(Linear) (Six-base) MAPSORT of: KIEE1778.seq  Check: 8671  from: 1  to: 855



KIEE1778.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:47  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     591     855 
   Size:        591     264
 
ApaLI G'TGCA_C

Cuts at:      0     720     855 
   Size:        720     135
 
ApoI r'AATT_y

Cuts at:      0     305     855 
   Size:        305     550
 
BclI T'GATC_A

Cuts at:      0     755     855 
   Size:        755     100
 
BglII A'GATC_T

Cuts at:      0     539     855 
   Size:        539     316
 
BlpI GC'TnA_GC

Cuts at:      0     663     855 
   Size:        663     192
 
Bme1580I G_kGCm'C

Cuts at:      0     724     855 
   Size:        724     131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613     855 
   Size:        613     242
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     414     855 
   Size:        414     441
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     316     346     374     404     855 
   Size:        316      30      28      30     451

  Fragments arranged by size: 

                 451     316      30      30      28
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     635     658     855 
   Size:        635      23     197

  Fragments arranged by size: 

                 635     197      23
 
BseYI C'CCAG_C

Cuts at:      0     300     581     855 
   Size:        300     281     274

  Fragments arranged by size: 

                 300     281     274
 
BsiHKAI G_wGCw'C

Cuts at:      0     724     855 
   Size:        724     131
 
BsmI GAATG_Cn'

Cuts at:      0     232     348     747     855 
   Size:        232     116     399     108

  Fragments arranged by size: 

                 399     232     116     108
 
Bsp1286I G_dGCh'C

Cuts at:      0     724     855 
   Size:        724     131
 
BsrDI GCAATG_nn'

Cuts at:      0     742     855 
   Size:        742     113
 
BstYI r'GATC_y

Cuts at:      0     539     855 
   Size:        539     316
 
BtgI C'CryG_G

Cuts at:      0     553     855 
   Size:        553     302
 
DraI TTT'AAA

Cuts at:      0     206     855 
   Size:        206     649
 
DraIII CAC_nnn'GTG

Cuts at:      0     274     655     855 
   Size:        274     381     200

  Fragments arranged by size: 

                 381     274     200
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      46      77     855 
   Size:         46      31     778

  Fragments arranged by size: 

                 778      46      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     855 
   Size:        131     724
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     613     855 
   Size:        613     242
 
EcoO109I rG'GnC_Cy

Cuts at:      0      54     855 
   Size:         54     801
 
EcoRI G'AATT_C

Cuts at:      0     305     855 
   Size:        305     550
 
EcoRV GAT'ATC

Cuts at:      0     219     855 
   Size:        219     636
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     147     855 
   Size:        147     708
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     786     818     855 
   Size:        786      32      37

  Fragments arranged by size: 

                 786      37      32
 
HindIII A'AGCT_T

Cuts at:      0     429     855 
   Size:        429     426
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     654     855 
   Size:        654     201
 
NspI r_CATG'y

Cuts at:      0     471     855 
   Size:        471     384
 
PstI C_TGCA'G

Cuts at:      0      79     855 
   Size:         79     776
 
SfcI C'TryA_G

Cuts at:      0      75     116     347     855 
   Size:         75      41     231     508

  Fragments arranged by size: 

                 508     231      75      41
 
SphI G_CATG'C

Cuts at:      0     471     855 
   Size:        471     384
 
SspI AAT'ATT

Cuts at:      0     201     855 
   Size:        201     654
 
StyI C'CwwG_G

Cuts at:      0      35     131     855 
   Size:         35      96     724

  Fragments arranged by size: 

                 724      96      35
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     316     348     855 
   Size:        316      32     507

  Fragments arranged by size: 

                 507     316      32
 

 Enzymes that do cut:

    AlwNI    ApaLI     ApoI     BclI    BglII     BlpI Bme1580I     BpmI
     BsaI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI
    BstYI     BtgI     DraI   DraIII     DrdI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I  HindIII     MmeI     NspI     PstI
     SfcI     SphI     SspI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     EaeI
     EagI     EciI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI