Restriction map for
pFN21AE1778
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 305 GAATTC [GA]^AATT[CT]
Alw44I 720 GTGCAC G^TGCAC
AlwI 250 GATCC GGATC(4/5)
AlwNI 586 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 720 GTGCAC G^TGCAC
ApoI 305 GAATTC [GA]^AATT[CT]
AspHI 720 GTGCAC G[AT]GC[AT]^C
BclI 755 TGATCA T^GATCA
BfaI 487 CTAG C^TAG
BglII 539 AGATCT A^GATCT
BlpI 662 GCTTAGC GC^T[ACGT]AGC
BmyI 720 GTGCAC G[AGT]GC[ACT]^C
BpmI 592 CTGGAG CTGGAG(16/14)
Bpu1102I 662 GCTTAGC GC^T[ACGT]AGC
BsaI 420 GAGACC GGTCTC(1/5)
BsiHKAI 720 GTGCAC G[AT]GC[AT]^C
Bsp1286I 720 GTGCAC G[AGT]GC[ACT]^C
BsrDI 743 CATTGC GCAATG(2/0)
BstYI 539 AGATCT [GA]^GATC[CT]
CelII 662 GCTTAGC GC^T[ACGT]AGC
DraI 204 TTTAAA TTT^AAA
DraII 53 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 553 CCACGG C^C[GA][CT]GG
EarI 136 GAAGAG CTCTTC(1/4)
EcoO109I 53 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 305 GAATTC G^AATTC
EcoRV 217 GATATC GAT^ATC
FbaI 755 TGATCA T^GATCA
HgaI 71 GACGC GACGC(5/10)
HindIII 429 AAGCTT A^AGCTT
HinfI 832 GAATC G^A[ACGT]TC
HphI 247 GGTGA GGTGA(8/7)
Ksp632I 136 GAAGAG CTCTTC(1/4)
MaeI 487 CTAG C^TAG
MaeIII 637 GTTAC ^GT[ACGT]AC
MflI 539 AGATCT [GA]^GATC[CT]
NlaIII 468 CATG CATG^
NspI 467 GCATGC [GA]CATG^[CT]
PstI 75 CTGCAG CTGCA^G
SphI 467 GCATGC GCATG^C
SspI 199 AATATT AAT^ATT
TaqI 81 TCGA T^CGA
TfiI 832 GAATC G^A[AT]TC
TthHB8I 81 TCGA T^CGA
XhoII 539 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BseRI 620 GAGGAG GAGGAG(10/8)
643 GAGGAG
BstF5I 778 CATCC GGATG(2/0)
210 GGATG
Cac8I 467 GCATGC GC[ACGT]^[ACGT]GC
700 GCAGGC
DraIII 269 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
650 CACTTTGTG
DrdI 40 GACATCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
71 GACGCTGCAGTC
EcoT14I 35 CCAAGG C^C[AT][AT]GG
131 CCTTGG
FokI 778 CATCC GGATG(9/13)
210 GGATG
MseI 205 TTAA T^TAA
709 TTAA
NlaIV 259 GGGGCC GG[ACGT]^[ACGT]CC
676 GGAGCC
StyI 35 CCAAGG C^C[AT][AT]GG
131 CCTTGG
Tru9I 205 TTAA T^TAA
709 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BbvI BsaJI BsmAI BsmI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI NdeII Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp509I TspRI