 GTGCAC G^TGCAC
Aor51HI 1886 AGCGCT AGC^GCT
ApaLI 2518 GTGCAC G^TGCAC
Asp700I 883 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 820 GGTACC G^GTACC
AspEI 1402 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1232 TGGCCA TGG^CCA
BbsI 433 GAAGAC GAAGAC(2/6)
BpuAI 433 GAAGAC GAAGAC(2/6)
BseAI 961 TCCGGA T^CCGGA
BsgI 1079 CTGCAC GTGCAG(16/14)
BspEI 961 TCCGGA T^CCGGA
BspHI 1955 TCATGA T^CATGA
BssSI 1084 CTCGTG CACGAG(-5/-1)
BstEII 1852 GGTCACC G^GT[ACGT]ACC
BstPI 1852 GGTCACC G^GT[ACGT]ACC
BstUI 68 CGCG CG^CG
DsaI 1091 CCATGG C^C[GA][CT]GG
Eam1105I 1402 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1886 AGCGCT AGC^GCT
EcoO65I 1852 GGTCACC G^GT[ACGT]ACC
EcoT22I 811 ATGCAT ATGCA^T
FauI 2493 GCGGG CCCGC(4/6)
KpnI 820 GGTACC GGTAC^C
MluNI 1232 TGGCCA TGG^CCA
MroI 961 TCCGGA T^CCGGA
MscI 1232 TGGCCA TGG^CCA
MvnI 68 CGCG CG^CG
NaeI 1445 GCCGGC GCC^GGC
NcoI 1091 CCATGG C^CATGG
NdeI 2177 CATATG CA^TATG
NgoMIV 1445 GCCGGC G^CCGGC
NheI 63 GCTAGC G^CTAGC
NsiI 811 ATGCAT ATGCA^T
NspI 588 GCATGC [GA]CATG^[CT]
PflFI 1219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2029 ACCGGT A^CCGGT
PstI 2418 CTGCAG CTGCA^G
RcaI 1955 TCATGA T^CATGA
RsaI 821 GTAC GT^AC
SacI 2509 GAGCTC GAGCT^C
SphI 588 GCATGC GCATG^C
Tth111I 1219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 883 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 424 GTATAC GT^[AC][GT]AC
742 GTATAC
BamHI 528 GGATCC G^GATCC
1298 GGATCC
BanI 820 GGTACC G^G[CT][GA]CC
2284 GGTGCC
BclI 782 TGATCA T^GATCA
1062 TGATCA
BglII 72 AGATCT A^GATCT
414 AGATCT
BsaI 458 GAGACC GGTCTC(1/5)
2371 GGTCTC
BsrBI 1038 CCGCTC CCGCTC(-3/-3)
2491 GAGCGG
BsrFI 1445 GCCGGC [GA]^CCGG[CT]
2029 ACCGGT
Bst1107I 424 GTATAC GTA^TAC
742 GTATAC
BstAPI 471 GCAGTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1266 GCATCTGCTGC
BstXI 2048 CCAGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2353 CCAGATGGATGG
BstZ17I 424 GTATAC GTA^TAC
742 GTATAC
Cfr10I 1445 GCCGGC [GA]^CCGG[CT]
2029 ACCGGT
EagI 1443 CGGCCG C^GGCCG
2098 CGGCCG
EclXI 1443 CGGCCG C^GGCCG
2098 CGGCCG
Eco52I 1443 CGGCCG C^GGCCG
2098 CGGCCG
EcoNI 196 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
536 CCTCTCAGAGG
FbaI 782 TGATCA T^GATCA
1062 TGATCA
HaeII 695 GGCGCT [GA]GCGC^[CT]
1886 AGCGCT
HindIII 644 AAGCTT A^AGCTT
787 AAGCTT
MaeII 132 ACGT A^CGT
893 ACGT
MslI 1284 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2278 CACAGGGGTG
PpuMI 190 AGGACCC [GA]G^G[AT]CC[CT]
1016 AGGTCCC
SapI 1668 GAAGAGC GCTCTTC(1/4)
2017 GAAGAGC
SmlI 795 CTCAAG C^T[CT][GA]AG
1549 CTCAAG
XcmI 926 CCATAGTCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2259 CCACTCTAAGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaII BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
TseI Tsp45I Tsp509I TspRI XhoII