(Circular) (Six-base) MAPSORT of: pFN21AE2281.seq Check: 5944 from: 1 to: 7234 pFN21AE2281 7234 bp With 182 enzymes: SgfI * March 28, 2012 12:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5044 278 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 2748 4577 1937 Size: 493 318 1829 4594 Fragments arranged by size: 4594 1829 493 318 Acc65I G'GTAC_C Cuts at: 2825 4555 2825 Size: 1730 5504 AclI AA'CG_TT Cuts at: 1563 5361 5734 1563 Size: 3798 373 3063 Fragments arranged by size: 3798 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3692 3869 5297 6681 6949 1632 Size: 2060 177 1428 1384 268 1917 Fragments arranged by size: 2060 1917 1428 1384 268 177 AfeI AGC'GCT Cuts at: 3893 3893 Size: 7234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7037 AflIII A'CryG_T Cuts at: 1203 6133 7110 1203 Size: 4930 977 1327 Fragments arranged by size: 4930 1327 977 AgeI A'CCGG_T Cuts at: 4034 6065 4034 Size: 2031 5203 AhdI GACnn_n'nnGTC Cuts at: 3412 5963 3412 Size: 2551 4683 AleI CACnn'nnGTG Cuts at: 1166 4287 1166 Size: 3121 4113 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7234 AlwNI CAG_nnn'CTG Cuts at: 2551 3506 3790 4977 6549 2551 Size: 955 284 1187 1572 3236 Fragments arranged by size: 3236 1572 1187 955 284 ApaLI G'TGCA_C Cuts at: 4523 5291 6447 4523 Size: 768 1156 5310 Fragments arranged by size: 5310 1156 768 ApoI r'AATT_y Cuts at: 1493 4543 4769 4921 7030 1493 Size: 3050 226 152 2109 1697 Fragments arranged by size: 3050 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5788 160 Size: 872 4756 1606 Fragments arranged by size: 4756 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 AvaI C'yCGr_G Cuts at: 1870 1943 4559 1870 Size: 73 2616 4545 Fragments arranged by size: 4545 2616 73 BamHI G'GATC_C Cuts at: 2533 3303 4564 4931 2533 Size: 770 1261 367 4836 Fragments arranged by size: 4836 1261 770 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2825 4289 4555 6010 618 Size: 325 217 627 1038 1464 266 1455 1842 Fragments arranged by size: 1842 1464 1455 1038 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 3378 4350 4518 4553 1391 Size: 1987 972 168 35 4072 Fragments arranged by size: 4072 1987 972 168 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2445 928 Size: 971 546 5717 Fragments arranged by size: 5717 971 546 BbvCI CC'TCA_GC Cuts at: 2957 3361 3909 2957 Size: 404 548 6282 Fragments arranged by size: 6282 548 404 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5425 5459 1903 Size: 34 3488 34 3678 Fragments arranged by size: 3678 3488 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5126 6336 5126 Size: 1210 6024 BclI T'GATC_A Cuts at: 1548 2787 3067 1548 Size: 1239 280 5715 Fragments arranged by size: 5715 1239 280 BfrBI ATG'CAT Cuts at: 2818 2818 Size: 7234 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5845 136 Size: 107 122 71 631 605 4173 1525 Fragments arranged by size: 4173 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2077 2419 7229 2077 Size: 342 4810 2082 Fragments arranged by size: 4810 2082 342 BlpI GC'TnA_GC Cuts at: 4651 4651 Size: 7234 Bme1580I G_kGCm'C Cuts at: 1790 4208 4527 5295 6451 1790 Size: 2418 319 768 1156 2573 Fragments arranged by size: 2573 2418 1156 768 319 BmgBI CAC'GTC Cuts at: 1318 2899 1318 Size: 1581 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3530 4965 5923 Size: 416 53 83 399 24 2113 1435 958 Cuts at: 5923 442 Size: 1753 Fragments arranged by size: 2113 1753 1435 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2072 1056 Size: 1016 6218 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2237 2299 2592 2725 3387 3417 3621 Size: 937 61 62 293 133 662 30 204 Cuts at: 3621 3668 3975 4271 4392 5894 1239 Size: 47 307 296 121 1502 2579 Fragments arranged by size: 2579 1502 937 662 307 296 