(Linear) (Six-base) MAPSORT of: KIEE2281.seq Check: 9084 from: 1 to: 2529 KIEE2281.orf With 182 enzymes: SgfI * March 22, 2012 16:01 .. AccI GT'mk_AC Cuts at: 0 425 743 2529 Size: 425 318 1786 Fragments arranged by size: 1786 425 318 Acc65I G'GTAC_C Cuts at: 0 820 2529 Size: 820 1709 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1687 1864 2529 Size: 1687 177 665 Fragments arranged by size: 1687 665 177 AfeI AGC'GCT Cuts at: 0 1888 2529 Size: 1888 641 AgeI A'CCGG_T Cuts at: 0 2029 2529 Size: 2029 500 AhdI GACnn_n'nnGTC Cuts at: 0 1407 2529 Size: 1407 1122 AleI CACnn'nnGTG Cuts at: 0 2282 2529 Size: 2282 247 AlwNI CAG_nnn'CTG Cuts at: 0 546 1501 1785 2529 Size: 546 955 284 744 Fragments arranged by size: 955 744 546 284 ApaLI G'TGCA_C Cuts at: 0 2518 2529 Size: 2518 11 BamHI G'GATC_C Cuts at: 0 528 1298 2529 Size: 528 770 1231 Fragments arranged by size: 1231 770 528 BanI G'GyrC_C Cuts at: 0 820 2284 2529 Size: 820 1464 245 Fragments arranged by size: 1464 820 245 BanII G_rGCy'C Cuts at: 0 1373 2345 2513 2529 Size: 1373 972 168 16 Fragments arranged by size: 1373 972 168 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 440 2529 Size: 440 2089 BbvCI CC'TCA_GC Cuts at: 0 952 1356 1904 2529 Size: 952 404 548 625 Fragments arranged by size: 952 625 548 404 BclI T'GATC_A Cuts at: 0 782 1062 2529 Size: 782 280 1467 Fragments arranged by size: 1467 782 280 BfrBI ATG'CAT Cuts at: 0 813 2529 Size: 813 1716 BglII A'GATC_T Cuts at: 0 72 414 2529 Size: 72 342 2115 Fragments arranged by size: 2115 342 72 Bme1580I G_kGCm'C Cuts at: 0 2203 2522 2529 Size: 2203 319 7 Fragments arranged by size: 2203 319 7 BmgBI CAC'GTC Cuts at: 0 894 2529 Size: 894 1635 BmrI ACTGGGnnnn_n' Cuts at: 0 1525 2529 Size: 1525 1004 BmtI G_CTAG'C Cuts at: 0 67 2529 Size: 67 2462 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 232 294 587 720 1382 1412 1616 Size: 171 61 62 293 133 662 30 204 Cuts at: 1616 1663 1970 2266 2387 2529 Size: 47 307 296 121 142 Fragments arranged by size: 662 307 296 293 204 171 142 133 121 62 61 47 30 Bpu10I CC'TnA_GC Cuts at: 0 916 952 1356 1786 1904 2348 2529 Size: 916 36 404 430 118 444 181 Fragments arranged by size: 916 444 430 404 181 118 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 780 1534 2529 Size: 780 754 995 Fragments arranged by size: 995 780 754 BsaI GGTCTCn'nnnn_ Cuts at: 0 452 2377 2529 Size: 452 1925 152 Fragments arranged by size: 1925 452 152 BsaWI w'CCGG_w Cuts at: 0 961 2029 2499 2529 Size: 961 1068 470 30 Fragments arranged by size: 1068 961 470 30 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 216 230 246 260 292 322 1807 1837 Size: 216 14 16 14 32 30 1485 30 Cuts at: 1837 2529 Size: 692 Fragments arranged by size: 1485 692 216 32 30 30 16 14 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 333 874 1316 1705 1921 1973 2496 2499 Size: 333 541 442 389 216 52 523 3 Cuts at: 2499 2529 Size: 30 Fragments arranged by size: 541 523 442 389 333 216 52 30 3 BseYI C'CCAG_C Cuts at: 0 1020 1193 1334 1699 1728 2037 2529 Size: 