(Circular) (Six-base) MAPSORT of: pFN21AE3157.seq Check: 6610 from: 1 to: 5830 pFN21AE3157 5830 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3640 278 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3173 1937 Size: 1236 4594 Acc65I G'GTAC_C Cuts at: 3151 3151 Size: 5830 AclI AA'CG_TT Cuts at: 1563 3957 4330 1563 Size: 2394 373 3063 Fragments arranged by size: 3063 2394 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2558 3893 5277 5545 1632 Size: 456 470 1335 1384 268 1917 Fragments arranged by size: 1917 1384 1335 470 456 268 AfeI AGC'GCT Cuts at: 2759 2759 Size: 5830 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5633 AflIII A'CryG_T Cuts at: 1203 4729 5706 1203 Size: 3526 977 1327 Fragments arranged by size: 3526 1327 977 AgeI A'CCGG_T Cuts at: 4661 4661 Size: 5830 AhdI GACnn_n'nnGTC Cuts at: 4559 4559 Size: 5830 AleI CACnn'nnGTG Cuts at: 1166 2649 1166 Size: 1483 4347 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2778 2883 1184 Size: 1594 105 4131 Fragments arranged by size: 4131 1594 105 AlwNI CAG_nnn'CTG Cuts at: 2605 2803 3573 5145 2605 Size: 198 770 1572 3290 Fragments arranged by size: 3290 1572 770 198 ApaI G_GGCC'C Cuts at: 2320 2320 Size: 5830 ApaLI G'TGCA_C Cuts at: 2402 3887 5043 2402 Size: 1485 1156 3189 Fragments arranged by size: 3189 1485 1156 ApoI r'AATT_y Cuts at: 1493 3139 3365 3517 5626 1493 Size: 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 226 152 AseI AT'TA_AT Cuts at: 160 1032 4384 160 Size: 872 3352 1606 Fragments arranged by size: 3352 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 AvaI C'yCGr_G Cuts at: 1870 1943 2364 3042 3155 1870 Size: 73 421 678 113 4545 Fragments arranged by size: 4545 678 421 113 73 BamHI G'GATC_C Cuts at: 3160 3527 3160 Size: 367 5463 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2279 2613 2851 3151 4606 Size: 325 217 627 492 334 238 300 1455 Cuts at: 4606 618 Size: 1842 Fragments arranged by size: 1842 1455 627 492 334 325 300 238 217 BanII G_rGCy'C Cuts at: 1391 2320 2510 2577 2778 3149 1391 Size: 929 190 67 201 371 4072 Fragments arranged by size: 4072 929 371 201 190 67 BbeI G_GCGC'C Cuts at: 2617 2855 2617 Size: 238 5592 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2543 3110 928 Size: 971 644 567 3648 Fragments arranged by size: 3648 971 644 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4021 4055 1903 Size: 34 2084 34 3678 Fragments arranged by size: 3678 2084 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2234 3722 4932 2234 Size: 1488 1210 3132 Fragments arranged by size: 3132 1488 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5830 BfrBI ATG'CAT Cuts at: 2860 2860 Size: 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2673 4441 136 Size: 107 122 71 631 605 1001 1768 1525 Fragments arranged by size: 1768 1525 1001 631 605 122 107 71 BglII A'GATC_T Cuts at: 5825 5825 Size: 5830 BlpI GC'TnA_GC Cuts at: 2504 2766 3247 2504 Size: 262 481 5087 Fragments arranged by size: 5087 481 262 Bme1580I G_kGCm'C Cuts at: 1790 2282 2308 2320 2406 3891 5047 1790 Size: 492 26 12 86 1485 1156 2573 Fragments arranged by size: 2573 1485 1156 492 86 26 12 BmgBI CAC'GTC Cuts at: 1318 2269 2407 2740 1318 Size: 951 138 333 4408 Fragments arranged by size: 4408 951 333 138 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2245 3561 4519 Size: 416 53 83 399 24 828 1316 958 Cuts at: 4519 442 Size: 1753 Fragments arranged by size: 1753 1316 958 828 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2173 2173 Size: 5830 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2411 2647 2690 2825 2916 4490 1239 Size: 884 288 236 43 135 91 1574 2579 Fragments arranged by size: 2579 1574 884 288 236 135 91 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2051 3955 4820 5118 2051 Size: 1904 865 298 2763 Fragments arranged by size: 2763 1904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3084 4493 882 Size: 617 1585 1409 2219 Fragments arranged by size: 2219 1585 1409 617 BsaAI yAC'GTr Cuts at: 493 1204 3621 493 Size: 711 2417 2702 Fragments arranged by size: 2702 2417 711 BsaBI GATnn'nnATC Cuts at: 5824 5824 Size: 5830 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2614 2852 3637 4019 Size: 53 83 186 936 1081 238 785 382 Cuts at: 4019 4654 275 Size: 635 1451 Fragments arranged by size: 1451 1081 936 785 635 382 238 186 83 53 BsaWI w'CCGG_w Cuts at: 4263 4661 4935 5082 5493 4263 Size: 398 274 147 411 4600 Fragments arranged by size: 4600 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2952 2982 1178 Size: 30 165 30 1549 30 4026 Fragments arranged by size: 4026 1549 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2192 2960 3113 1186 Size: 1006 768 153 3903 Fragments arranged by size: 3903 1006 768 153 BseYI C'CCAG_C Cuts at: 5033 5529 5033 Size: 496 5334 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2474 2738 1163 Size: 573 738 264 4255 Fragments arranged by size: 4255 738 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2753 3280 4041 5069 1540 Size: 275 186 752 527 761 1028 2301 Fragments arranged by size: 2301 1028 761 752 527 275 186 BsiHKAI G_wGCw'C Cuts at: 2406 2510 2577 2778 3891 3976 5047 2406 Size: 104 67 201 1113 85 1071 3189 Fragments arranged by size: 3189 1113 1071 201 104 85 67 BsmI GAATG_Cn' Cuts at: 2286 3341 3434 2286 Size: 1055 93 4682 Fragments arranged by size: 4682 1055 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2282 2308 2320 2406 2510 2577 2778 Size: 399 492 26 12 86 104 67 201 Cuts at: 2778 3149 3891 3976 5047 1391 Size: 371 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 492 399 371 201 104 86 85 67 26 12 BspEI T'CCGG_A Cuts at: 5493 5493 Size: 5830 BspHI T'CATG_A Cuts at: 3719 5449 3719 Size: 1730 4100 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3185 844 Size: 2341 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2089 3717 1394 Size: 429 266 1628 3507 Fragments arranged by size: 3507 1628 429 266 BsrDI GCAATG_nn' Cuts at: 65 1248 4325 4499 65 Size: 1183 3077 174 1396 Fragments arranged by size: 3077 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2282 2560 2786 2986 4474 4661 1700 Size: 110 472 278 226 200 1488 187 2869 Fragments arranged by size: 2869 1488 472 278 226 200 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5830 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5830 BssSI C'ACGA_G Cuts at: 2174 2492 3890 4902 2174 Size: 318 1398 1012 3102 Fragments arranged by size: 3102 1398 1012 318 BstAPI GCAn_nnn'nTGC Cuts at: 794 2410 794 Size: 1616 4214 BstBI TT'CG_AA Cuts at: 4634 4634 Size: 5830 BstEII G'GTnAC_C Cuts at: 1184 2884 1184 Size: 1700 4130 BstYI r'GATC_y Cuts at: 1972 3160 3527 3912 3929 5381 5467 5479 5825 Size: 1188 367 385 17 1452 86 12 346 Cuts at: 5825 1972 Size: 1977 Fragments arranged by size: 1977 1452 1188 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3120 513 Size: 552 2055 3223 Fragments arranged by size: 3223 2055 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5091 Fragments arranged by size: 5091 683 56 BtsI GCAGTG_nn' Cuts at: 2243 3346 4140 4160 2243 Size: 1103 794 20 3913 Fragments arranged by size: 3913 1103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5830 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5795 DraI TTT'AAA Cuts at: 3134 3134 Size: 5830 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2168 4837 809 Size: 507 852 2669 1802 Fragments arranged by size: 2669 1802 852 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2750 2946 3277 Size: 54 1425 50 275 18 920 196 331 Cuts at: 3277 4166 5795 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 920 889 331 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2750 3277 1537 Size: 275 938 527 4090 Fragments arranged by size: 4090 938 527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2582 3760 1353 Size: 711 518 1178 3423 Fragments arranged by size: 3423 1178 711 518 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2557 4417 4791 4937 387 Size: 1064 138 968 1860 374 146 1280 Fragments arranged by size: 1860 1280 1064 968 374 146 138 EcoICRI GAG'CTC Cuts at: 2508 2575 2776 2508 Size: 67 201 5562 Fragments arranged by size: 5562 201 67 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2123 2411 2558 2647 2690 2825 Size: 393 456 35 288 147 89 43 135 Cuts at: 2825 2916 3893 4490 5277 5545 1239 Size: 91 977 597 787 268 1524 Fragments arranged by size: 1524 977 787 597 456 393 288 268 147 135 91 89 43 35 EcoO109I rG'GnC_Cy Cuts at: 2316 2316 Size: 5830 EcoRI G'AATT_C Cuts at: 3139 3517 3139 Size: 378 5452 FspI TGC'GCA Cuts at: 4336 4336 Size: 5830 HaeII r_GCGC'y Cuts at: 1678 2349 2617 2761 2827 2855 2969 4977 1678 Size: 671 268 144 66 28 114 2008 2531 Fragments arranged by size: 2531 2008 671 268 144 114 66 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4471 4503 4545 4577 1373 Size: 32 135 32 2899 32 42 32 2626 Fragments arranged by size: 2899 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3174 3420 669 Size: 988 281 1236 246 3079 Fragments arranged by size: 3079 1236 988 281 246 HindIII A'AGCT_T Cuts at: 748 2027 748 Size: 1279 4551 HpaI GTT'AAC Cuts at: 3420 3420 Size: 5830 KasI G'GCGC_C Cuts at: 2613 2851 2613 Size: 238 5592 KpnI G_GTAC'C Cuts at: 3155 3155 Size: 5830 MfeI C'AATT_G Cuts at: 3429 3429 Size: 5830 MluI A'CGCG_T Cuts at: 5706 5706 Size: 5830 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2886 4944 5128 1305 Size: 1581 2058 184 2007 Fragments arranged by size: 2058 2007 1581 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4008 Fragments arranged by size: 4008 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2649 3788 4147 4306 518 Size: 648 1483 1139 359 159 2042 Fragments arranged by size: 2042 1483 1139 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2140 2832 2838 3246 3923 5071 5316 1606 Size: 534 692 6 408 677 1148 245 2120 Fragments arranged by size: 2120 1148 692 677 534 408 245 6 NaeI GCC'GGC Cuts at: 2562 2788 2562 Size: 226 5604 NarI GG'CG_CC Cuts at: 2614 2852 2614 Size: 238 5592 NcoI C'CATG_G Cuts at: 513 1065 3120 513 Size: 552 2055 3223 Fragments arranged by size: 3223 2055 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5830 NgoMIV G'CCGG_C Cuts at: 2560 2786 2560 Size: 226 5604 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5830 NotI GC'GGCC_GC Cuts at: 3277 3277 Size: 5830 NsiI A_TGCA'T Cuts at: 2862 2862 Size: 5830 NspI r_CATG'y Cuts at: 1132 1229 3188 4733 1132 Size: 97 1959 1545 2229 Fragments arranged by size: 2229 1959 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5830 PciI A'CATG_T Cuts at: 4729 4729 Size: 5830 PflMI CCAn_nnn'nTGG Cuts at: 1108 2605 2955 1108 Size: 1497 350 3983 Fragments arranged by size: 3983 1497 350 PmeI GTTT'AAAC Cuts at: 3134 3134 Size: 5830 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3899 3931 5412 5444 3899 Size: 32 1481 32 4285 Fragments arranged by size: 4285 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5830 PsiI TTA'TAA Cuts at: 3400 3400 Size: 5830 PspOMI G'GGCC_C Cuts at: 2316 2316 Size: 5830 PstI C_TGCA'G Cuts at: 830 2837 3182 830 Size: 2007 345 3478 Fragments arranged by size: 3478 2007 345 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5830 PvuII CAG'CTG Cuts at: 2140 2838 2140 Size: 698 5132 RsrII CG'GwC_CG Cuts at: 2191 2191 Size: 5830 SacI G_AGCT'C Cuts at: 2510 2577 2778 2510 Size: 67 201 5562 Fragments arranged by size: 5562 201 67 SalI G'TCGA_C Cuts at: 1936 3172 1936 Size: 1236 4594 SapI GCTCTTCn'nnn_ Cuts at: 2582 2582 Size: 5830 SbfI CC_TGCA'GG Cuts at: 3182 3182 Size: 5830 ScaI AGT'ACT Cuts at: 4078 5790 4078 Size: 1712 4118 SfcI C'TryA_G Cuts at: 826 1045 2430 2833 3178 4313 4994 5185 826 Size: 219 1385 403 345 1135 681 191 1471 Fragments arranged by size: 1471 1385 1135 681 403 345 219 191 SfoI GGC'GCC Cuts at: 2615 2853 2615 Size: 238 5592 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5830 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5830 SmaI CCC'GGG Cuts at: 1872 2366 3044 3157 1872 Size: 494 678 113 4545 Fragments arranged by size: 4545 678 494 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 2030 3934 4835 5097 820 Size: 197 926 87 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 87 SnaBI TAC'GTA Cuts at: 493 3621 493 Size: 3128 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5830 SphI G_CATG'C Cuts at: 1132 3188 1132 Size: 2056 3774 SspI AAT'ATT Cuts at: 5 52 3754 5 Size: 47 3702 2081 Fragments arranged by size: 3702 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5830 StyI C'CwwG_G Cuts at: 513 1065 3120 3269 513 Size: 552 2055 149 3074 Fragments arranged by size: 3074 2055 552 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2158 3857 3874 4027 4212 4657 325 Size: 1255 578 1699 17 153 185 445 1498 Fragments arranged by size: 1699 1498 1255 578 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2142 4076 5788 Size: 274 80 33 51 1443 165 1934 1712 Cuts at: 5788 96 Size: 138 Fragments arranged by size: 1934 1712 1443 274 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5790 5822 5790 Size: 32 5798 Tth111I GACn'n_nGTC Cuts at: 1316 2195 1316 Size: 879 4951 XbaI T'CTAG_A Cuts at: 3166 3166 Size: 5830 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5830 XmaI C'CCGG_G Cuts at: 1870 2364 3042 3155 1870 Size: 494 678 113 4545 Fragments arranged by size: 4545 678 494 113 XmnI GAAnn'nnTTC Cuts at: 3959 3959 Size: 5830 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3638 276 Size: 53 83 186 936 2104 2468 Fragments arranged by size: 2468 2104 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PpuMI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI XcmI