293 204 133 121 62 61 47 30 Bpu10I CC'TnA_GC Cuts at: 2921 2957 3361 3791 3909 4353 2921 Size: 36 404 430 118 444 5802 Fragments arranged by size: 5802 444 430 404 118 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2785 3539 5359 6224 6522 2785 Size: 754 1820 865 298 3497 Fragments arranged by size: 3497 1820 865 754 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2457 4382 5897 882 Size: 617 958 1925 1515 2219 Fragments arranged by size: 2219 1925 1515 958 617 BsaAI yAC'GTr Cuts at: 493 1204 5025 493 Size: 711 3821 2702 Fragments arranged by size: 3821 2702 711 BsaBI GATnn'nnATC Cuts at: 7228 7228 Size: 7234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5041 5423 6058 275 Size: 53 83 186 936 3508 382 635 1451 Fragments arranged by size: 3508 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2966 4034 4504 5667 6065 6339 6486 6897 2966 Size: 1068 470 1163 398 274 147 411 3303 Fragments arranged by size: 3303 1163 1068 470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2221 2235 2251 2265 2297 Size: 30 165 30 818 14 16 14 32 Cuts at: 2297 2327 3812 3842 1178 Size: 30 1485 30 4570 Fragments arranged by size: 4570 1485 818 165 32 30 30 30 30 16 14 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2338 2879 3321 3710 3926 3978 4501 4504 Size: 1152 541 442 389 216 52 523 3 Cuts at: 4504 1186 Size: 3916 Fragments arranged by size: 3916 1152 541 523 442 389 216 52 3 BseYI C'CCAG_C Cuts at: 3025 3198 3339 3704 3733 4042 6437 6933 3025 Size: 173 141 365 29 309 2395 496 3326 Fragments arranged by size: 3326 2395 496 365 309 173 141 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3069 1163 Size: 573 1333 5328 Fragments arranged by size: 5328 1333 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4035 4106 4684 5445 6473 Size: 275 186 1450 584 71 578 761 1028 Cuts at: 6473 1540 Size: 2301 Fragments arranged by size: 2301 1450 1028 761 584 578 275 186 71 BsiHKAI G_wGCw'C Cuts at: 2382 3060 4518 4527 5295 5380 6451 2382 Size: 678 1458 9 768 85 1071 3165 Fragments arranged by size: 3165 1458 1071 768 678 85 9 BsmI GAATG_Cn' Cuts at: 2113 2820 3795 4745 4838 2113 Size: 707 975 950 93 4509 Fragments arranged by size: 4509 975 950 707 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 3060 3378 4208 4350 4518 4527 Size: 399 592 678 318 830 142 168 9 Cuts at: 4527 4553 5295 5380 6451 1391 Size: 26 742 85 1071 2174 Fragments arranged by size: 2174 1071 830 742 678 592 399 318 168 142 85 26 9 BspEI T'CCGG_A Cuts at: 2966 6897 2966 Size: 3931 3303 BspHI T'CATG_A Cuts at: 3960 5123 6853 3960 Size: 1163 1730 4341 Fragments arranged by size: 4341 1730 1163 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4589 844 Size: 3745 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3045 4498 5121 1394 Size: 429 1222 1453 623 3507 Fragments arranged by size: 3507 1453 1222 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2380 4065 4339 5729 5903 65 Size: 1183 1132 1685 274 1390 174 1396 Fragments arranged by size: 1685 1396 1390 1183 1132 274 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3450 4034 5878 6065 1700 Size: 110 1640 584 1844 187 2869 Fragments arranged by size: 2869 1844 1640 584 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7234 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7234 BssSI C'ACGA_G Cuts at: 3089 5294 6306 3089 Size: 2205 1012 4017 Fragments arranged by size: 4017 2205 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2482 3277 794 Size: 1688 795 4751 Fragments arranged by size: 4751 1688 795 BstBI TT'CG_AA Cuts at: 6038 6038 Size: 7234 BstEII G'GTnAC_C Cuts at: 1184 3857 1184 Size: 2673 4561 BstXI CCAn_nnnn'nTGG Cuts at: 2011 4060 4365 2011 Size: 2049 305 4880 Fragments arranged by size: 4880 2049 305 BstYI r'GATC_y Cuts at: 1972 