################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbrPI BbvCI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I ClaI DraI DraIII Eam1105I Eco81I EcoNI EcoRI EcoRV FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MunI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I TthHB8I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Alw44I 397 GTGCAC G^TGCAC Aor51HI 752 AGCGCT AGC^GCT ApaI 311 GGGCCC GGGCC^C ApaLI 397 GTGCAC G^TGCAC AspI 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BciVI 218 GTATCC GTATCC(6/5) BfaI 766 CTAG C^TAG BglI 662 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 1085 GAGACC GGTCTC(1/5) BsiEI 745 CGGCCG CG[GA][CT]^CG BsmI 281 GCATTC GAATGC(1/-1) BsrBI 82 GAGCGG CCGCTC(-3/-3) BstAPI 399 GCACGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 879 GGTGACC G^GT[ACGT]ACC BstPI 879 GGTGACC G^GT[ACGT]ACC CpoI 185 CGGACCG CG^G[AT]CCG DraII 310 GGGGCCC [GA]G^G[ACGT]CC[CT] DrdI 157 GACGTTTCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1115 CCATGG C^C[GA][CT]GG EagI 745 CGGCCG C^GGCCG EclXI 745 CGGCCG C^GGCCG Eco47III 752 AGCGCT AGC^GCT Eco52I 745 CGGCCG C^GGCCG EcoO109I 310 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 879 GGTGACC G^GT[ACGT]ACC EcoT14I 1115 CCATGG C^C[AT][AT]GG EcoT22I 853 ATGCAT ATGCA^T HgaI 191 GCGTC GACGC(5/10) HindIII 22 AAGCTT A^AGCTT MaeI 766 CTAG C^TAG MaeIII 880 GTGAC ^GT[ACGT]AC MslI 640 CACTATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 1115 CCATGG C^CATGG NsiI 853 ATGCAT ATGCA^T PflFI 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 828 CTGCAG CTGCA^G RsrII 185 CGGACCG CG^G[AT]CCG SapI 570 GCTCTTC GCTCTTC(1/4) SmlI 25 CTTGAG C^T[CT][GA]AG StyI 1115 CCATGG C^C[AT][AT]GG Tsp45I 880 GTGAC ^GT[GC]AC Tth111I 187 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 608 GGCGCC G[GA]^CG[CT]C 846 GGCGCC AlwNI 595 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG 793 CAGGAGCTG AvaI 359 CCCGGG C^[CT]CG[GA]G 1037 CCCGGG BbeI 608 GGCGCC GGCGC^C 846 GGCGCC BbsI 1098 GAAGAC GAAGAC(2/6) 545 GTCTTC BlpI 498 GCTGAGC GC^T[ACGT]AGC 760 GCTCAGC Bpu1102I 498 GCTGAGC GC^T[ACGT]AGC 760 GCTCAGC BpuAI 1098 GAAGAC GAAGAC(2/6) 545 GTCTTC BsaHI 608 GGCGCC G[GA]^CG[CT]C 846 GGCGCC BsgI 484 CTGCAC GTGCAG(16/14) 712 GTGCAG BsoBI 359 CCCGGG C^[CT]CG[GA]G 1037 CCCGGG BssSI 169 CACGAG CACGAG(-5/-1) 487 CACGAG BstF5I 447 GGATG GGATG(2/0) 467 GGATG CelII 498 GCTGAGC GC^T[ACGT]AGC 760 GCTCAGC EaeI 745 CGGCCG [CT]^GGCC[GA] 941 CGGCCA EarI 571 CTCTTC CTCTTC(1/4) 64 GAAGAG FokI 447 GGATG GGATG(9/13) 467 GGATG Hin1I 608 GGCGCC G[GA]^CG[CT]C 846 GGCGCC KasI 608 GGCGCC G^GCGCC 846 GGCGCC Ksp632I 571 CTCTTC CTCTTC(1/4) 64 GAAGAG NaeI 555 GCCGGC GCC^GGC 781 GCCGGC NarI 608 GGCGCC GG^CGCC 846 GGCGCC NgoMIV 555 GCCGGC G^CCGGC 781 GCCGGC PflMI 594 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 944 CCACTCACTGG PvuII 133 CAGCTG CAG^CTG 831 CAGCTG SfcI 425 CTGTAG C^T[GA][CT]AG 828 CTGCAG SfoI 608 GGCGCC GGC^GCC 846 GGCGCC SmaI 359 CCCGGG CCC^GGG 1037 CCCGGG Van91I 594 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 944 CCACTCACTGG XmaCI 359 CCCGGG C^CCGGG 1037 CCCGGG XmaI 359 CCCGGG C^CCGGG 1037 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 65 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI TseI TspRI