1020 173 141 365 29 309 492 Fragments arranged by size: 1020 492 365 309 173 141 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1064 2529 Size: 1064 1465 BsiEI CG_ry'CG Cuts at: 0 1446 2030 2101 2529 Size: 1446 584 71 428 Fragments arranged by size: 1446 584 428 71 BsiHKAI G_wGCw'C Cuts at: 0 377 1055 2513 2522 2529 Size: 377 678 1458 9 7 Fragments arranged by size: 1458 678 377 9 7 BsmI GAATG_Cn' Cuts at: 0 108 815 1790 2529 Size: 108 707 975 739 Fragments arranged by size: 975 739 707 108 Bsp1286I G_dGCh'C Cuts at: 0 377 1055 1373 2203 2345 2513 2522 2529 Size: 377 678 318 830 142 168 9 7 Fragments arranged by size: 830 678 377 318 168 142 9 7 BspEI T'CCGG_A Cuts at: 0 961 2529 Size: 961 1568 BspHI T'CATG_A Cuts at: 0 1955 2529 Size: 1955 574 BsrBI CCG'CTC Cuts at: 0 1040 2493 2529 Size: 1040 1453 36 Fragments arranged by size: 1453 1040 36 BsrDI GCAATG_nn' Cuts at: 0 375 2060 2334 2529 Size: 375 1685 274 195 Fragments arranged by size: 1685 375 274 195 BsrFI r'CCGG_y Cuts at: 0 1445 2029 2529 Size: 1445 584 500 Fragments arranged by size: 1445 584 500 BssSI C'ACGA_G Cuts at: 0 1084 2529 Size: 1084 1445 BstAPI GCAn_nnn'nTGC Cuts at: 0 477 1272 2529 Size: 477 795 1257 Fragments arranged by size: 1257 795 477 BstEII G'GTnAC_C Cuts at: 0 1852 2529 Size: 1852 677 BstXI CCAn_nnnn'nTGG Cuts at: 0 2055 2360 2529 Size: 2055 305 169 Fragments arranged by size: 2055 305 169 BstYI r'GATC_y Cuts at: 0 72 414 528 1298 2529 Size: 72 342 114 770 1231 Fragments arranged by size: 1231 770 342 114 72 BstZ17I GTA'TAC Cuts at: 0 426 744 2529 Size: 426 318 1785 Fragments arranged by size: 1785 426 318 BtgI C'CryG_G Cuts at: 0 1091 2529 Size: 1091 1438 BtsI GCAGTG_nn' Cuts at: 0 2118 2529 Size: 2118 411 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 822 857 2529 Size: 822 35 1672 Fragments arranged by size: 1672 822 35 EaeI y'GGCC_r Cuts at: 0 1232 1443 2098 2529 Size: 1232 211 655 431 Fragments arranged by size: 1232 655 431 211 EagI C'GGCC_G Cuts at: 0 1443 2098 2529 Size: 1443 655 431 Fragments arranged by size: 1443 655 431 EarI CTCTTCn'nnn_ Cuts at: 0 878 1483 1663 2012 2529 Size: 878 605 180 349 517 Fragments arranged by size: 878 605 517 349 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1464 2126 2529 Size: 1464 662 403 Fragments arranged by size: 1464 662 403 EcoICRI GAG'CTC Cuts at: 0 2511 2529 Size: 2511 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 171 232 294 587 720 1382 1412 1616 Size: 171 61 62 293 133 662 30 204 Cuts at: 1616 1663 1687 1864 1970 2266 2387 2529 Size: 47 24 177 106 296 121 142 Fragments arranged by size: 662 296 293 204 177 171 142 133 121 106 62 61 47 30 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 200 540 2529 Size: 200 340 1989 Fragments arranged by size: 1989 340 200 EcoO109I rG'GnC_Cy Cuts at: 0 191 1017 1566 2166 2379 2529 Size: 191 826 549 600 213 150 Fragments arranged by size: 826 600 549 213 191 150 HaeII r_GCGC'y Cuts at: 0 699 1890 2529 Size: 699 1191 639 Fragments arranged by size: 1191 699 639 