2077 2419 2533 3303 4564 4931 5316 5333 Size: 105 342 114 770 1261 367 385 17 Cuts at: 5333 6785 6871 6883 7229 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1261 770 385 367 346 342 114 105 86 17 12 BstZ17I GTA'TAC Cuts at: 2431 2749 2431 Size: 318 6916 BtgI C'CryG_G Cuts at: 513 1065 3096 513 Size: 552 2031 4651 Fragments arranged by size: 4651 2031 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6495 Fragments arranged by size: 6495 683 56 BtsI GCAGTG_nn' Cuts at: 4123 4750 5544 5564 4123 Size: 627 794 20 5793 Fragments arranged by size: 5793 794 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7234 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2827 2862 529 Size: 35 2263 35 4901 Fragments arranged by size: 4901 2263 35 35 DraI TTT'AAA Cuts at: 4538 4538 Size: 7234 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6241 809 Size: 507 4925 1802 Fragments arranged by size: 4925 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3237 3448 4103 Size: 54 1425 50 275 18 1407 211 655 Cuts at: 4103 4681 5570 7199 8 Size: 578 889 1629 43 Fragments arranged by size: 1629 1425 1407 889 655 578 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 4103 4681 1537 Size: 275 1636 655 578 4090 Fragments arranged by size: 4090 1636 655 578 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2883 3488 3668 4017 5164 1353 Size: 1530 605 180 349 1147 3423 Fragments arranged by size: 3423 1530 1147 605 349 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3469 4131 5821 6195 6341 387 Size: 1064 138 1880 662 1690 374 146 1280 Fragments arranged by size: 1880 1690 1280 1064 662 374 146 138 EcoICRI GAG'CTC Cuts at: 4516 4516 Size: 7234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2237 2299 2592 2725 3387 3417 Size: 393 544 61 62 293 133 662 30 Cuts at: 3417 3621 3668 3692 3869 3975 4271 4392 5297 Size: 204 47 24 177 106 296 121 905 Cuts at: 5297 5894 6681 6949 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 905 787 662 597 544 393 296 293 268 204 177 133 121 106 62 61 47 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 2205 2545 2205 Size: 340 6894 EcoO109I rG'GnC_Cy Cuts at: 2196 3022 3571 4171 4384 2196 Size: 826 549 600 213 5046 Fragments arranged by size: 5046 826 600 549 213 EcoRI G'AATT_C Cuts at: 4543 4921 4543 Size: 378 6856 FspI TGC'GCA Cuts at: 5740 5740 Size: 7234 HaeII r_GCGC'y Cuts at: 1678 2704 3895 6381 1678 Size: 1026 1191 2486 2531 Fragments arranged by size: 2531 2486 1191 1026 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 2518 2525 2550 Size: 32 135 32 663 32 251 7 25 Cuts at: 2550 2557 3365 3397 3398 3430 5875 5907 5949 Size: 7 808 32 1 32 2445 32 42 Cuts at: 5949 5981 1373 Size: 32 2626 Fragments arranged by size: 2626 2445 808 663 251 135 42 32 32 32 32 32 32 32 25 7 7 1 HincII GTy'rAC Cuts at: 669 1657 1938 4578 4824 669 Size: 988 281 2640 246 3079 Fragments arranged by size: 3079 2640 988 281 246 HindIII A'AGCT_T Cuts at: 748 2649 2792 748 Size: 1901 143 5190 Fragments arranged by size: 5190 1901 143 HpaI GTT'AAC Cuts at: 4824 4824 Size: 7234 KpnI G_GTAC'C Cuts at: 2829 4559 2829 Size: 1730 5504 MfeI C'AATT_G Cuts at: 4833 4833 Size: 7234 MluI A'CGCG_T Cuts at: 7110 7110 Size: 7234 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3820 6348 6532 1305 Size: 2515 2528 184 2007 Fragments arranged by size: 2528 2515 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3239 10 Size: 54 1425 343 1407 4005 Fragments arranged by size: 4005 1425 1407 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3293 4287 5192 5551 5710 518 Size: 648 2127 994 905 359 159 2042 Fragments arranged by size: 2127 2042 994 905 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 3365 3633 3704 3737 4650 5327 6475 Size: 651 1108 268 71 33 913 677 1148 