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 230 262 513 520 545 552 1360 1392 Size: 230 32 251 7 25 7 808 32 Cuts at: 1392 1393 1425 2529 Size: 1 32 1104 Fragments arranged by size: 1104 808 251 230 32 32 32 25 7 7 1 HindIII A'AGCT_T Cuts at: 0 644 787 2529 Size: 644 143 1742 Fragments arranged by size: 1742 644 143 KpnI G_GTAC'C Cuts at: 0 824 2529 Size: 824 1705 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1815 2529 Size: 1815 714 MscI TGG'CCA Cuts at: 0 1234 2529 Size: 1234 1295 MslI CAynn'nnrTG Cuts at: 0 1288 2282 2529 Size: 1288 994 247 Fragments arranged by size: 1288 994 247 MspA1I CmG'CkG Cuts at: 0 252 1360 1628 1699 1732 2529 Size: 252 1108 268 71 33 797 Fragments arranged by size: 1108 797 268 252 71 33 NaeI GCC'GGC Cuts at: 0 1447 2529 Size: 1447 1082 NcoI C'CATG_G Cuts at: 0 1091 2529 Size: 1091 1438 NdeI CA'TA_TG Cuts at: 0 2178 2529 Size: 2178 351 NgoMIV G'CCGG_C Cuts at: 0 1445 2529 Size: 1445 1084 NheI G'CTAG_C Cuts at: 0 63 2529 Size: 63 2466 NsiI A_TGCA'T Cuts at: 0 815 2529 Size: 815 1714 NspI r_CATG'y Cuts at: 0 592 2529 Size: 592 1937 PasI CC'CwG_GG Cuts at: 0 2077 2529 Size: 2077 452 PfoI T'CCnGG_A Cuts at: 0 187 2529 Size: 187 2342 PpuMI rG'GwC_Cy Cuts at: 0 191 1017 2529 Size: 191 826 1512 Fragments arranged by size: 1512 826 191 PstI C_TGCA'G Cuts at: 0 2422 2529 Size: 2422 107 PvuII CAG'CTG Cuts at: 0 1360 1699 1732 2529 Size: 1360 339 33 797 Fragments arranged by size: 1360 797 339 33 SacI G_AGCT'C Cuts at: 0 2513 2529 Size: 2513 16 SapI GCTCTTCn'nnn_ Cuts at: 0 1663 2012 2529 Size: 1663 349 517 Fragments arranged by size: 1663 517 349 SfcI C'TryA_G Cuts at: 0 2322 2334 2418 2529 Size: 2322 12 84 111 Fragments arranged by size: 2322 111 84 12 SmlI C'TyrA_G Cuts at: 0 795 1549 2529 Size: 795 754 980 Fragments arranged by size: 980 795 754 SphI G_CATG'C Cuts at: 0 592 2529 Size: 592 1937 StyI C'CwwG_G Cuts at: 0 488 600 1091 1112 1535 2529 Size: 488 112 491 21 423 994 Fragments arranged by size: 994 491 488 423 112 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 507 2529 Size: 507 2022 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 292 324 2529 Size: 292 32 2205 Fragments arranged by size: 2205 292 32 Tth111I GACn'n_nGTC Cuts at: 0 1222 2529 Size: 1222 1307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 933 2266 2529 Size: 933 1333 263 Fragments arranged by size: 1333 933 263 XmnI GAAnn'nnTTC Cuts at: 0 887 2529 Size: 887 1642 Enzymes that do cut: AccI Acc65I AcuI AfeI AgeI AhdI AleI AlwNI ApaLI BamHI BanI BanII BbsI BbvCI BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NsiI NspI PasI PfoI PpuMI PstI PvuII SacI SapI SfcI SmlI SphI StyI TaqII TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AclI AflII AflIII AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BglI BlpI BplI BsaAI BsaBI BsaHI BsiWI BsmBI BspMI BsrGI BssHII BstBI Bsu36I BtgZI ClaI DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI MfeI MluI NarI NotI NruI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XhoI XmaI ZraI