Cuts at: 6475 6720 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1108 913 677 651 268 245 71 33 NaeI GCC'GGC Cuts at: 3452 3452 Size: 7234 NcoI C'CATG_G Cuts at: 513 1065 3096 513 Size: 552 2031 4651 Fragments arranged by size: 4651 2031 552 NdeI CA'TA_TG Cuts at: 387 4183 387 Size: 3796 3438 NgoMIV G'CCGG_C Cuts at: 3450 3450 Size: 7234 NheI G'CTAG_C Cuts at: 1052 2068 1052 Size: 1016 6218 NotI GC'GGCC_GC Cuts at: 4681 4681 Size: 7234 NsiI A_TGCA'T Cuts at: 2820 2820 Size: 7234 NspI r_CATG'y Cuts at: 1132 1229 2597 4592 6137 1132 Size: 97 1368 1995 1545 2229 Fragments arranged by size: 2229 1995 1545 1368 97 PasI CC'CwG_GG Cuts at: 1348 4082 1348 Size: 2734 4500 PciI A'CATG_T Cuts at: 6133 6133 Size: 7234 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7234 PfoI T'CCnGG_A Cuts at: 2192 2192 Size: 7234 PmeI GTTT'AAAC Cuts at: 4538 4538 Size: 7234 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5303 5335 6816 6848 5303 Size: 32 1481 32 5689 Fragments arranged by size: 5689 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2196 3022 2196 Size: 826 6408 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7234 PsiI TTA'TAA Cuts at: 4804 4804 Size: 7234 PstI C_TGCA'G Cuts at: 830 4427 4586 830 Size: 3597 159 3478 Fragments arranged by size: 3597 3478 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7234 PvuII CAG'CTG Cuts at: 3365 3704 3737 3365 Size: 339 33 6862 Fragments arranged by size: 6862 339 33 SacI G_AGCT'C Cuts at: 4518 4518 Size: 7234 SalI G'TCGA_C Cuts at: 1936 4576 1936 Size: 2640 4594 SapI GCTCTTCn'nnn_ Cuts at: 3668 4017 3668 Size: 349 6885 SbfI CC_TGCA'GG Cuts at: 4586 4586 Size: 7234 ScaI AGT'ACT Cuts at: 5482 7194 5482 Size: 1712 5522 SfcI C'TryA_G Cuts at: 826 1045 4327 4339 4423 4582 5717 6398 6589 Size: 219 3282 12 84 159 1135 681 191 Cuts at: 6589 826 Size: 1471 Fragments arranged by size: 3282 1471 1135 681 219 191 159 84 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7234 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7234 SmaI CCC'GGG Cuts at: 1872 4561 1872 Size: 2689 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2800 3554 5338 6239 6501 820 Size: 197 926 857 754 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 857 754 262 197 SnaBI TAC'GTA Cuts at: 493 5025 493 Size: 4532 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7234 SphI G_CATG'C Cuts at: 1132 2597 4592 1132 Size: 1465 1995 3774 Fragments arranged by size: 3774 1995 1465 SspI AAT'ATT Cuts at: 5 52 5158 5 Size: 47 5106 2081 Fragments arranged by size: 5106 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7234 StyI C'CwwG_G Cuts at: 513 1065 2493 2605 3096 3117 3540 4673 513 Size: 552 1428 112 491 21 423 1133 3074 Fragments arranged by size: 3074 1428 1133 552 491 423 112 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2512 5261 5278 5431 5616 6061 325 Size: 1255 932 2749 17 153 185 445 1498 Fragments arranged by size: 2749 1498 1255 932 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5480 7192 96 Size: 274 80 33 51 1443 3503 1712 138 Fragments arranged by size: 3503 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2297 2329 7194 7226 2297 Size: 32 4865 32 2305 Fragments arranged by size: 4865 2305 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3227 1316 Size: 1911 5323 XbaI T'CTAG_A Cuts at: 4570 4570 Size: 7234 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2938 4271 2938 Size: 1333 5901 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7234 XmaI C'CCGG_G Cuts at: 1870 4559 1870 Size: 2689 4545 XmnI GAAnn'nnTTC Cuts at: 2892 5363 2892 Size: 2471 4763 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5042 276 Size: 53 83 186 936 3508 2468 Fragments arranged by size: 3508 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI